Dentin matrix metalloproteinases (MMPs) are a family of host-derived proteolytic enzymes trapped within mineralized dentin matrix, which have the ability to hydrolyze the organic matrix of demineralized dentin. After bonding with resins to dentin there are usually some exposed collagen fibrils at the bottom of the hybrid layer owing to imperfect resin impregnation of the demineralized dentin matrix. Exposed collagen fibrils might be affected by MMPs inducing hydrolytic degradation, which might result in reduced bond strength. Most MMPs are synthesized and released from odontoblasts in the form of proenzymes, requiring activation to degrade extracellular matrix components. Unfortunately, they can be activated by modem self-etch and etch-and-rinse adhesives. The aim of this review is to summarize the current knowledge of the role of dentinal host-derived MMPs in dentin matrix degradation. We also discuss various available MMP inhibitors, especially chlorhexidine, and suggest that they could provide a potential pathway for inhibiting collagen degradation in bonding interfaces thereby increasing dentin bonding durability.
Chlorhexidine ; pharmacology ; Collagen ; metabolism ; ultrastructure ; Dental Bonding ; Dentin ; enzymology ; ultrastructure ; Dentin-Bonding Agents ; chemistry ; Enzyme Inhibitors ; pharmacology ; Humans ; Hydrolysis ; Matrix Metalloproteinase Inhibitors ; Matrix Metalloproteinases ; metabolism ; Resin Cements ; chemistry

PURPOSE: This in-vitro study was designed to evaluate retention forces, microleakage and plastic deformation of a prefabricated 2-implant bar attachment system (SFI-Bar, Cendres+Metaux, Switzerland). MATERIALS AND METHODS: Two SFI implant-adapters were torqued with 35 Ncm into two implant analogues. Before the tube bars were finally sealed, the inner cavity of the tube bar was filled with liquid red dye to evaluate microleakage. As tube bar sealing agents three different materials were used (AGC Cem (AGC, resin based), Cervitec Plus (CP; varnish) and Gapseal (GS; silicone based). Four groups with eight specimens each were tested (GS, GS+AGC, AGC, CP). For cyclic loading, the attachment system was assembled parallel to the female counterparts in a chewing simulator. The mean retention forces of the initial and final ten cycles were statistically evaluated (ANOVA, alpha< or =.05). RESULTS: All groups showed a significant loss of retention forces. Their means differed between 30-39 N initially and 22-28 N after 50,000 loading cycles. No significant statistical differences could be found between the groups at the beginning (P=.224), at the end (P=.257) or between the loss of retention forces (P=.288). Microleakage occurred initially only in some groups but after 10,000 loading cycles all groups exhibited microleakage. CONCLUSION: Long-term retention forces of the SFI-Bar remained above 20 N which can be considered clinically sufficient. The sealing agents in this study are not suitable to prevent microleakage.
Female ; Humans ; Mastication ; Plastics* ; Silicones

STATEMENT OF THE PROBLEM: All-ceramic post-and-core restorations offer a number of advantages compared with systems that use metal build-ups. In certain clinical cases, however, fractures at the joint between the post and core build-up have been reported. PURPOSE: The objective, therefore, is to improve the joint between the post and the core build-up. MATERIAL AND METHODS: Three different methods were used to prepare all-ceramic post-andcore restorations; pressing IPS Empress core build-ups to CosmoPost zirconia posts, cementing IPS Empress core build-ups to CosmoPost zirconia posts and Celay-milling of zirconia blanks. A series of ten restorations was prepared for each of the three methods. The post-and-core complexes were tested to failure with the load applied perpendicular to the post axis. The load and deflection at fracture were recorded. RESULTS: The highest breaking load and highest deflection were recorded for the cementing technique with values of 25.3 N and 394 micrometer, respectively. The corresponding values for the pressed core build-ups and the milled zirconia core build-ups were 22 N and 301 micrometer, and 13 N and 160 micrometer, respectively. All the differences are statistically significant (p=0.05). Regarding the load-dependence of the deflection, the cemented core build-ups again demonstrated the highest value with 15.5 micrometer/N. The difference in the values of 13.6 micrometer/N and 13 micrometer/N recorded for the pressed-on and milled core build-ups, respectively, were statistically insignificant. CONCLUSION: In regard to the high fracture resistance of zirconia post, adhesive cementing the core build-up to the post offers a viable alternative to the conventional pressing technique. The elastic bond between the rigid high-strength zirconia post and the core build-up presents an additional advantage.
Adhesives ; Axis, Cervical Vertebra ; Joints

OBJECTIVE: The aim of the current study was to evaluate the effect of ultraviolet (UV) photofunctionalization of dental titanium implants with exposure to the oral cavity on osseointegration in an animal model. METHODS: Forty-eight titanium implants (Camlog Conelog 4.3 mmx9.0 mm) were placed epicrestally into the edentulous jaws of three minipigs and implant stability was assessed by measuring the implant stability quotient (ISQ). Prior to implantation half of the implants were photofunctionalized with intense UV-light. After three months, the implants were exposed and ISQ was measured again. After six months of implant exposure, the minipigs were sacrificed and the harvested specimens were analyzed using histomorphometric, light, and fluorescence microscopy. MAIN RESULTS: Forty-two of 48 implants osseointegrated. The overall mean bone-implant contact area (BIC) was (64±22)%. No significant differences were found in BIC or ISQ value (multivariate analysis of variance (MANOVA), P>0.05) between implants with and without exposure to UV photofunctionalization. CONCLUSIONS No significant effects were observed on osseointegration of dental titanium implants nine months after exposure of UV photofunctionalization.
Animals ; Dental Implantation, Endosseous ; methods ; Dental Implants ; Equipment Failure Analysis ; Female ; Hydrophobic and Hydrophilic Interactions ; Male ; Models, Animal ; Osseointegration ; Surface Properties ; Swine ; Swine, Miniature ; Titanium ; Ultraviolet Rays