1.Detection of MMP-11 from menstrual blood using immunohistochemistry.
Ya-Nan YAO ; Hui-Ling LU ; Sen CHEN ; Jing ZHENG ; Ya-Qing ZHANG
Journal of Forensic Medicine 2008;24(1):32-33
OBJECTIVE:
To prove the feasibility of detecting menstrual blood as well as its cellular localization with rabbit-anti-human matrix metalloproteinase-11 (MMP-11) polyclonal antibody.
METHODS:
MMP-11 in menstrual blood, peripheral blood, vaginal liquid, aged menstrual bloodstain, and endometrium sections were assayed with SAP immunohistochemistry.
RESULTS:
MMP-11 was found only in menstrual samples within stroma and epithelium cells.
CONCLUSION
MMP-11 polyclonal antibody may be applied in the distinction between menstrual blood and venous blood.
Adult
;
Endometrium/pathology*
;
Female
;
Forensic Medicine/methods*
;
Humans
;
Immunohistochemistry
;
Matrix Metalloproteinase 11/analysis*
;
Menstrual Cycle/blood*