1.The cytotoxicity of microglass fibers on alveolar macrophages of fischer 344 rats evaluated by cell magnetometry, cytochemisry and morphology.
Hisako SHINJI ; Mitsuyasu WATANABE ; Yuichiro KUDO ; Masato NIITSUYA ; Masashi TSUNODA ; Toshihiko SATOH ; Yasuhiro SAKAI ; Makoto KOTANI ; Yoshiharu AIZAWA
Environmental Health and Preventive Medicine 2005;10(2):111-119
OBJECTIVESThe toxicity of microglass fibers (MG), one of the man-made mineral fibers, has not been sufficiently evaluated. The aim of the current study was to evaluate the cytotoxicity of MGin vitro.
METHODSAlveolar macrophages were obtained from the bronchoalveolar lavage of male F344/N rats. The macrophages were exposed to MG at concentrations of 0, 40, 80, 160 and 320 μg/ml. The effects of MG on the macrophages were examined by cell magnetometry, LDH assay and morphological observation.
RESULTSIn the cell magnetometry experiment, a significant delay of relaxation (the reduction of remanent magnetic field strength) was observed in the cells treated with 160 and 320 μg/ml of MG in a dose-dependent manner. A significant increase in LDH release was also observed in the cells with 160 and 320 μg/ml in a dose-dependent manner. Changes in the cytoskeleton were observed after exposure to MG by immunofluorescent microscopy using an α-tubulin antibody.
CONCLUSIONSThe cytotoxicity of MG on alveolar macrophages was demonstrated with cell magnetometry. The mechanism of the toxic effects of MG was related to cytoskeleton damage.
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