1.Food and Drug Interactions: Effect of Acanthopanax senticosus Harms on CYP3A4 and CYP2C9 Activities (Part 3)
Tsunehisa TAKAHASHI ; Masaki IGARASHI ; Takashi SATOH ; Kazuhiro WATANABE
Japanese Journal of Complementary and Alternative Medicine 2014;11(1):17-24
Objective: By using human liver microsomes (HLM), we analyzed the effects of 14 known components of A.senticosus Harms on the activities of CYP2C9 and CYP3A4.
Methods and Results: Sesamin and quercetin inhibited both enzyme activities, whereas quercitrin strongly inhibited CYP3A4 activity. The 50% inhibitory concentrations (IC50s) of sesamin and quercetin on CYP2C9 activity were approximately 124- and 59-fold higher and the IC50s of sesamin, quercetin, and quercitrin on CYP3A4 activity were approximately 427-, 135-, and 22-fold higher than that of A. senticosus Harms extract (ASE), respectively. All these components inhibited both CYP3A4 and CYP2C9 in a non-competitive manner. However, these components are present in small amounts in ASE.
Conclusion: Therefore, the food-drug interactions caused by A. senticosus Harms are presumed to be due to the additive or synergistic interaction of these components or the other existing components, including their metabolites.
2.Black Tea Inhibits Small Intestinal α-Glucosidase Activity in db/db Mouse
Masaki IGARASHI ; Takashi SATOH ; Hiroshi YAMASHITA ; Kazuhiro WATANABE
Japanese Journal of Complementary and Alternative Medicine 2014;11(1):25-33
The inhibitory effects of the freeze-dried powder of the aqueous extract of black tea leaf (JAT) on α-glucosidase activity were investigated. We initially examined the effects of JAT addition on yeast α-glucosidase activity. JAT significantly and dose-dependently inhibited α-glucosidase activity and more strongly inhibited the activity than acarbose, the positive control. Then, we examined the effects of oral administration of JAT on sucrose tolerance in type 2 diabetes mellitus model db/db mice. Both JAT and acarbose administered groups showed a dose-dependent decrease in plasma glucose levels after the sucrose loading compared with the control group. Notable was that the plasma glucose levels of the 500 mg/kg JAT administered group exhibited a significant decrease 30 min or longer after the sucrose loading. On the other hand, no significant difference in plasma insulin levels was seen between the JAT administered group and the control group. We also measured small intestinal sucrase activity in db/db mouse at 30 min after JAT oral administration. Compared to control mice, small intestinal sucrase activity was significantly decreased in the 500 mg/kg JAT administered mice. These findings indicate that JAT may be a useful natural material for the prevention and therapy of type 2 diabetes mellitus.
3.Inhibitory Effect of Keishi-bukuryo-gan on CGRP-induced Elevation of Skin Temperature in GnRH Analogue-treated Male Rats.
Mitsutoshi YUZURIHARA ; Masamichi NOGUCHI ; Yasushi IGARASHI ; Yoshio KASE ; Shuich TAKEDA ; Masaki ABURADA
Kampo Medicine 2003;54(4):791-795
The gonadotropin-releasing hormone (GnRH) analogue (Leuplin/®1.0mg/kg, s. c.) induced not only a decrease in the serum concentration of testosterone but also potentiation of calcitonin gene-related peptide (CGRP: 10μg/kg, i. v.)-induced elevation of skin temperature in male rats. Keishi-bukuryo-gan (1, 000mg/kg, p. o.) and 17β-estradiol (0.010mg/kg, s. c.) significantly inhibited the elevation of skin temperature as well as teststerone (1.0mg/kg, s. c.) replacement. However, Keishi-bukuryo-gan and 17β-estradiol did not affect the low concentration of serum testosterone, although the hormone replacement of testosterone restored the plasma level. These results suggest that Keishi-bukuryo-gan, which does not have testosterone activity to serum, may be useful for the treatment of hot flushes due to testosterone deficiency after GnRH therapy in men with prostatic carcinoma, as well as 17β-estradiol.
4.The Current Strategy for Managing Pancreatic Neuroendocrine Tumors in Multiple Endocrine Neoplasia Type 1.
Yusuke NIINA ; Nao FUJIMORI ; Taichi NAKAMURA ; Hisato IGARASHI ; Takamasa OONO ; Kazuhiko NAKAMURA ; Masaki KATO ; Robert T JENSEN ; Tetsuhide ITO ; Ryoichi TAKAYANAGI
Gut and Liver 2012;6(3):287-294
Multiple endocrine neoplasia type 1 (MEN1) is an inherited autosomal dominant disease presenting with pancreatic neuroendocrine tumors (pNETs), parathyroid tumors, or pituitary tumors. Using the PubMed database, we reviewed the literature on information regarding the proper diagnosis and treatment of MEN1-associated pNET. Many cases of MEN1-associated pNET are functioning pNETs. Gastrinomas and insulinomas tend to occur frequently in the duodenum and pancreas, respectively. In addition to diagnostic imaging, the selective arterial secretagogue injection test (SASI test) is useful for localizing functioning pNET. The standard treatment is surgical resection. However, in the case of a functioning pNET, the tumor should first be accurately located using the SASI test before an appropriate surgical method is selected. In cases of a MEN1-associated non-functioning pNET that exceeds 2 cm in diameter, the incidence of distant metastasis is significantly increased, and surgery is recommended. In cases of unresectable pNET, a somatostatin analog has been shown to demonstrate antitumor effects and is considered to be a promising treatment. In addition, molecular-targeted drugs have recently been found to be effective in phase III clinical trials.
Diagnostic Imaging
;
Duodenum
;
Gastrinoma
;
Incidence
;
Insulinoma
;
Multiple Endocrine Neoplasia
;
Multiple Endocrine Neoplasia Type 1
;
Neoplasm Metastasis
;
Neuroectodermal Tumors, Primitive
;
Neuroendocrine Tumors
;
Pancreas
;
Pituitary Neoplasms
;
Somatostatin