HSP70 is a kind of stress protein that takes care of protein through its life, and it has attracted attention as a factor to promote health. This protein is known for its induction route through hyperthermia stimulation. We compared differences between the effects of carbonic warm water bathing and tap warm water bathing, and the report obtained interesting results. The subjects are six healthy adults (average age: 23.8±5.5 years, each three from males and females), applied full immersion bathing at 41°C for 10min in both ca. 1, 000ppm of high concentration CO₂ warm water and tap warm water, and compared HSP70 before the bathing and one day after the bathing. During the observation of 24h, external thermal stimulation such as warm bathing was banned. 3 persons took warm bathing in CO₂ water first and the other 3 persons took tap water first. There was 10 days interval between the bathing in both types of bathing.
The results showed that an increase in precordial temperature measured with a deep-body thermometer was 1.0°C in tap warm water bathing and 2.3°C in CO₂ warm water bathing. The change in HSP70 was 3.31→4.35 (AU/mg protein: p=0.08) in tap warm water bathing and 3.42→5.04 (p<0.05) in CO₂ warm water bathing. Although a slight increase was recognized in tap warm water bathing, a significant increase in HSP70 was recognized in CO₂ warm water bathing.

The purpose of this study was to determine if salivary chromogranin a secretion in dogs exhibits a circadian rhythm. Saliva sampling was performed during three different sessions occurring in three nonconsecutive 24-h periods. Sixteen healthy adult beagle dogs (8 males and 8 females) were moved to a sampling room and housed individually in cages. Saliva samples were obtained every 4 h from 12:00 p.m. to 12:00 p.m. the following day. In the interest of habituation, saliva was obtained hourly from each dog 3 h before the experiment was started. Salivary chromogranin A concentrations were measured using an enzyme-linked immunosorbent assay. No circadian rhythm was detected for salivary chromogranin A secretion, and no differences in salivary chromogranin A concentrations measured every 4 h were demonstrated during the 24-h cycle in dogs.
Animals ; Chromogranin A/*analysis/*metabolism ; *Circadian Rhythm ; Dogs/*physiology ; Saliva/*chemistry

BACKGROUND: The Hokkaido Study on Environment and Children's Health is an ongoing study consisting of two birth cohorts of different population sizes: the Sapporo cohort and the Hokkaido cohort. Our primary objectives are to (1) examine the effects that low-level environmental chemical exposures have on birth outcomes, including birth defects and growth retardation; (2) follow the development of allergies, infectious diseases, and neurobehavioral developmental disorders, as well as perform a longitudinal observation of child development; (3) identify high-risk groups based on genetic susceptibility to environmental chemicals; and (4) identify the additive effects of various chemicals, including tobacco. METHODS: The purpose of this report is to provide an update on the progress of the Hokkaido Study, summarize recent results, and suggest future directions. In particular, this report provides the latest details from questionnaire surveys, face-to-face examinations, and a collection of biological specimens from children and measurements of their chemical exposures. RESULTS: The latest findings indicate different risk factors of parental characteristics on birth outcomes and the mediating effect between socioeconomic status and children that are small for the gestational age. Maternal serum folate was not associated with birth defects. Prenatal chemical exposure and smoking were associated with birth size and growth, as well as cord blood biomarkers, such as adiponectin, leptin, thyroid, and reproductive hormones. We also found significant associations between the chemical levels and neuro development, asthma, and allergies. CONCLUSIONS Chemical exposure to children can occur both before and after birth. Longer follow-up for children is crucial in birth cohort studies to reinforce the Developmental Origins of Health and Disease hypothesis. In contrast, considering shifts in the exposure levels due to regulation is also essential, which may also change the association to health outcomes. This study found that individual susceptibility to adverse health effects depends on the genotype. Epigenome modification of DNA methylation was also discovered, indicating the necessity of examining molecular biology perspectives. International collaborations can add a new dimension to the current knowledge and provide novel discoveries in the future.
Biomarkers/blood* ; Child ; Child Health ; Child, Preschool ; Cohort Studies ; Environmental Exposure/adverse effects* ; Environmental Health ; Environmental Pollutants/adverse effects* ; Female ; Fetal Blood/chemistry* ; Follow-Up Studies ; Growth/drug effects* ; Humans ; Hypersensitivity/etiology* ; Infant ; Japan/epidemiology* ; Male ; Neurodevelopmental Disorders/etiology* ; Pregnancy ; Prenatal Exposure Delayed Effects/etiology* ; Prevalence ; Smoking/adverse effects*