1.Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation.
Luciana S ARAGÃO-FRANÇA ; Viviane C J ROCHA ; Andre CRONEMBERGER-ANDRADE ; F H B COSTA ; José Fernandes VASCONCELOS ; Daniel Abensur ATHANAZIO ; Daniela Nascimento SILVA ; E S SANTOS ; Cássio Santana MEIRA ; C F ARAÚJO ; Jéssica Vieira CERQUEIRA ; Fabíola CARDILLO ; Neuza Maria ALCÂNTARA-NEVES ; Milena Botelho Pereira SOARES ; Lain C PONTES-DE-CARVALHO
Allergy, Asthma & Immunology Research 2018;10(4):406-419
PURPOSE: The use of tolerogenic dendritic cells (TolDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of TolDC administration in a mouse model of allergic airway inflammation caused by mite extract. METHODS: Mouse bone marrow-derived TolDCs were induced by incubation with granulocyte-macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme-linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed TolDCs were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. RESULTS: Induction of TolDCs was efficiently achieved as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production, and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed TolDCs reduced the number of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, TolDC-treated mice showed lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased IFN-γ levels in the supernatant of splenocyte cultures Transplantation of TolDCs increased the percentage of the regulatory T cells in the spleen and the lungs. CONCLUSIONS: Preventive treatment with TolDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic dendritic cells for the management of allergic conditions.
Administration, Intranasal
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Animals
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Antibodies
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Antigens, Dermatophagoides
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Asthma
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Bronchoalveolar Lavage Fluid
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Dendritic Cells*
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Dexamethasone
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Dust*
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Enzyme-Linked Immunosorbent Assay
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Flow Cytometry
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Gene Expression
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Granulocyte-Macrophage Colony-Stimulating Factor
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Hypersensitivity
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Immunoglobulin E
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Immunoglobulins
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In Vitro Techniques
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Inflammation*
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Interleukin-4
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Interleukins
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Lung
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Major Histocompatibility Complex
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Mice
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Mites*
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Mucus
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Spleen
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T-Lymphocytes, Regulatory
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Up-Regulation
2.Erratum: Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation.
Luciana Souza DE ARAGÃO-FRANÇA ; Viviane Costa Junqueira ROCHA ; Andre CRONEMBERGER-ANDRADE ; Fábio Henrique Brasil DA COSTA ; Juliana Fraga VASCONCELOS ; Daniel Abensur ATHANAZIO ; Daniela Nascimento SILVA ; Emanuelle Souza SANTOS ; Cássio Santana MEIRA ; Cintia Figueiredo ARAUJO ; Jéssica Vieira CERQUEIRA ; Daniela Nascimento SILVA ; Fabíola CARDILLO ; Neuza Maria ALCÂNTARA-NEVES ; Milena Botelho Pereira SOARES ; Lain Carlos PONTES DE CARVALHO
Allergy, Asthma & Immunology Research 2018;10(6):724-725
This erratum is being published to correct the printing error on page 406 of the article. Corrections for author names are needed.
3. Chemical composition, antiparasitic and cytotoxic activities of aqueous extracts of Ziziphus joazeiro Mart.
Jacqueline ANDRADE ; Ana DA SILVA ; Antônia DOS SANTOS ; Maria FREITAS ; Yedda DE MATOS ; Maria BRAGA ; Camila BEZERRA ; Maria PEREIRA GONÇALO ; Maria VEGA GOMEZ ; Henrique MELO COUTINHO ; Jacqueline ANDRADE ; Míriam ROLÓM ; Cathia CORONEL ; Paulo VASCONCELOS RIBEIRO ; Edy DE BRITO
Asian Pacific Journal of Tropical Biomedicine 2019;9(5):222-226
To compare the in vitro antiparasitic activity of aqueous extracts from Ziziphus joazeiro leaves and stem bark against Trypanosoma cruzi, Leishmania braziliensis, and Leishmania infantum, as well as to evaluate its cytotoxicity in mammalian cells, in addition to identifying the chemical composition of the extracts. Methods: Ziziphus joazeiro leaf and stem bark aqueous extracts were prepared by cold extraction maceration and subjected to ultra-efficient liquid chromatography coupled to a quadrupole/time of flight system. The susceptibility assays used Trypanosoma cruzi CL-B5 strains and promastigote forms of Leishmania braziliensis and Leishmania infantum for antiparasitic activity of the extracts. Moreover, mammalian fibroblasts NCTC clone 929 were used for cytotoxicity analysis. Results: Terpenoid compounds, flavonoids and phenolic acid were identified in extracts. The stem bark aqueous extracts presented more significant results in terms of antiparasitic activity compared with the leaf aqueous extracts, especially against Leishmania braziliensis and Leishmania infantum promastigote forms with an IC