1. Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus
Achmad Fuad HAFID ; Adita Ayu PERMANASARI ; Myrna ADIANTI ; Lydia TUMEWU ; Aty WIDYAWARUYANTI ; Tutik Sri WAHYUNI ; Maria Inge LUSIDA ; Maria Inge SOETJIPTO ; Achmad Fuad HAFID ; Aty WIDYAWARUYANTI ; Tutik Sri WAHYUNI ; Chie AOKI-UTSUBO ; Maria Inge LUSIDA ; Sri Puji Astuti WAHYUNINGSIH ; Hak HOTTA
Asian Pacific Journal of Tropical Biomedicine 2017;7(7):633-639
Objective To determine anti-viral activities of three Artocarpus species: Artocarpus altilis, Artocarpus camansi, and Artocarpus heterophyllus (A. heterophyllus) against Hepatitis C Virus (HCV). Methods Antiviral activities of the crude extracts were examined by cell culture method using Huh7it-1 cells and HCV genotype 2a strain JFH1. The mode of action for anti-HCV activities was determined by time-of-addition experiments. The effect on HCV RNA replication and HCV accumulation in cells were analyzed by quantitative reverse transcription-PCR and western blotting, respectively. Results The dichloromethane (DCM) extract of A. heterophyllus exhibited strong anti-HCV activity with an inhibitory concentration (IC
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