1.Protective effect of lectin from Synadenium carinatum on Leishmania amazonensis infection in BALB/c mice.
Sandra R AFONSO-CARDOSO ; Flavio H RODRIGUES ; Marcio AB GOMES ; Adriano G SILVA ; Ademir ROCHA ; Aparecida HB GUIMARAES ; Ignes CANDELORO ; Silvio FAVORETO ; Marcelo S FERREIRA ; Maria A SOUZA
The Korean Journal of Parasitology 2007;45(4):255-266
The protective effect of the Synadenium carinatum latex lectin (ScLL), and the possibility of using it as an adjuvant in murine model of vaccination against American cutaneous leishmaniasis, were evaluated. BALB/c mice were immunized with the lectin ScLL (10, 50, 100 microgram/animal) separately or in association with the soluble Leishmania amazonensis antigen (SLA). After a challenge infection with 10(6) promastigotes, the injury progression was monitored weekly by measuring the footpad swelling for 10 weeks. ScLL appeared to be capable of conferring partial protection to the animals, being most evident when ScLL was used in concentrations of 50 and 100 microgram/animal. Also the parasite load in the interior of macrophages showed significant reduction (61.7%) when compared to the control group. With regard to the cellular response, ScLL 50 and 100 microgram/animal stimulated the delayed-type hypersensitivity (DTH) reaction significantly (P < 0.05) higher than SLA or SLA plus ScLL 10 weeks after the challenge infection. The detection of high levels of IgG2a and the expression of mRNA cytokines, such as IFN-gamma, IL-12, and TNF-alpha (Th1 profiles), corroborated the protective role of this lectin against cutaneous leishmaniasis. This is the first report of the ScLL effect on leishmaniasis and shows a promising role for ScLL to be explored in other experimental models for treatment of leishmaniasis.
*Adjuvants, Immunologic
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Animals
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Antibodies, Protozoan/immunology
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Antibody Formation
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Antigens, Protozoan/immunology
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Cytokines/genetics/immunology
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Euphorbiaceae/*chemistry
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Hypersensitivity, Delayed/immunology
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Immunization
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Immunoglobulin G/immunology
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Latex/chemistry
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Leishmania/immunology
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Leishmaniasis, Cutaneous/*immunology/pathology
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Mice
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Mice, Inbred BALB C
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Nitric Oxide Synthase Type II/genetics/immunology
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Plant Lectins/*immunology/isolation & purification
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Protozoan Vaccines/immunology/pharmacology
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Skin/pathology
2.Specific IgG and IgA Antibody Reactivities in Sera of Children by Enzyme-Linked Immunoassay and Comparison With Giardia duodenalis Diagnosis in Feces
Flávia Thamiris Figueiredo PACHEC ; Silvia Souza de CARVALHO ; Samara Alves SANTOS ; Gisele Maria Trindade das CHAGAS ; Mariana Conceição SANTOS ; Jéssica Gleide Souza SANTOS ; Hugo da COSTA-RIBEIRO JÚNIOR ; Tereza Cristina Medrado RIBEIRO ; Ângela Peixoto de MATTOS ; Maria Aparecida GOMES ; Neci Matos SOARES ; Márcia Cristina Aquino TEIXEIRA
Annals of Laboratory Medicine 2020;40(5):382-389
Background:
Giardia duodenalis is conventionally diagnosed in fecal samples using parasitological methods. However, sensitivity is poor when only a single sample is analyzed, due to intermittent excretion of cysts in feces. Alternatively, the serum antibodies to G. duodenalis can be used for parasite diagnosis and epidemiological studies to determine previous exposure. We compared the rate of G. duodenalis infection between serum anti-Giardia IgG and IgA antibodies and fecal examination in Brazilian children.
Methods:
Fecal and serum samples were tested from 287 children at a clinical laboratory and from 187 children at daycare centers. Fecal samples were processed using conventional parasitological methods and coproantigen detection for Giardia diagnosis. Serum samples were tested using an in-house ELISA for detection of anti-Giardia IgG and IgA.
Results:
G. duodenalis was found in 8.2% (N=39) of the 474 children analyzed. The sensitivity and specificity of ELISA were 80.0% and 90.0% for IgG and 80.0% and 83.3% for IgA, respectively. The total positivity rate of anti-Giardia IgG and IgA in the sera was 13.9% (N=66) and 23.6% (N=112). The agreement between the positivity of specific antibodies and the detection of G. duodenalis in feces was moderate for ELISA-IgG, kappa index (95% CI)=0.543 (0.422–0.664), and mild for ELISA-IgA, kappa index (95% CI)=0.283 (0.162–0.404). Among the children infected with other enteroparasites, 11.6% (N=10) and 24.4% (N=21) showed reactivity to anti-Giardia IgG and to IgA, respectively. This cross-reactivity was more frequent in samples from children infected with Endolimax nana and Entamoeba coli.
Conclusions
The higher frequency of specific antibody reactivity compared with G. duodenalis diagnosis in feces could reflect continuous exposure of children to G. duodenalis infection, resulting in long-lasting immunological memory and/or cross-reactivity with other intestinal amoebas.