1.Single fecal microbiota transplantation failed to change intestinal microbiota and had limited effectiveness against ulcerative colitis in Japanese patients.
Shinta MIZUNO ; Kosaku NANKI ; Katsuyoshi MATSUOKA ; Keiichiro SAIGUSA ; Keiko ONO ; Mari ARAI ; Shinya SUGIMOTO ; Hiroki KIYOHARA ; Moeko NAKASHIMA ; Kozue TAKESHITA ; Makoto NAGANUMA ; Wataru SUDA ; Masahira HATTORI ; Takanori KANAI
Intestinal Research 2017;15(1):68-74
BACKGROUND/AIMS: Recent developments in analytical techniques including next-generation sequencing have clarified the correlation between intestinal microbiota and inflammatory bowel disease. Fecal microbiota transplantation (FMT) for patients with ulcerative colitis (UC) is proposed as a potential approach to resolving their dysbiosis; however, its safety and efficacy have not been confirmed. This single-arm, open-label, non-randomized study aimed to evaluate the safety and efficacy of FMT for Japanese patients with UC as the first registered clinical trial in Japan. METHODS: We enrolled 10 patients with active UC despite medical therapy. The donors were the patients' relatives and were carefully screened for infectious diseases. Fecal material was administered via colonoscopy, and the primary endpoint was the presence or absence of serious adverse events related to FMT. The secondary endpoint was a change in partial Mayo score at 12 weeks post-FMT. Scores ≤2 were considered a clinical response. Fecal samples were collected to follow changes in gut microbiota, while extracted complementary DNA were analyzed by a next-generation sequencer. We obtained written informed consent from all patients and donors. This study was approved by our Institutional Review Board and is registered in the University hospital Medical Information Network (UMIN) Clinical Trials Registry (UMIN 000012814). RESULTS: Five patients with moderate disease and five with severe disease were enrolled. No severe adverse effects were observed. One patient achieved clinical response; however, none of the patients' microbiota diversity recovered to the donor levels. CONCLUSIONS: The use of single FMT for UC was safe; however, we failed to show its clinical efficacy and potential to change the intestinal microbiota.
Asian Continental Ancestry Group*
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Colitis, Ulcerative*
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Colonoscopy
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Communicable Diseases
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DNA, Complementary
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Dysbiosis
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Ethics Committees, Research
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Fecal Microbiota Transplantation*
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Gastrointestinal Microbiome*
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Humans
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Inflammatory Bowel Diseases
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Information Services
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Informed Consent
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Japan
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Microbiota
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Tissue Donors
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Treatment Outcome
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Ulcer*
2.Fecal microbiota transplantation for recurrent Clostridium difficile infection in a patient with ulcerative colitis
Kosaku NANKI ; Shinta MIZUNO ; Katsuyoshi MATSUOKA ; Keiko ONO ; Shinya SUGIMOTO ; Hiroki KIYOHARA ; Mari ARAI ; Moeko NAKASHIMA ; Kozue TAKESHITA ; Keiichiro SAIGUSA ; Mitsutoshi SENOH ; Tadashi FUKUDA ; Makoto NAGANUMA ; Haru KATO ; Wataru SUDA ; Masahira HATTORI ; Takanori KANAI
Intestinal Research 2018;16(1):142-146
Fecal microbiota transplantation (FMT) has been reported as a safe and effective therapy in patients with refractory and recurrent Clostridium difficile infection (CDI). FMT has also been reported as a promising therapy in patients with ulcerative colitis (UC). Both, CDI and UC, are believed to be caused by dysbiosis, such as altered compositions or decreased diversity of the intestinal microbiota. This report describes a patient with UC in remission with a second recurrent episode of CDI, who was treated with FMT. A single FMT performed via colonoscopy completely resolved the patient's diarrhea and eradicated C. difficile bacteriologically without any severe complications. Molecular biological analysis of the patient's fecal microbiota showed that FMT could dramatically change the altered composition of intestinal microbiota and restore its diversity. Despite the restoration of the intestinal microbiota, FMT could not prevent a relapse of UC in this patient. However, it improved the intestinal symptoms of CDI and could prevent further recurrences of CDI.
Clostridium difficile
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Clostridium
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Colitis, Ulcerative
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Colonoscopy
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Diarrhea
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Dysbiosis
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Fecal Microbiota Transplantation
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Gastrointestinal Microbiome
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Humans
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Microbiota
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Recurrence
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Ulcer
3.A Booklet for Families of Children Dying with Incurable Cancer: Development and Feasibility Study by Opinions of Pediatric Oncology Specialists
Wataru IRIE ; Yuko NAGOYA ; Yuko HATORI ; Saran YOSHIDA ; Akiko OGATA ; Mari MATSUOKA ; Ryohei TATARA ; Jun NAGAYAMA ; Mitsunori MIYASHITA ; Hitoshi SHIWAKU
Palliative Care Research 2018;13(4):383-391
The purpose of this study was to clarify feasibility of a booklet for families of children dying with incurable cancer. Of 267 pediatric oncology specialists, 76 (28%) participated in this cross-sectional survey. Ninety-three percent of participants answered that they were “likely to use” the booklet, and 86% percent answered that they believed the booklet was “helpful” for families. Some participants described various advantages of its use as follows: “able to communicate something that is difficult to put into words,” “easy to understand for families,” “families can read whenever they choose to,” and “able to grasp the direction of dying.” In contrast, other participants described points of attention of use as follows: “optimal timing to bring out,” “acceptance of families,” “direction as interdisciplinary team,” “confidential relationship between families and interdisciplinary team,” “disinclination among health care providers,” and “information volume of the booklet.” Thus, our results validated feasibility of the booklet. In conclusion, pediatric oncology specialists should provide their support of utilizing the booklet for families and assessing each family condition and advantage/attention of using the booklet.