1.Apaf1 inhibition promotes cell recovery from apoptosis.
Anna GORTAT ; Mónica SANCHO ; Laura MONDRAGÓN ; Àngel MESSEGUER ; Enrique PÉREZ-PAYÁ ; Mar ORZÁEZ
Protein & Cell 2015;6(11):833-843
The protein apoptotic protease activating factor 1 (Apaf1) is the central component of the apoptosome, a multiprotein complex that activates procaspase-9 after cytochrome c release from the mitochondria in the intrinsic pathway of apoptosis. We have developed a vital method that allows fluorescence-activated cell sorting of cells at different stages of the apoptotic pathway and demonstrated that upon pharmacological inhibition of Apaf1, cells recover from doxorubicin- or hypoxia-induced early apoptosis to normal healthy cell. Inhibiting Apaf1 not only prevents procaspase-9 activation but delays massive mitochondrial damage allowing cell recovery.
Adenosine Triphosphate
;
metabolism
;
Apoptosis
;
drug effects
;
Apoptotic Protease-Activating Factor 1
;
genetics
;
metabolism
;
Cell Hypoxia
;
drug effects
;
Cell Line, Tumor
;
Doxorubicin
;
pharmacology
;
HeLa Cells
;
Humans
;
Microscopy, Electron, Transmission