1.Relationship of Resistance to Trimethoprim-sulfamethoxazole and Genes sul1 and sul2 in Stenotrophomonas maltophilia
Jinwei HUANG ; Suwei CHEN ; Jiansheng HUANG ; Maowen DING ; Lizhu CHEN ; Guoxiong LI
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To detect genes sul1 and sul2 in Stenotrophomonas maltophilia,and their relationship to drug resistance to trimethoprim-sulfamethoxazole(SXT).METHODS K-B was carried out to detect the drug resistance to SXT of S.maltophilia;minimal inhibitory concentration(MIC) was measured with micro broth dilution method.Genes sul1 and sul2 were amplified by PCR.RESULTS Eight isolates(7.8%) showed resistance to trimethoprim-sulfamethoxazole,sul1 Was positive in four isolates in which one contained sul2 also.Four isolates showed high MIC to SXT.CONCLUSIONS Genes sul1 and sul2 of S.maltophilia are associated with the high drug resistance to SXT.
2.The status of diagnosis and treatment on invasive fungal pulmonary infections
Jinwei HUANG ; Haiquan CHEN ; Fangyou YU ; Zhongliang YE ; Lifen CHEN ; Maowen DING ; Guoxiong LI
Chinese Journal of Clinical Infectious Diseases 2009;2(3):151-153
Objective To review the status of diagnosis and treatment for invasive fungal pulmonary infections(IFPI)in Lishui Central Hospital.Methods The clinical data of 79 patients with IFPI were retrospectively analyzed.Results The diagnostic status could be classified ills follows:6 eases had confirmed diagnosis,30 had clinical diagnosis,35 had suspected diagnosis and 8 misdiagnosed.The treatments were all effeetive in 6 COnfirmed cases;in 30 clinically diagnosed cases,6 were eriective.21 were inefiective and 3 died;in 35 suspected cases.3 were effective.25 were iHefieetive and 7 cflses did not receive antifungal treatment.Aspergillus and Cryptococcus pulmonary infections were predominant in confirmed cases.and the antifungal treatment lasted for 3 to 6 months.Conclusion Diagnosis and treatment for IFPI need to be improved.
3.Etiological identification and phylogenetic analysis of a clustered epidemic caused by norovirus on a patrol boat
Jiwei SHU ; Linfu GUAN ; Tongjie ZHANG ; Yi REN ; Ling YE ; Ni XU ; Rong WANG ; Maowen HUANG
Shanghai Journal of Preventive Medicine 2022;34(11):1112-1117
ObjectiveTo determine the pathogen and phylogenetic characteristics of an uncommon outbreak of recombinant norovirus infection in Daishan County in February 2022. MethodsFluorescence quantitative PCR was used to detect the norovirus in the eight anal swabs collected in the outbreak. In the positive samples, reverse transcription PCR were used to amplify the norovirus. Norovirus sequences were characterized by MEGA7 and Simplot. ResultsNorovirus GⅠ was identified in all eight anal samples. It was further determined to be recombinant norovirus GⅠ.6 [P11], with the recombination site at the ORF1-ORF2 junction. The sequence had the highest nucleotide identity (98.75%) to a GⅠ.6[P11] strain collected in 2018 (GenBank accession number MT357995). ConclusionAccording to the etiological identification and phylogenetic analysis, this outbreak is confirmed to be caused by the uncommon recombinant norovirus GⅠ.6 [P11] in China.