Objective To identify the relationship between among TCM constitutions, related genes and risk factors of gastric cancer.Methods A total of 91 healthy population were recruided, among whom 29 were Qi-deficiency, 23 were Yang-deficiency, 26 were Wetness-heat type and 13 were Gentleness type. The dietary habit, family cancer history and Helicobacter pylori infection was selected as the risk factor for gastric cancer. TheTP53RS1042522 and IL1BRS1143634 were detected by the technology of high throughput gene microarray in our study. Statistical analyses were carried out with SPSS21.0 for windows.Results There was no significant difference in the dietary habit and family cancer history among four groups. The population with Helicobacter pylori infection showed high propotion in the group of wetness-heat type constitution (32.56%, P<0.05). The frequency of G allele of TP53RS1042522 in the groups of four TCM constitutions were 75.86%, 82.61%, 84.62%, 53.85%. The group of wetness-heat type constitution showed significantly more related with the polymorphism than the group of Gentleness type constitution (OR=0.212, 95%CI: 0.046-0.974,P=0.046). The frequency of C allele of IL1BRS1143634 the groups of four TCM constitutions were 75.86%, 82.61%, 76.92%, 69.23%. This polymorphism was not statistically significant associated with any constitutions. ConclusionWetness-heat type constitution showed high correlation with Helicobacter pylori infection and G allele of TP53RS1042522.