Objectives Research into effect on PBL model in prosthodontics education.Methods The PBL model was performed in education of a part of prosthodontics,and education effect was evaluated by table analysis.Results PBL model is proved to be better than the multimedia education model in the training of students'self-study ability,learning initiative,problem-analyzing and problem-solving competence,and the team spirit etc.Conclution Comparaed with the multimedia education model,PBL model is of significance in some respects.

OBJECTIVETo study therapeutic effects of patella and femur fixation combined with percutaneous suture in the treatment of patellar fractures.

METHODSIn the study,23 patients with patellar fractures were treated with percutaneous Kirschner-pin transfixation and percutaneous suture after Kirschner pins transfixation of superior pole of patellar and condyle of femur. Among the patients, 17 patients were male and 6 patients were female, ranging in age from 18 to 73 years (mean 32.4 years). The therapeutic effects were evaluated according to Bistman criterion for patellar fracture.

RESULTSAll the patients were followed up and ranged from 6 to 38 months (mean 11 months). All the fractures were anatomically reduction or near-anatomical reduction. According to Böstman criterion for patellar fracture, 20 were excellent and 3 good.

CONCLUSIONThe method of patella and femur fixation combined with suture for patellar fractures has such advantages as smaller invasion, less complication or sequel, fine reduction of fracture, good restoration of joint function and none operative cicatrix.

Adult ; Aged ; Female ; Fracture Fixation, Internal ; Fractures, Bone ; surgery ; Humans ; Male ; Middle Aged ; Patella ; injuries ; surgery ; Suture Techniques ; Treatment Outcome

Adolescent ; Adult ; Antiviral Agents ; therapeutic use ; Female ; Hepatitis B, Chronic ; drug therapy ; immunology ; Humans ; Lamivudine ; therapeutic use ; Male ; Th1 Cells ; immunology ; Th2 Cells ; immunology

This study sought to explore the relationship between the change in ventricular electrical remodeling caused by mechano-electrical feedback and the expression of L-type Ca2+ -channel and/or sarcoplasmic reticulum Ca2+ -ATPase in the rabbits with congestive heart failure (CHF). 138 rabbits were divided into two groups (CHF and control). We measured the ventricular monophasic action potential duration (MAPD) and ventricular effective refractory period (VERP) during ventricular pacing at the stimulus frequency of 220/240/260 bpm in these rabbits. Rapid atrial pacing (260/min) was given for 30 minutes. The MAPD and VERP were measured again. Then ventricular fibrillation was induced by S1S2S3 program stimulation. We extracted the total RNA from the myocardium respectively and detected L-type Ca2+ -channel mRNA and sarcoplasmic reticulum Ca2+ -ATPase mRNA by use of Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). In group CHF, with the increasing of preload/afterload, L-type Ca2+ -channel mRNA was up regulated after rapid atrial pacing when compared with that in control groups (P < 0.05). There was no significant change in sarcoplasmic reticulum Ca2+ -ATPase mRNA after rapid atrial pacing when compared with controls (P > or = 0.05). The changes in MAPD90 and VERP were related with the extent of L-type Ca2+ -channel mRNA up regulation. But the changes in MAPD90 and VERP were not significantly related with the extent of sarcoplasmic reticulum Ca2+ -ATPase mRNA up regulation. These findings suggest that Mechano-Electrical Feedback could increase the regional changes of ventricular electrical remodeling in rabbits with CHF and so to predispose them to ventricular arrhythmia. The changes may be related with the up regulation of L-type Ca2+ -channel mRNA, but not with sarcoplasmic reticulum Ca2+ -ATPase mRNA.
Action Potentials ; Animals ; Calcium Channels, L-Type ; genetics ; metabolism ; Cardiac Pacing, Artificial ; Electric Conductivity ; Electrophysiology ; Female ; Heart Failure ; metabolism ; physiopathology ; Male ; RNA, Messenger ; genetics ; metabolism ; Rabbits ; Random Allocation ; Refractory Period, Electrophysiological ; Sarcoplasmic Reticulum ; genetics ; metabolism ; Ventricular Remodeling

Adolescent ; Adult ; Antiviral Agents ; therapeutic use ; Female ; Hepatitis B virus ; drug effects ; genetics ; Hepatitis B, Chronic ; blood ; drug therapy ; immunology ; Humans ; Interleukin-12 ; blood ; Lamivudine ; therapeutic use ; Male ; Mutation

OBJECTIVETo investigate whether human L02 hepatocytes could survive after implanting them into normal, immunocompetent rats.

METHODSHuman L02 hepatocytes were injected through the uterine walls into the intraperitoneal cavities of fetal Sprague-Dawley rats to induce immune tolerance to human L02 hepatocytes. Human L02 hepatocytes stained with DiI were implanted into the spleens of the 2-week old rats. Immuno-fluorescent staining, SP immunohistochemistry, and DiI staining were used to detect human albumin and specific proliferating cell nuclear antigen (PCNA) in the rat livers. The distribution of human L02 hepatocytes was observed under the fluorescent microscope.

RESULTSDynamic distribution of human L02 hepatocytes in the rat livers was observed from the 1st to the 10th week after the implantation. Human albumin was detected at 2, 4, 6 and 8 weeks, and at the 4th week it had the highest level. Specific human PCNA was detected in the rat livers from the 2nd to the 6th week after implantation. The PCNA positive cells were most abundant at the 4th week.

CONCLUSIONHuman L02 hepatocytes can survive and proliferate for 10 weeks after implanting them into genetically normal immunocompetent rats.

Animals ; Cell Transplantation ; Cells, Cultured ; Disease Models, Animal ; Female ; Graft Survival ; Hepatocytes ; cytology ; Humans ; Pregnancy ; Rats ; Rats, Sprague-Dawley

To assess the changes of sarcolemma Na+/K+ ATPase (CMNKA) and sarcoplasmic reticulum membrane Ca2+ ATPase (SERCA) activities after stem cells transplantation in heart failure. Rabbit was used as heart failure model by intravenously injecting adriamycin. Autologous bone marrow mononuclear cells (BMCs), bone marrow mesenchymal stem cells (MSCs) or skeletal myoblasts (SMs) were introduced into coronary arteies through the root of aorta when two balloons occluding just above sinus of Valsalva. After 4 weeks, left ventricular ejection fraction (LVEF)was evaluated by echocardiography, and the activities of CMNKA and SERCA were measured by colorimeter. In BMCs (n=8)and MSCs (n=8) group, LVEF were significantly improved (P < 0.05). No significant improvement were seen in SMs group (n=6) compared to sham group (n=8). The CMNKA activity in all stem cells groups was significantly increased compared to sham group (P < 0.05). Meanwhile, in comparison with sham group, the incremental tendencies of SERCA activity were seen in stem cells groups. In conclusion, stem cells transplantation could increase the activities of CMNKA and SERCA in heart failure, a possible mechanism to improve heart function.
Animals ; Doxorubicin ; Female ; Heart Failure ; chemically induced ; enzymology ; therapy ; Male ; Myocardium ; enzymology ; Rabbits ; Random Allocation ; Sarcolemma ; enzymology ; Sarcoplasmic Reticulum Calcium-Transporting ATPases ; metabolism ; Sodium-Potassium-Exchanging ATPase ; metabolism ; Stem Cell Transplantation

To investigate the feasibility of introcoronary cell infusion into nonischemic heart failure (HF) heart and whether different types of stem cell transplantation would affect heart function to a similar degree. Japanese white ears rabbits were used as HF models by intravenous injection adriamycin. Autologous bone marrow mononuclear cells(BMCs), bone marrow stromal cells (MSCs), skeletal myoblasts (SMs) or culture medium were infused into coronary arteries respectively by occluding the root of ascending aorta. The mortality during and 4 weeks after the procedure the mortality was 7.1% and 16.7% respectively. After 4 weeks, the ejection fraction (EF) in BMCs group had significant improvement (P < 0.05, n=8). No significant difference was seen in MSCs (n =8), SMs (n=6) and sham groups (n=8) compared with pretransplantation (P > 0.05). In sham group,the left ventricular endostolic diameter (LVED) had significant enlargement (P < 0.05), No significant difference was seen in MBCs, MSCs and SMs groups compared with pretransplantation (P > 0.05). Immunofluorescence revealed de novo expression of cardiac troponin I in BMCs and MSCs groups, cardiac troponin I was not detected in SMs group. In conclusions, intracoronary cell transplantation could provide effective cell delivery into dilated cardiomyopathy hearts and could be a useful strategy for treating CHF, BMCs cell transplantation may be the first choice in all the above cell types.
Animals ; Bone Marrow Transplantation ; Chronic Disease ; Coronary Vessels ; Heart Failure ; physiopathology ; surgery ; Infusions, Intra-Arterial ; Mesenchymal Stem Cell Transplantation ; Myoblasts, Skeletal ; transplantation ; Rabbits ; Random Allocation ; Stem Cell Transplantation ; methods ; Troponin ; metabolism ; Ventricular Function, Left ; physiology

[Objective]To study the change of amplitude-integrated electroencephalography(aEEG)and the relationship with neurological prognosis in children with brain injury.[Methods]This study included 100 brain injured children,100 normal children as the control group. Quantitative aEEG in frontal,temporal,parietal and central lobe were compared. The aEEG in brain injured chil?dren were reexamined after 1 week. The follow-up was observed after 3 months and pediatric cerebral performance category score (PCPC)was recorded for each patient.[Results]The 2-12 months group frontal and temporal lower margin amplitude were signifi?cantly increased,P<0.05,bandwidth in frontal,temporal and central channels were significantly decreased,P<0.05. In 13-35 months group all lower and upper margin amplitudes were significantly higher in brain injury group than normal control group ,P<0.05. In 36-71 months group,frontal,temporal,parietal and central lower margin amplitude were significantly increased,P<0.05. However,only upper margin amplitude in frontal and parietal lobe were significantly different,P<0.05. In 72-155 months group,the lower margin amplitude of frontal,central,parietal and temporal channels were significantly increased,P<0.05;the upper margin amplitude of frontal,central,parietal channels were significantly different,P<0.05;the bandwidth were significantly different in fron? tal and parietal channels,P<0.05. In 156-216 months group,the lower and upper margin amplitude of frontal,central,parietal and temporal channels were significantly increased ,P<0.05;the bandwidth were significantly different in frontal channel ,P<0.05.Correla?tion analysis shows that the PCPC after 3 months was significantly correlated to upper and lower voltage of parietal channels,r=0.222, 0.219,respectively.[Conclusion]The lower margin amplitude in brain injured children were changed,and the early aEEG of pari?etal channel have predict value for early prognosis.

OBJECTIVETo assess the value of three dimensional proton magnetic resonance spectroscopy (3D 1H-MRS) with body coil at 3T in the differential diagnosis of prostate cancer.

METHODSForty patients suspected of prostate cancer underwent MRI and MRS examinations, and then transrectal ultrasound guided prostate biopsy for pathological diagnosis. The MRI and MRS features of benign prostate hyperplasia, prostate cancer and prostatic intraepithelial neoplasia (PIN) were analyzed in comparison with the pathological reports, and the receiver operating characteristic curve was drawn for the diagnosis of cancer from peripheral zones.

RESULTSThe examinations were accomplished for all the patients. The mean ratios of (Cho + Cre)/Cit in the interstitial and glandular hyperplasia tissues, the cancer tissue of the central and peripheral glands, the healthy peripheral gland and PIN were 0.75 +/- 0.23, 0.59 +/- 0.14, 1.79 +/- 0.90, 1.18 +/- 0.95, 0.46 +/- 0.18, and 0.97 +/- 0.10, respectively, with statistically significant differences between the cancer and normal prostate tissues (P < 0.01). The optimum threshold for the diagnosis of prostate cancer in the peripheral zone was 0.68 with a sensitivity of 88.6% and a specificity of 88.7%.

CONCLUSIONThe 3D 1H-MRS with body coil at 3T has a high sensitivity and specificity in the differential diagnosis of prostate cancer, and can provide valuable information for the diagnosis of PIN.

Aged ; Aged, 80 and over ; Diagnosis, Differential ; Humans ; Imaging, Three-Dimensional ; Magnetic Resonance Spectroscopy ; methods ; Male ; Middle Aged ; Prostatic Neoplasms ; diagnosis ; Sensitivity and Specificity