1.Repair of bone defect with chitin/rhBMP2/collagen complex
Fulin CHEN ; Tianqiu MAO ; Changyong WANG ; Ming ZHAO ; Huixin WANG
Journal of Practical Stomatology 1995;0(04):-
Objective: To investigate the feasibility of chitin as bone substitute material and carrier of rhBMP2.Methods: Porous chitin and chitin/rhBMP2/collagen complex were implanted into calvarial defects in 8 rabbits. Bone repairing ability was assessed by radiographic and histological observation. 2 rabbits without implantation were served as controls. Results: Chitin had certain bone conductive ability. When combined with rhBMP2,a complex possessing both bone conductive activity and bone inductive activity was produced. The complex had greater bone repairing ability than chitin alone. Conclusion: Chitin may be used as a bone substitute material and carrier of BMP. But its mechanical strength and surface activity should be improved.
3.Field Investigation of Sparganosis in Frogs in Huaxi Area
Jiazhi MAO ; Bijun ZHOU ; Kaigong WANG ; Ming WEN
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(02):-
Frogs were caught from 4 towns in Huaxi of Guiyang and dissected.The collected spargana were used to infect young dogs for species identification.Results showed that the wild frogs were identified as Rana nigromaculata, and the infection rate was 16%(131/818) with an average intensity infection of 3.44 per frog, The tapeworm obtained from an infected dog was specified as Spirometra mansoni.
4.Study on Mechanism and Regulation of CD3AK Cytotoxic Activity
Yongzhong LIU ; Ling ZHANG ; Yun WANG ; Ming GUO ; Haiting MAO ;
Chinese Journal of Cancer Biotherapy 1996;0(04):-
Human CD3AK cells were prepared from peripheral blood mononuclear cells by culturing with recombinant IL-2 and antiCD3AK McAb. The mechanism and regulation of CD3AK cytotoxic activity with cytokines (rhIFN-?, rhIFN-?, TNF) and chernotherapeutic agents (CDDP or ADM) were observed by LDH-release assay, ABC-CELISA and the flow cytometric assay. The results showed: (1) Adhesion molecules ICAM-l/LFA-1 participated in CD3AK-mediated killing of tumor cells, hrlFN-? and TNF enhanced cytotoxicity of CD3AK through this pathway. (2) CD3AK could indirectly kill tumor cells by releasing soluable cytotoxic factors. (3) The membrane-associated TNF may be involved in CD3AK-mediated cytotoxicity. (4) CD3AK cells could induce the apoptosis of tumor cells. (5) Pretreatment of tumor cells with CDDP or ADM resulted in the increased vulnerability of tumor cells to CD3AK-mediated killing, the enhancement of CD3AK-mediated cytotoxicity by CDDP was relative to the increased expression of ICAM-1, HLA-ABC on tumor cell membrane.
5.Surveys on iodine nutritional status of pregnant and lactating women in coastal areas of Zhejiang province
Xiao-ming, LOU ; Zhe, MO ; Gang-qiang, DING ; Wen-ming, ZHU ; Guang-ming, MAO ; Jin-shui, ZHOU ; Xiao-feng, WANG
Chinese Journal of Endemiology 2011;30(6):602-605
Objective To evaluate the nutritional status of iodine of pregnant and lactating women in coastal and inland areas of Zhejiang province,and to provide scientific basis for iodine supplementation.Methods In 2009,five coastal cities,five coastal counties and three inland areas from Zhejiang province were selected by the cluster sampling method,and one sub-district(township) was randomly selected in each city (county),then one community (village) was randomly selected in each sub-district (township).Thirty pregnant women,thirty lactating women and fifty children aged 8 - 10,were randomly selected in each community(village) as the investigation objects to collect instant urine samples to detect urine iodine,and to collect thirty resident edible salt and some water samples to detect salt iodine and water iodine,if the numbers of water source were more than 5 then 1 water sample was collected by their location of east,south,west,north and center in each community(village),otherwise,the numbers of water samples collected were equal to the number of community (village).Results The medians of salt iodine of coastal city,coastal county and inland area were 28.42,25.29,and 33.24 mg/kg,respectively,and the medians of water iodine correspondingly were 5.41,11.52,and 2.80 μg/L,respectively.The medians of urinary iodine of pregnant and lactating women in Zhejiang province were 148.72 and 161.34 μg/L,respectively,which were less than that of children aged 8 - 10(231.02 μg/L,Z =8.57,8.24,all P < 0.017).The medians of urinary iodine in pregnant women from different areas were ranked in order as inland areas(211.42 μg/L),coastal counties (150.51 μg/L) and coastal cities (123.33 μg/L,Z =2.80,5.31,2.42,all P < 0.017),and in lactating women,the medians of urinary iodine in inland areas( 197.43 μg/L) was higher than that in coastal cities( 139.64 μg/L,Z =4.03,P < 0.017).Conclusions The overall levels of iodine nutrition in lactating and pregnant women in Zhejiang province are in the appropriate level,but that of the pregnant women in the coastal cities is inadequate.
6.Pathological Basis of Ovarian Cystic Teratoma and its Differential Diagnosis Using Grey Scale Ultrasonography
Qingwei DING ; Ning SUN ; Shaowen WANG ; Guoying SUN ; Huifang MAO ; Zhengtao ZHU ; Ming LI ; Meiyu WANG
Chinese Journal of Medical Imaging 2010;(1):69-71,74
Purpose To investigate the diagnostic value of gray-scale ultrasound on ovarian cystic teratoma.Materials and Methods 83 patients with primary diagnosis of ovarian cystic teratoma underwent gray-scale ultrasonography. The ultrasonic images were compared with postoperative pathologic results. Results 91 cases of 83 lesions were detected. The ultrasonic features were categorized into 6 types: cystoid, solid-like, intracystic dough sign, intracystic hair-ball, intracytic fat-fluid level, and intracytic cluttering sign. The diagnose accordance rate for each sign was 81.0%, 77.8%, 96.2%, 100% and 70.0% respectively. The diagnose accordance rate of ultrasonic diagnosis compared with pathologic results was 89.0% (81/91), and misdiagnosed rate and rate of missing diagnosis was 11.0% (10/91). Conclusion The ultrasonic features of ovarian cystic mature terotoma were of importance diagnostic value, whereas differentiation with ovarian chocolate cysts and ovarian mucus-cystic adenoma should be made cautiously.
7.Perspective and application of metabonomics in modern study of traditional Chinese medicine.
Kun-Ming QIN ; Bin WANG ; Lin-Wei CHEN ; Mao-Sen ZHANG ; Guang-Ming YANG ; Ya-Chun SHU ; Bao-Chang CAI
China Journal of Chinese Materia Medica 2014;39(16):3010-3017
Metabonomics is a new method to study on the metabolic network and the relationship between body and environment, which conforms to the way of traditional Chinese medicine (TCM) research. In the study process of modernization of traditional Chinese medicine, effectively conjunction with metabonomics method will facilitate the integration of TCM with modern biological science and technology, and promote the modernization of TCM. This paper introduce the application of metabonomics in the research of toxicity mechanism of TCM, compatibility mechanism of TCM formula, pharmacology effect of TCM and processing mechanism of TCM. This paper summarize the problems in the TCM metabonomics research and prospect its bright future.
Animals
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Drug Therapy
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Drugs, Chinese Herbal
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adverse effects
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analysis
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metabolism
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therapeutic use
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Humans
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Medicine, Chinese Traditional
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methods
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trends
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Metabolomics
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methods
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trends
8.Antisense Sites Screening of Fas Gene mRNA and Its Validation in vitro
Gang ZUO ; Hui-Ming HAN ; Xiao-Li TIAN ; Quan-Hui WANG ; Jian-Ping MAO ;
China Biotechnology 2006;0(04):-
Three candidate antisense target sites of mouse Fas gene were screened by PARASS (poly-A anchored RNA accessible sites screening) technology. They were target at Fas gene 297nt-317nt, 618nt- 638nt and 662nt-682nt. Antisense oligos (A1, A2 and A3) and DNAzymes (D1, D2, and D3) for every target site were designed and synthesized. In vitro, the validation of the sites were judged by antisense oligos included RNase H splicing and the DNAzyme degradation. The results indicated that A1, A2 and A3 introduced RNase H degradation. DNAzymes D1, D2 and D3 cleaved Fas mRNA effectively. Neither degradation observed in antisense oligo RNase H group in non-target site (1211-1231nt) and 2 bases mismatched of A3, nor splicing occurred in DNzyme group in non-target site ( 1211-1231nt) and 2 bases mismatched of D3. Site 2 and 3 were at the same positions with those of ISIS Pharmaceuticals. The effective antisense oligos and DNAzymes for Fas gene could be used for the research subsequently.
9.The clinical analysis of 260 sporadic hepatitis E
Guang-Ming XIAO ; Mao-Sheng WU ; Kai-Yin HE ; Yuan-Huang LIU ; Jian-Hua WANG ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(09):-
0.05), but the level of albumin dropped significantly(P
10.Design and simulation of interference current therapeutic instruments based on FPGA.
Qian WANG ; Zhe-Min DUAN ; De-Ming MAO
Chinese Journal of Medical Instrumentation 2008;32(2):100-104
This paper applies the DDFS theory, uses the method based on the Look-Up-Table, and designs an interference current therapeutic instrument with the automatic frequency scanning module and DDFS module to generate two different interference currents. The design of every module and the whole system are simulated by quartus II, the result shows that the design principle is correct and feasible, and worthy of applications.
Electric Stimulation Therapy
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instrumentation
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Equipment Design
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Signal Processing, Computer-Assisted
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instrumentation