Objective] The two-step massage treatment of tennis elbow and simple massage therapy clinical curative effect around the elbow. [Method] A total of 80 cases of external humeral epicondylitis were randomly divided into the treatment group(42 cases) and control group(38 cases). The treatment group were treated with the combination of the neck and elbows. The control group was treated only with simple elbow massage. [Results] The treatment group cure rate was 71.4%, the total efficiency of 92.9%;control group, the cure rate was 34.2%, the total effective rate was 71%. The comparison between the 2 groups, the cure rate and total effective rate differences were significant( P＜0.01, P＜0.05). [Conclusion]Two-step massage has better cure effect on external humeral cpicondylitis.

Objective The purpose of this study was to develop a new cultural method, so that the rat bone marrow stromal cells (MSCs) can well differentiate into osteoblasts in vitro and the cultured osteoblasts can be identified. Methods MSCs of rats were isolated and cultured, then the cultured cells were identified with inverted microscopy and undergone an HE stain for observing the morphology of living cells, and the cells were histochemically stained for examining the activity of cellular alkaline phosphatase (ALP), as well as were radio-immunologically analyzed for detecting the secretion of osteocalcin. Results The MSCs were typical fibroblast-like and possess a strang proliferative capability after cultured in vitro.The cellular ALP activity and osteocalcin secretion achieved the peak value from the fortieth day and maintained the status until the sixtieth day after cultured in vitro.Conclusion The data demonstrated that the new developed culture method has the advantages of short time, less risk of contamination and higher efficiency, and conducive to MSC's differentiating and proliferating into osteoblasts that do function well.

Objective To study MRI characteristics and value in the diagnosis of nasopharyngeal carcinoma with skull base erosion. Methods 86 cases of nasopharygeal carcinoma with positive nasopharyngeal pathology and symptoms of skull base erosion were selected. Axial T1WI, T2WI and coronal, sagital T1WI were performed. 47 cases had axial, coronal and sagital T1WI examination post Gd-DTPA injection. Results Areas of skull base involvement were as follow: 56 cases with pterygoid process, 54 with clivus, 28 with foramen ovale (including sphenoid wing), 27 with petrous apex, 1 with occiptal bone. 71 cases had more than one area involvement. There are four patterns in our group: ①Defect in the signal avoid cortex of skull base; ②High signal intensity of marrow was replaced by tumor; ③Contrast enhancement in abnormal bone; ④String like enhancement passing nerve foramen in skull base. 44 cases included intracranial abnormal. Conclusion MRI is the preferred technique in dementstrating skull base involvemetn of nasopharyngeal carcinoman.

Rhizome anemarrhena,sweet and bitter to taste,cold,in the lung,stomach and kidney meridian,has the function clearing away heat and purging pathogenic fire,nourishing Yin and moistening dryness.Zhang Zhong Jin in "Essential Prescriptions of the Golden Chamber "with a different combination of herbs and Rhizome anemarrhenae,reflects the multiple efficacies of TCM and compatibility.

There are so many patients with different liver diseases in our country that the diagnosis and treatment of liver diseases directly relate to the people's healthy level.The requirement of diagnosis and treatment to clinical laboratories can be summarized as: ( 1 ) sensitivity,which means shortening window period;(2) specificity,which means various subtypes and variant can be detected;( 3 )speed,which means finding out the pathogen as soon as possible;(4)accuracy,which means providing physicians with accurate test reports.Detecting techniques,for instances,chemiluminescence,polymerase chain reaction and gene mutational site detecting method,were applied to the diagnosis and treatment of liver disease,thus improving the degree of precision,accuracy,sensitivity,specificity and detection speed and satisfying the requirement of diagnosis and treatment maximatily.

Recently, heme oxygenase-1 (HO-1) has been emphasized in the area of intensive care unit (ICU) and other domains. HO-1 can catalyze the decomposition of heine and the products of its enzymatic activity, including carbon monoxide, biliverdin, and bilirubin and ferritin can play a significant cytoprotec-tive role in antagonizing inflammation, protecting cells from oxidative injury and cellular stresses. In the re-search of severe acute pancreatitis (SAP), researchers have realized that it is of great importance to antago-nize the overflow of cytokines in order to improve the therapeutic effect of SAP. In this article, we give a re-view of the research progress of HO-1 and its relationship with SAP.

Objective To evaluate the impact of warmed and humidified CO2 on the gynecological laparoscopic operation. Methods We searched keywords related to gynecological laparoscopic and CO2 pneumoperitoneum temperature and humidity on Pubmed and Wanfang database, then analyze the postoperative pain, analgesic consumption, core temperature, operation time, length of hospital stay. Results 17 publications included into the meta analysis after screening. Compared with the standard laparoscopy, warmed and humidified CO2 can reduce postoperative pain and prevent intraoperative hypothermia. No significant difference in postoperative analgesic consumption, postoperative core temperature, duration of recovery and hospital stay, endoscope lens fog and operation time. Conclusions Only few beneficial effects on postoperative pain and core temperature have been identified in this meta-analysis, though more studies are probably needed to study the real impact of warmed and humidified CO2 for laparoscopic procedures.

The effects of carbamates and soman on the metabolism of nicotinic acetylcholine receptors (N-AChR) in cultured skeletal muscle cells were studied by 125I-a-bungarotoxin (125I-a-BTX), a specific marker. It was indicated that N~AChR degradation process was inhibited and the incorperation rate of IshAChR and the number of N~AChR in the surface of the cell membrane were increased 4h after prertreatment of high concentrations of carbamates. The number of N-AChR was also increased after pre-treatment of low concentrations of soman, whose effects on N-AChR metabolism were similar to those of high concentrations of carbamates. The incorperation rate of N-AChR was observed by inhibiting the protein synthesis with puromysin, suggesting that soman may increase the number of N-AChR by increasing the incorperation of N-AChR instead of increasing the synthesis of N-AChR.

Objective To observe the changes of serum interleukin and T lymphocyte subsets levels in patients with gallstones before and after treatment. Methods 72 patients with gallstones were treated as research subjects and 30 cases with normal physical examination as the control group,the serum interleukin and T lymphocyte subsets levels of observation group were detected before and after surgery. Results The preoperative serum IL-6, IL-8 and TNF-α levels of observation group were(61.5 ±4. 8) ng/L, (246. 2 ±15.3)ng/L and(1.79 ±0.72)mg/L respectively which were significantly higher than postoperative and control group,the difference was statistically significant (P ＜ 0. 05 ) , while there was no significant difference between postoperative and normal control group ( P ＞ 0.05); The preoperative CD3+ , CD4+ and CD8+ T cells ratio of observation group were (0. 53 ± 0. 06) % , (0. 28 ±0.05)% and(0.21±0.04)% respectively which were significantly higher than postoperative and control group,the difference was statistically significant(P ＜ 0.05) , while there was no significant difference between postoperative and normal control group(P＞0.05). Conclusion Patients with gallstone had obvious abnormal serum IL-6, IL-8, TNF-α,CD3+ ,CD4+ and CD8+ T cells levels,surgery could not only eliminate inflammation,but also help the recovery of immune function in patients.

The effects of RHPS & APS on immune function in normal mice and immunosuppressed mice were studied. The results showed that RHPS and APS enhanced not only the phagocytosis of peritoneal mac-rophages in normal mice, but also antagonized completely the immun- Osuppresslve effect of prednisoione. RHPS and APS increased both the lymphocyte transformation rate induced by PHA and ANAE+ rate in normal mice, and antagonized also the suppressive effects of GY or PDS. No significant influence was found on the proliferation of rabbit lymphocyter treated with PHA in vitro. It seems that no obvious difference of effect was found on immune function between RHPS and APS.