Objective:To investigate the effect of Dan Sen on gastric mucosal Na +-K +-ATPase activity and gastric transmucosal potential difference in rats during severe intraperitoneal infection.Methods:The intraperitoneally infected rat model was established by cecal ligation and puncture(CLP).Assay of Na +-K +-ATPase activity in gastric mucosal tissue was conducted by biochemistry method.The electric-physiological recorder meter was used to measure gastric mucosal potential difference.Results:The activity of Na +-K +-ATPase markedly decreased in infected group at 3h after perforation,compared with the control group ( P

ObjectiveTo explore relation of muscle tension on recovery of upper limbs motor function of patients with hemiplegia after stroke.Methods102 hemiplegia patients were divided into 4 groups according to the degree of muscle tension,and treated with routine rehabilitation training. After one month,upper limbs motor ability and activities of daily living (ADL) of patients were assessed.ResultsAfter treatment,upper limbs motor ability and ADL of all patients were raised,but patients with light muscle tension had a better effect compared with those with serious muscle tension (P<0.01).ConclusionExcessive muscle tension will block the motor functional recovery of patients with hemiplegia after stroke.

Acute Lung Injury ; chemically induced ; pathology ; Animals ; Humans ; Lung ; pathology ; Nanospheres ; adverse effects ; Titanium ; adverse effects

Objective To investigate the imaging features in neonates with hypoglycemic brain injury by early and serial MRI.Methods Neonates who were admitted to neonatal department from May 2005 to Jul 2013 received MRI and diffusion-weighted imaging(DWI) scan within 7 days after hypoglycemia.Fortynine neonates were diagnosed with hypoglycemic brain injury.Thirty-four neonates received second MRI scan between 2 ～3 weeks after hypoglycemia.Seven neonates received third MRI scan.Results All the 49 neonates showed hyperintensity on DWI in the regions of occipital and parietal lobe for the first MRI scan(mainly involved 33 cases).Six cases combined frontal lobe and temporal lobe.Ten cases presented widespread cortex and white matter involvement.All the cases presented hypointensity on DWI for the affected area,T1 and T2 weighted image signal changes were not obvious.Some cases presented deep white matter and gray matter injury.Thirty-four cases received second MRI scan between 2 ～3 weeks after hypoglycemia,20 presented hypointensity on DWI,hypointensity on T1 weighted image and hyperintensity on T2 weighted image.Eleven cases with normal signals,and the other 3 were absorbing.Seven cases received third MRI scan,appeared encephalomalacia,myelin retardation,white matter volume decreased,hypoplasia of corpus callosum.The more severe the clinical symptoms was,the more severe the degree of brain injury showed.Conclusion Cerebral occipital and parietal regions are the most vulnerable in neonatal hypoglycemic brain injury.Early DWI for the imaging diagnosis of hypoglycemic brain injury should be taken within one week after hypoglycemia.Mild injury is recoverable,but severe would chang into necrosis and encephalomalacia.Some combined deep white and gray matter injury might related to hypoxia and ischemia.

Cultured porcine endothelial cells (EC) produce and secrete plasminogen activators (PA). If the serum free medium conditioned by vascular smooth muscle cells (SMC-CM) mixed with the medium conditioned by endothelial cells (EC-CM), the PA activities of the latter decreased significantly. Coeultivation of EC with SMC inhibited 70.7% PA activity of EC. Gel eletrophoretic analysis (SDS-PAGE) of SMC-CM followed by reverse fibrin autography demonstrated PA inhibitory activity in the molecular weight range of 49, 000-52, 000 similar to those identified by Laug (1985).In this study it was also investigated the effect of a Chinese herbal medicine-Radix Salviae Miltiorrhizae (RSM) on the inhibitory activity. SMC were incubated with RSM (0.1% in M-199, the final concentration was 1.5mg/ml) for 24hr. The SMC-CM collected before and after RSM treatment were mixed with EC-CM and urokinase respectively. The results show that the residual PA activities in EC-CM or urokinase samples mixed with SMC-CM treated with RSM were significantly higher than that mixed with SMC-CM without treatment with RSM. SMC-CM neutralized the PA activities in EC-CM or urokinase samples in a concentration dependent manner (P

0.05).One minute after intubation(T3),MAP and HR in the ETT group increased to(12.13?1.37) mm Hg and(96.3?4.7)/min respectively,which were significantly higher than those in the PLMA group [(9.98?1.21) mm Hg,t=6.443,P=0.000;and(88.3?5.6)/min,t=5.947,P=0.000].Three minutes after intubation(T4),the MAP and HR of the two groups were significantly different(P0.05).Five patients in the PLMA group had adverse reaction,that was significantly fewer than that in the ETT group(12 cases)(?2=4.022,P=0.045).Conclusions PLMA is applicable for posterior cervical spine surgery,since it is associated with less stimulation and adverse reaction,and stable hemodynamics.The success rate of intubation is high by using the procedure.

Objective To observe and discusses the application of larger-bore autologous arteriovenous fistula as dialysis pathways in diabetes patients with chronic renal failure. Methods 86 cases of diabetic patients with chronic renal failure were divided into elderly group and non-elderly group. The patients within each group were assigned randomly into Experimental subgroup and control subgroup. Patients underwent wrist autogenous arteriovenous fistula angioplasty.The surgical method was end to side anastomosis between cephalic vein and radial artery. Vertical incision on the radial artery is about 0.7 cm-0.8 cm in the control subgroup and vertical incision on the radial artery is about 1.0 cm-1.1cm in the experimental subgroup. Results The success rate of the operation in experimental subgroup(90.9%, 95.2%) was significantly higher than that of control group(77.3%, 85.7%). The differences were significant (P<0.05). There was no heart failure in the patients underwent surgery. Conclusions In diabetes patients with chronic renal failure need autogenous arteriovenous fistula angioplasty. Properly increasing the diameter of anastomosis can meet the demand on blood flow of hemodialysis without increase complication of heart failure.

OBJECTIVE： To investigate the possible role of the alteration of mitochondrial transmembrane potentials(Δ Ψ m) in arsenic trioxide(As2O3)-induced apoptosis of human gastric adenocarcinoma cells(SGC7901).METHODS： Cell apoptosis was assessed through morphology， cell viability， the percentage of sub-G1 cells and phosphatidylserine(PS) externalization.the Δ Ψ m was detected on flow cytometry through double staining of Rhodamine 123 (Rh123) and propidium iodide(PI).RESULTS： The As2O3-induced apoptosis was associated closely with the disruption of the Δ Ψ m.CONCLUSION： The disruption of the Δ Ψ m is one of the mechanisms of As2O3-induced apoptosis.

Esophageal carcinoma represents high incidence and mortality rate in China.Irinotecan (CPT-11) has shown promising activity in a number of gastrointestinal cancers,including esophageal cancer.This article reviews recent clinical trials of irinotecan-based chemotherapy and radiochemotherapy in esophageal carcinoma.

Objective: To investigate the effect of growth hormone(GH) and hypo calorie enteral nutrition(EN) on protein metabolism in patients after cardiac operations. Methods: 36 patients after cardiac operations were randomly divided into two groups. The test group received GH and EN, and the control group received EN only. The treatment was maintained 7 days postoperatively. Plasma albumin(A)?transferrin(TF)?prealbumin (PA)?retinol binding protein(RBP) on the preoperative day and day 1,4,8 postoperatively and nitrogen balance(NB) on the day 1,2,3,5,7 postoperatively were detected. Results: There was no significant difference of all plasma protein levels between two groups before operation and all decreased on the 1st postoperative day, but only RBP higher in the test group than that in the control. There was significant difference in TF,PA,RBP levels on the 4th and 8th postoperative day, the plasma A level on the 8th postoperative day, and NB on the 1st to 7th postoperative day between the two groups. Conclusion: Combined use of GH and EN can elevate plasma A,TF,PA,RBP levels and improve negative NB.