Objective The purpose of this study was to develop a new cultural method, so that the rat bone marrow stromal cells (MSCs) can well differentiate into osteoblasts in vitro and the cultured osteoblasts can be identified. Methods MSCs of rats were isolated and cultured, then the cultured cells were identified with inverted microscopy and undergone an HE stain for observing the morphology of living cells, and the cells were histochemically stained for examining the activity of cellular alkaline phosphatase (ALP), as well as were radio-immunologically analyzed for detecting the secretion of osteocalcin. Results The MSCs were typical fibroblast-like and possess a strang proliferative capability after cultured in vitro.The cellular ALP activity and osteocalcin secretion achieved the peak value from the fortieth day and maintained the status until the sixtieth day after cultured in vitro.Conclusion The data demonstrated that the new developed culture method has the advantages of short time, less risk of contamination and higher efficiency, and conducive to MSC's differentiating and proliferating into osteoblasts that do function well.

Objective] The two-step massage treatment of tennis elbow and simple massage therapy clinical curative effect around the elbow. [Method] A total of 80 cases of external humeral epicondylitis were randomly divided into the treatment group(42 cases) and control group(38 cases). The treatment group were treated with the combination of the neck and elbows. The control group was treated only with simple elbow massage. [Results] The treatment group cure rate was 71.4%, the total efficiency of 92.9%;control group, the cure rate was 34.2%, the total effective rate was 71%. The comparison between the 2 groups, the cure rate and total effective rate differences were significant( P＜0.01, P＜0.05). [Conclusion]Two-step massage has better cure effect on external humeral cpicondylitis.

Objective To observe the changes of serum interleukin and T lymphocyte subsets levels in patients with gallstones before and after treatment. Methods 72 patients with gallstones were treated as research subjects and 30 cases with normal physical examination as the control group,the serum interleukin and T lymphocyte subsets levels of observation group were detected before and after surgery. Results The preoperative serum IL-6, IL-8 and TNF-α levels of observation group were(61.5 ±4. 8) ng/L, (246. 2 ±15.3)ng/L and(1.79 ±0.72)mg/L respectively which were significantly higher than postoperative and control group,the difference was statistically significant (P ＜ 0. 05 ) , while there was no significant difference between postoperative and normal control group ( P ＞ 0.05); The preoperative CD3+ , CD4+ and CD8+ T cells ratio of observation group were (0. 53 ± 0. 06) % , (0. 28 ±0.05)% and(0.21±0.04)% respectively which were significantly higher than postoperative and control group,the difference was statistically significant(P ＜ 0.05) , while there was no significant difference between postoperative and normal control group(P＞0.05). Conclusion Patients with gallstone had obvious abnormal serum IL-6, IL-8, TNF-α,CD3+ ,CD4+ and CD8+ T cells levels,surgery could not only eliminate inflammation,but also help the recovery of immune function in patients.

Renal medullary cystic diseases are a large class of heterogeneous diseases,and they are common in clinic,most of them often progress to end-stage renal disease.With recent advances in genetics,increasing number of genes and genetic mutations has been identified with the etiology of renal medullary cystic diseases.Although genetic testing can provide mass data for diagnosis,clinical manifestation are even more important for clinical diagnosis,differentiation and genetic counseling for the patients.In this review,the classification,renal presentations,extra-renal presentations,and genetic analysis of renal medullary cystic diseases will be discussed.

Rhizome anemarrhena,sweet and bitter to taste,cold,in the lung,stomach and kidney meridian,has the function clearing away heat and purging pathogenic fire,nourishing Yin and moistening dryness.Zhang Zhong Jin in "Essential Prescriptions of the Golden Chamber "with a different combination of herbs and Rhizome anemarrhenae,reflects the multiple efficacies of TCM and compatibility.

The tri-plate horizontal mould with nonrigid surgical sponge inserts was developed to establish an industrial system for shaping surgical sponge which is used for cataract operation. The conception on an insertion mechanism of nonrigid flat inserts was firstly suggested by us. The prototype horizontal moulds with tri plate have been designed and manufactured. The experiment results of hundred thousand of times injection shaping have proved that the conception of the design is rational. The structure of shafting mould for nonrigid flats has been confirmed. Present article discussed the design and working mechanism of the insertion in the horizontal injection mould.

Cyclic alternating pattern (CAP) during sleep is a kind of cyclic oscillation of EEG pattern in non-rapid eye movement sleep(NREM sleep), which reflects microstructure of sleep. It has been identified that CAP plays an important role in keeping the integrity of sleep. The purpose of the interview is to introduce the recording, identification, physiological meaning and clinic implication of CAP.

Recently, heme oxygenase-1 (HO-1) has been emphasized in the area of intensive care unit (ICU) and other domains. HO-1 can catalyze the decomposition of heine and the products of its enzymatic activity, including carbon monoxide, biliverdin, and bilirubin and ferritin can play a significant cytoprotec-tive role in antagonizing inflammation, protecting cells from oxidative injury and cellular stresses. In the re-search of severe acute pancreatitis (SAP), researchers have realized that it is of great importance to antago-nize the overflow of cytokines in order to improve the therapeutic effect of SAP. In this article, we give a re-view of the research progress of HO-1 and its relationship with SAP.

Objective To investigate the effect of intravenous injection of tranexamic acid on reducing bleeding effect in invasive total hip replacement.Methods In our hospital from April 2015 to April 2016, 50 cases of total hip joint replacement patients as the object of study, patients were randomly divided into observation group and control group, 25 cases in each group.The control group were injected with 0.9% NaCl injection;observation group received intra-articular injection of tranexamic acid after suture, the transfusion volume of blood, complications, hemoglobin concentration, and rate of blood transfusion were compared between the two groups.Results The rate of blood transfusion in the control group was 52.0%, significantly higher than that of the observation group (24.0%), and the difference was statistically significant (χ2 =4.16, P<0.05).The drainage (260.1 ±78.6) mL and transfusion volume (230.8 ±60.1) mL in observation group were better than control group (370.04 ±56.2)mL and (380.2 ±40.6)mL,with statistical significance (P <0.05).The hemoglobin concentration in observation group was lower than control group(P <0.05).There were 2 cases of hematoma, 5 cases of paining in observation group and 3 cases of hematoma, 6 cases of paining in control group, with no statistical significance (χ2 =0.368, P>0.05).Conclusion Tranexamic acid is the main method for prevention of postoperative bleeding, but could not reduce postoperative complications.

Objective To study MRI characteristics and value in the diagnosis of nasopharyngeal carcinoma with skull base erosion. Methods 86 cases of nasopharygeal carcinoma with positive nasopharyngeal pathology and symptoms of skull base erosion were selected. Axial T1WI, T2WI and coronal, sagital T1WI were performed. 47 cases had axial, coronal and sagital T1WI examination post Gd-DTPA injection. Results Areas of skull base involvement were as follow: 56 cases with pterygoid process, 54 with clivus, 28 with foramen ovale (including sphenoid wing), 27 with petrous apex, 1 with occiptal bone. 71 cases had more than one area involvement. There are four patterns in our group: ①Defect in the signal avoid cortex of skull base; ②High signal intensity of marrow was replaced by tumor; ③Contrast enhancement in abnormal bone; ④String like enhancement passing nerve foramen in skull base. 44 cases included intracranial abnormal. Conclusion MRI is the preferred technique in dementstrating skull base involvemetn of nasopharyngeal carcinoman.