AIM: To establish the quality standard of Yupingfeng Oral Liquid(Radix Astragali,Radix Sapo-shnikoviae,Rhizoma Atractylodis Macrocephalae,etc.); METHODS:Rhizoma atractylodis macrocephalae was (identified) by TLC,Radix Saposhnikoviae was identified by HPLC;astrayloside Ⅳ was determined by HPLC.( RESULTS):The recovery of astragloside Ⅳ was 96.7%,RSD was 1.74%.The TLC sports developed were fairly clear,and the blank test showed no interference. CONCLUSION:The mothod developed is simple and accurate with good reproducibility,and the mothod can be used for quality control of Yupingfeng Oral Liquid. withgood

Cryoglobulinemia ; physiopathology ; Humans ; Male ; Middle Aged ; Peripheral Nervous System Diseases ; diagnosis ; etiology ; Serologic Tests ; Syphilis ; diagnosis

Objective:To develop a method for determining deoxynivalenol in four kinds of traditional Chinese herbal medicines. Methods:After being extracted by water, purified by an immunoaffinity column, deoxynivalenol was analyzed by LC-MS-MS. Results:The calibration curve was linear within the range of 2-50 ng·ml-1 for deoxynivalenol. The recovery was 68. 7%-88. 3%. Conclusion:The method is simple, sensitive and accurate in the determination of deoxynivalenol in Semen Ziziphi Spinosae, Semen Sojae Praepara-tum, Semen Coicis and Fructus Psoraleae.

This paper aims to establish a method for the determination of sulfur dioxide in sulfur fumigation Chinese herbs. Sample powder and hydrochloric acid solution were isolated by paraffin layer in order to avoid early reactions, with the generation of sulfur dioxide, headspace with airtight needle was used to transfer sulfur dioxide into gas chromatograph, and detected with thermal conductivity detector. The analytical performance was demonstrated by the analysis of 12 herbs, spiked at four concentration levels. In general, the recoveries ranging from 70% to 110%, with relative standard deviations (RSDs) within 15%, were obtained. The limit of detection (LOD) was below 10 mg x kg(-1). Standard addition can be used for low recovery samples. The method is simple, less time-consuming, specific and sensitive. Methods comparison revealed that gas chromatography is better than traditional titration in terms of method operability, accuracy and specificity, showing good application value.

Objective:To improve the quality standard for Anweiyang capsules. Methods: TLC was used to identify Glycyrrhiza inflata Bat. Licochalcone A was determined by RP-HPLC. Using a C18 Column, the mobile phase consisted of acetonitrile-0. 1% phos-phoric acid(40:60), and the detection wavelength was at 376 nm. Results:The herb could be identified by TLC. For licochalcone A, the linear range was from 25. 563 to 1 533. 798 ng, and the average recovery was 99. 8%(n=9). Conclusion:The method is simple and accurate, which can be used to improve and control the quality of Anweiyang capsules.

Objective To evaluate the effects of laparoscopic choledochal cyst excision for treatment of congenital choledochal cyst.Methods Forty patients with congenital choledochal cyst were randomly divided into control group and observation group: the control group underwent open abdominal excision of choledochal cyst,while the observation group had laparoscopic resection of choledochal cyst.Results The operations of all the 40 cases in the 2 groups were successfully completed.The operative time was longer in the observation group(P

In this paper, a systematical and retrospective review was made on how the drug purchasing system of Chinese healthcare institutions has evolved from decentralized purchasing to centralized purchasing based on four stages as budding stage, initial stage, adjustment stage and deepening stage. In each stage, the working characteris-tics, achievements and problems have been analyzed. Targeting at the problems and challenges confronted with cur-rent drug purchasing, recommendations was made to reform and improve public hospitals’ centralized purchasing sys-tem, such as persisting centralized purchasing online at provincial level, sticking to “four favorable” principles, breaking through the mechanism of compensating medical cost with drug-selling profits, promoting coordinated inter-action reforms in healthcare insurances, distribution and pricing, strengthening comprehensive services and supervi-sion, etc.

To investigate the effects of icariin, an effective extract from traditional Chinese medicine Epimedium pubescens with the function of tonifying kidney, in promoting osteogenesis of mesenchymal stem cell line C3H10T1/2, and to explore the underlying mechanism.

ObjectiveThe apoptosis and anti-proliferation effects of photodynamic methods on cervical cancer and ovarian cancer cell lines were studied to explore the advantage of photodynamic therapy in gynecological cancer.MethodsCervical cancer cell line (Hela) and ovarian cancer cell line (SK()V3) were treated with photosensitizer (5-ALA) at different doses and then radiated by laser with energy scopes. The morphological characteristics were observed by light microscopy and electronic microscopy. Inhibition effects and cell apoptosis were studied by MTT assay and flow cytometry (FCM) ResultsThe cytomorphosis and shrinkage, nucleus condensation and anachromasia, dense chromatin lining along cell membrane were observed in both cell line receiving photodynamic therapy.Through transmission electron microscope (TEM), we found nucleus chromatin gathering along membrane of cell nuclear and apoptotic body formed. The cell inhibition rates were 12.9％-90.7％ and 3.9 ％-70.0％, respectively in Skov3 cell and HeLa cell treated with 5-ALA of different concentrations (0.1, 0.5, 1.0, 2.5 mol/L) and receiving laser of different doses (0.1,0.5, 2.5, 12.5 J/cm2). The concentration of the photosensitizer and the dose of laser influenced the optical density(A) of cell lines (P＜0.01). The photo sensitizer was related strongly to laser (P＜0.01). The optical density of the two cell lines decreased with the increases of photo sensitizer concentration and laser dose ( P＜0.01 ),showing concentration- and time-dependent manner.Conclusions Photodynamic therapy inhibits the growth of ovarian cancer and cervical cancer cell line.

AIM: To establish the GC method for the determination of linalool of Rhizoma Homalomenae. METHODS:The column consisted of Fused Silica Capillary Polyethylene Glyol(30 m?0.25 mm?0.25 ?m),column temperature was at 120 ℃,Injection port temperature at 260 ℃,Detector temperature at 300 ℃,flow rate was 1.0 ml/min. RESULTS: Linalool has a good linearity in the range of 0.011 985～1.997 5 ?g.The recovery rate was 99.2%,RSD was 1.63%. CONCLUSION: The method developed is simple and accurate with good reproducibility,and can be used for quality control of Rhizoma Homalomenae.