Objective To observe clinical effect of treating acute cerebral infarction with Danhong injection combined with Edaravone injection. Methods 58 cases with acute cerebral infarction were randomly recruited into a control group and a treatment group. The control group was treated with edaravone injection, 30 mg, once daily, and for continuous 14 days. The treatment group was treated with dan hong injection, 20 ml, once daily, and for continuous 14 days based on the control group. The clinical effect and adverse reaction were observed in both groups after the treatment. Results The total effective rate was 86.2% and 62.1% in the treatment group and the control group respectively. The clinical effect in the treatment group was significantly higher than that of the control group (χ2=3.561, P＜0.05). Conclusion Danhong injection combined with edaravone injection was more effective in treating acute cerebral infarctionthan edaravone injection exclusively.

Liver transplantation rapidly has become the best choice for pediatric patients with end-stage and metabolic liver disease, since the first liver transplantation succeeded in 1989. In pediatric liver transplantation, significantly improved short-term and long-term survival is attributed to advances in surgical techniques and reasonable immunosuppression regimens in the last 20 years. However, postoperative infections, which remain the most common complication following pediatric liver transplantation, severely affect posttransplantation morbidity and mortality. This paper summarizes the infections after liver transplantation in children and rational drug use.

Objective To study the expression of connexin 43 in human cardiac conduction system(CCS) and heart. Methods The distribution of CX43 was studied immunohistochemically using of SABC method with antibody CX43. Results Smaller amounts of CX43 can be seen in the nodal myocytes at the periphery of sinoaterial and atrioventricular node whereas in the myocytes at the center of nodes were negative. In the Purkinje fibers, abundant expression of CX43 was found, and in atrial and ventricular myocardium contained abundant amounts of CX43, especially in the intercatalated disk.Conclusion The expression of connexin 43 varies greatly in specific regions of the human heart with different conduction properties. These differences may play a role in regulating electric impulse of cardiac conduction.

Objective:To evaluate the understanding and success rate in diagnosis and treatment of adrenal cortical tumor in children.Methods:6 cases were analyzed retrospectively about diagnosis,treatment,pathomorphological changes and prognosis.Results:6 cases of adrenal cortical tumor were treated surgically,which include 4 of cases Cushings syndrome,4 cases developed HBP,in 1 case developed hyperglycemia and in another case had hypokalemia developed.1 patient died of Ⅲ AVB(atrial ventricle block,AVB) during operation.Conclusion:Early diagnosis and early surgical interventions are crucial for treatment.Perioperative management plays an important role in improving prognosis.

The present study describes the effects of carbamate anti-cholinesterase agents and other drugs on the binding sites and on the turnover of nicotinic acetylcholine receptors (N-AChR). The direct binding studies with 125I-?-Bungarotoxin have shown that the neostigmine, prostigmine, pyridostigmine, and ambenonium specifically blockada the N-AChR binding sites, but physostigmine has no directly effect on the N-AChR binding sites.Small dose of ambenomine increases degradation rate of surface N-AChR and it decreases the rate of new N-AChR incorporation into membrane. The bindings of sodium phenobabital to N-AChR sites are very similar to those of d-tubocurarine. The parathion(E605)has no direct bindes to N-AChR sites. The density of extrajunc-tional N-AChR sites is increased and the RBI is 1.46.

The dynamic changes of the transcriptional activities of the nuclei of the rat liver and skeletal muscle cells were observed in the 6th, 12th, 24th, 48th, and 72nd hours after the animals were inflicted with 30% TBSA third degree burns.It was found that the incorporation of [3H]-UTP into the liver cell nuclei in vitro increased significantly in the 6th and 12th postburn hours. The peak of elevation occurred in the 6th hour postburn (from 7408 ?690 cpm/50?g DNA of normal to 10175 ? 1227 cpm/50?g DNA, P

The present study reports direct effects of allosteric snake neurotoxin (MN-81) on the nicotinic acetylcholine receptor (N-AChR) and on the transmission of nuro-muscular junction. when one dose of MN-81 with 50 ?g/kg body weight was injected into the mice, the number of N-AChR binding sites was not changed. when one dose of MN-81 with 250 ng/kg body weight was injected, 23 per cent of total N-AChR were occupied by MN-81 molecules. But when repeated injection of MN-81 was carried out N-AChR binding sites were increased by about 34 percent. Assay of phrenic nerve-diaphragm showed that the low close of MN-81 increased contractile amplitude of isolated rat diaphragm and rabbit diaphragm in situ, while an increase in the concentration of MN-81 (10-4g/ml) led to blockade of nuromus-cular transmission.

Regulatory T cells (Tregs) play important roles in immune homeostasis and tolerance.Recently, several characteristic studies on tissue-resident Tregs have been drawing wide attention.Some tissue-resident Tregs express some special molecules and are involved in the regulation of local tissue functions apart from immune regulation.This article focuses on the functions of some tissue-resident Tregs, such as fat-resident Tregs participating in the regulation of insulin resistance, muscle-resident Tregs in the regeneration and repair of damaged skeletal muscle, and thyroid-resident Tregs in the regulation of thyroid function, aiming to help medical graduates further understand the phenotypes and functions of tissue-resident Tregs.

Objective:To carry on all-directions and multi-layer search and analysis about the hospital information, and to provide the informatin search, data analysis and the decision supports for each kind of personnel in hospital. Methods:The data within the hospital information system was carried on withdraw, setting up the model with the data warehouse technique. Results:The frame of the hospital data warehouse was built up and multidimension systems were in application. Conclusion:The data warehouse is a kind of developing technique of the hospital information system, will provide the most useful information to the decision support for the hospital.

Objective: To clone the Chinese MxA gene and to construct the recombinant adenovirus plasmid carring human MxA gene for further study.Methods: The peripheral blood mononuclear cells(PBMCs) were isolated by gradient fractionation,then total RNA was extracted from the PBMCs.The MxA gene was amplified by RT-PCR using the primers based on the published sequence of MxA.The PCR product,double-digested with restrictive endonucleases NotI and XbaI,was cloned into pAdTrack-CMV by molecular cloning technique.The plasimid pAdTrack-MxA was linearized with Pme I,and subsequently co-transformed into electrocompetent BJ5183 cells with an adenoviral backbone plasmid(pAdEasy-1).Recombinants were selected for kanamycin resistence and confirmed by PacI.Finally,the linearized recombinant plasmid was transfected into HEK 293 cells.Recombinant adenoviruses were generated within 7 to 12 days,then viral tite was checked by GFP.Results:Sequencing analysis revealed that the amino acid sequence of MxA gene had only one amino acid residue displace from isoleucine to valine(compared with that of published sequence) although there were five variations in nucleotide sequence.The restrictive endonuclease analysis confirmed that correct recombinant adenovirus plasmid was constructed.24 hours after transfection,the fluorescence was observed in 293 cells,and viral title checked by GFP was 1.59?108(pfu/mL).Conclusion: The MxA gene is cloned from a Chinese donor and a recombinant adenovirus vector containing human MxA gene is constructed successfully.It lays the foundation for further study of gene therapy for HBV.