Child ; Child Welfare ; trends ; China ; Humans

Human LAK cells induced from peripheral blood mononuclear cells with recombinant interleukin2 (rIL-2) incubation, can be cloned in semisolid medium and proliferated well in liquid cultures containing rIL-2. Phynotypie analysis by flow cytometry showed that these cloned LAK cells were T_3(+), T_4(-), T_8(+), Tac(+), DR(+) and NKH1(-)/(+). Cultured with 1?10~5/ml normal bone marrow mononuelear cells (BMMNC)for 0.5-hr prior to semisolid culture system for CFU-GM, the cloned LAK ceils at 2?10~5/ml showed inhibitory effect on CFU-GM that the colonies were decreased by 43.8% of control though 0.5 or 1?10~5/ml LAK cells did not. Morever, a dose-dependent inhibition of CFU-GM was noted when LAK cells pre-inbated with BMMNC for 4-hr in doses of 0.5 -4?10~5/ml that the inhibitory rates of CFU-GM were 58.8, 67.5, 88.8 and 96.3% at0.5, 1, 2, 4?10~5/ml LAK cells addition respectively. Using double-layer agar cultures, inhibitory activity for CFU-GM was also observed only when LAK cells at 4?10~5/ml without contacted with BMMNC. No significant difference of inhibitory effects on CFUGM growth was found between autologous and allogenie clotted LAK cells under same condition. These data suggeste that LAK cells inhibited growth of myeloid progenitor cells without MHC-restriction and the mechanism might involve cell-to-cell interaction and/or some soluble factors secreted by LAK cells.

Objective:To develop an information management system for equipment archives management, maintenance, measuring and statistics for hospital medical equipment.Methods:VS 2008 and SQL Server 2008 were used as the development tools. B/S software architecture was applied to the design of system function modules.Results: The system structure of B/S model could satisfy the requirements form the equipment archives management, maintenance, measuring and statistics of hospital medical equipment. The desired functional modules were implemented, which adapted to the acquisition and utilization of hospital medical equipment management.Conclusion: The information management system for hospital medical equipment can acquire and maintain medical equipment information, with digitalizing information of equipment archives, measuring and statistics, and networked the maintenance flow of medical equipment. The information sharing has been realized and the management level of hospital medical equipment has been improved.

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Humans ; Hydrofluoric Acid ; Occupational Exposure ; prevention & control ; Protective Devices

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Objective To investigate the effects of intrathecal different doses of dexmedetomidine hydrochloride in spinal block by ropivacaine hydrochloride . Methods Forty lower limb surgery scheduled for elective under spinal anesthesia, were randomly divided into 2 groups (n = 20 each): the control and the treatment groups.The control and the treatment group were intrathecally injected with 4,12 μg dexmedetomidine hydrochloride respectively.The 0.75% ropivacaine hydrochloride 1.5 mL was injected for spinal anesthesia.SBP,DBP,HR,SpO2 and Ramsay Sedation Score were recorded before the spinal anesthesia conduct and thereafter every five minutes. And the onset and duration of block were recorded,adverse reaction like nausea, vomiting and respiratory depression were also observed. Results Compared with the control group,the onset of sensory block was shorter [(6.9±2.6) min vs (8.7±2.9) min] (P<0.05),and the duration of sensory and motor block was longer in the treatment group[(130.8±30.1) min vs (115.9±23.9) min] (P<0.05) and [(145.9±29.0) min vs (130.0±30.1) min] (P<0.05). Conclusion Intrathecal dexmedetomidine hydrochloride at 12 μg improves anesthesia via shortening the sensory block onset and prolonging sensory and motor block,which maintains hemodynamically stable,and does not generate adverse reactions as nausea,vomiting,bradycardia and respiratory depression.

Objective To evaluate the short-term efficacy and safety of combination therapy with amlodipine and terazosin in middle aged and old male patients with essential hypertension.Methods Randomized,prospective,parallel study was carried out in middle aged and old male patients with essential hypertension in Anqing community between August 2005 and February 2006.Antihypertensive efficacy and safety of the combination therapy were evaluated in 508 patient who completed the study.Results After 4 weeks treatment,the average reduction of SBP were 4.0?15.0,17.5?15.8 and 20.0?15.9 mm Hg in Terazosin group,amlodipine group and combination group,respectively(P

Objective To explore the clinical effect of laparoscopic myomectomy.Methods According to the digital table method,75 myoma patients were divided into the study group (42 cases received laparoscopic myomectomy) and the control group(33 cases received transabdonmial myomectomy).The operation time,the time of intestinal function recovery,the amount of bleeding and the postoperative hospital stay time of the two groups were recorded and compared.Results The amount of bleeding,the time of intestinal function recovery and the postoperative hospital stay time of the study group were (111.50 ± 19.38) ml,(17.76 ± 3.64),(4.95 ± 0.54) d,respectively,which were significantly less than those of the control group [(131.15 ± 27.85) ml,(31.64 ± 5.70) h,(7.12± 1.08)d] (t =3.60,12.81,11.35,all P ＜ 0.01).Conclusion Laparoscopic myomectomy has the advantages of less trauma,quicker recovery,shorter hospitalization time etc,so it is worthy of promotion and application in clinic.