Objectives:To investigate the effects of nutritional support by the way of peripheral vein(PV) in the patients of severe acute pancreatitis(SAP). Methods:The treatment results of nutritional support by the way of PV was observed retrospectively within the latest eight years in the patients of SAP. Results:31 cases were cared,and no complications relating to the use of PV in TPN happened. Conclusions:Nutritional support is an important and effective treatment in SAP patients,and the use of PV is an effective and safety route.

OBJECTIVETo investigate the biological characteristic of ectomesenchymal stem cells (EMSC) and the differentiation of p75(+)EMSC and influencing factors.

METHODSThe immunohistochemical staining method was used to observe the migration of EMSC from 12.5 d SD rat embryonic facial process. Then EMSC was labeled by p75 neurotrophin receptor, and the cell cycle and stem cell surface antigens of the p75(+) EMSC was examined.

RESULTSImmunohistochemical staining showed that stem cells from the cranial neural crest migrated to embryonic rat facial process at 12.5 days. The sorting rate of the p75(+)EMSC was 6.1%. The proportion of the p75(+)EMSCs' S/G2/M phase was stable during subculture. The special substances CD29, CD146 and Stro-1 were marked for p75(+)EMSC, and the expressions of the markers were all higher (> 90%) in p75(+) EMSC.

CONCLUSIONSThe embryonic tooth did not start to grow on the conception 12.5 days of SD rats. The p75(+) EMSC after sorting had stable proliferation ability and had stem cell characteristics during subculture and didn't start differentiation.

Animals ; Biomarkers ; metabolism ; Cell Cycle ; Cell Differentiation ; Cell Division ; Cell Movement ; In Vitro Techniques ; Mesenchymal Stromal Cells ; chemistry ; physiology ; Rats ; Rats, Sprague-Dawley ; Receptor, Nerve Growth Factor