1.Diagnostic Value of mDIXON Quant MRI for Vertebral Metastasis
Jia ZHANG ; Haibo TAO ; Yingying DING ; Mei LI ; Shiyun SUN ; Manzhu LI ; Kun LI
Cancer Research on Prevention and Treatment 2021;48(9):888-892
Objective To evaluate the diagnostic efficacy of mDIXON Quant for vertebral metastasis. Methods We retrospectively analyzed MRI images of 152 patients with clinically diagnosed malignant tumors and vertebral metastases. Scanning sequence included T1WI, STIR, mDIXON Quant and enhanced T1WI. We compared the diagnostic efficiency between T1WI and FF images for vertebral metastases. FF values between normal vertebral body and vertebral metastases were quantitatively compared, and the diagnostic efficacy of FF value was evaluated by ROC curve. Results The sensitivity, false positive rate, false negative rate, negative predictive value and accuracy of FF images in the diagnosis of vertebral metastases were all higher than those of T1WI images. The FF value of vertebral metastasis was significantly lower than that of normal vertebral body (
2.The capacity and biological characteristic of the p75 neurotrophin receptor-positive ectomesenchymal stem cell in vitro.
Manzhu ZHAO ; Xiujie WEN ; Gang LI ; Kun YANG ; Lin LI ; Ming TANG
Chinese Journal of Stomatology 2015;50(2):103-109
OBJECTIVETo investigate the biological characteristic of ectomesenchymal stem cells (EMSC) and the differentiation of p75(+)EMSC and influencing factors.
METHODSThe immunohistochemical staining method was used to observe the migration of EMSC from 12.5 d SD rat embryonic facial process. Then EMSC was labeled by p75 neurotrophin receptor, and the cell cycle and stem cell surface antigens of the p75(+) EMSC was examined.
RESULTSImmunohistochemical staining showed that stem cells from the cranial neural crest migrated to embryonic rat facial process at 12.5 days. The sorting rate of the p75(+)EMSC was 6.1%. The proportion of the p75(+)EMSCs' S/G2/M phase was stable during subculture. The special substances CD29, CD146 and Stro-1 were marked for p75(+)EMSC, and the expressions of the markers were all higher (> 90%) in p75(+) EMSC.
CONCLUSIONSThe embryonic tooth did not start to grow on the conception 12.5 days of SD rats. The p75(+) EMSC after sorting had stable proliferation ability and had stem cell characteristics during subculture and didn't start differentiation.
Animals ; Biomarkers ; metabolism ; Cell Cycle ; Cell Differentiation ; Cell Division ; Cell Movement ; In Vitro Techniques ; Mesenchymal Stromal Cells ; chemistry ; physiology ; Rats ; Rats, Sprague-Dawley ; Receptor, Nerve Growth Factor