Adult ; Cysts ; surgery ; Female ; Humans ; Male ; Middle Aged ; Nasal Cavity ; Nasal Surgical Procedures ; methods ; Nose Diseases ; surgery ; Treatment Outcome ; Young Adult

Aim To observe the effect of dopamine (DA) and its antagonist on the electrical activities of pain excitation neurons (PEN) in the caudate nucleus (Cd) of morphine dependent rats. Methods The right sciatic nerve was stimulated by train of electrical impulses as noxious stimuli to morphine dependent rats.The electrical discharges of PEN in Cd were recorded with glass microelectrode to observe influences of intracerebroventricular (icv) injection of DA and droperidol on the electrical activities of PEN. Results DA could decrease excitabilities of PEN in Cd of morphine dependent rats. Conclusion Results suggest that the depression of DA on PEN response in Cd of morphine dependent rats might be blocked by droperidol.

OBJECTIVE: To investigate the expression of CDK4 and p16 in laryngeal squamous cell carcinoma (LSCC) tissues. METHOD: The expressions of CDK4 and p16 in 30 cases of LSCC tissues and 20 cases of edge tissues were detected by immunohistochemical technology SP method, and discuss their correlation with clincial pathology and clinical stage of LSCC. RESULT: The positive rates of CDK4 and p16 were 63. 3 %,46. 7% in LSCC tissues, and the positive rates were 25%, 90% in edge tissues. The expression of CDK4 in LSCC tissues was significantly higher than that in edge tissues(P<0. 05), which was not associated with the clincial pathology and clinical stage(P> 0. 05); The expression of p16 in LSCC tissues was significantly lower than that in edge tissues(P<0. 05), it was associated with the clincial pathology (P<0. 05), but not associated with clinical stage(P>0. 05) ;there is a negative correlation between CDK4 and p16 (r= -0. 786, P<0. 05). CONCLUSION Low expression of p16 and high expression of CDK4 may play an important role in the development of LSCC and the low expression of p16 in LSCC tissue could be used as important reference markers of malignant degree of tumour.
Biomarkers, Tumor ; Carcinoma, Squamous Cell ; metabolism ; Cyclin-Dependent Kinase 4 ; biosynthesis ; Cyclin-Dependent Kinase Inhibitor p16 ; biosynthesis ; Head and Neck Neoplasms ; metabolism ; Laryngeal Neoplasms ; metabolism ; Prognosis ; Squamous Cell Carcinoma of Head and Neck

OBJECTIVE: To explore the prophylactic effect of Budesonide on the expression of IL-4,IL-5 in nasal mucosa in model of minimal persistent inflammation of allergic rhinitis in rats. METHOD: Eighty SD rats were randomly divided into allergic rhinitis group (A group), experimental (B group), control group (C group) and negative control group (D group). A group was made for model of allergic rhinitis. B and C group were made for model of the lightest persistent inflammatory response. After the models were established, half of rats in the A group, B group, C group and D group were executed, and EOS infiltration and the expression of IL-4, IL-5, ICAM-1 were observed in nasal mucosa. The remaining rats of B group were given budesonide (64 microg/side/time, twice/day) treatment for 2 weeks. A, C, D group were given nasal spray with normal saline for 2 weeks. After that A, B, and C groups were stimulated with 1% OVA daily for one week, D group were given nasal spray with normal saline. All rats were executed after excitation, EOS infiltration and IL-4, IL-5 expression were observed. RESULT: After the drug treatment, B group only had a small amount of mucous EOS infiltration and had no significant difference with D group, but in A and C group EOS had heavy infiltration. Gray value of the IL-4 positive areas in B group were significantly different compared with A and C group (P < 0.05), A group and C group had no significant difference (P > 0.05). Distribution of IL-5 positive signals was similar with that of IL-4. CONCLUSION Budesonide MPI application could significantly inhibit the allergic.
Animals ; Budesonide ; therapeutic use ; Disease Models, Animal ; Female ; Interleukin-4 ; metabolism ; Interleukin-5 ; metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; Rhinitis, Allergic ; metabolism ; prevention & control

Aim To investigate the effects of cholecys-tokinin octapeptide(CCK-8)on the withdrawal symp-toms of morphinistic rats by the observation of praxiology,and change of expression of proto-oncogene c-jun in caudate nucleus(Cd) after CCK-8 injected by immunohistochemistry.Methods Animal praxiology evaluation and immunohistochemistry were used.The influence of CCK-8(10 mg?L-1,1 ?l) on the withdrawal symptoms and the expression of c-jun of morphinistic rats was observed.Results Morphinistic rats had obvious praxiological changes in the withdrawal symptoms compared with normal saline rats;the scores of withdrawal symptoms decreased most obviously at withdrawal 40 h after Cd injection of CCK-8;the expression of c-jun protein in Cd decreased in morphinistic rats,but increased after Cd injection of CCK-8.Conclusions Models of morphinomania rats were successfully built.The Cd injection of CCK-8 could decrease the withdrawal symptoms of morphinomania rats.The CCK-8 could increase the expression of c-jun protein in Cd.These findings suggested that CCK-8 could regulate the production of withdrawal symptoms and the expression of c-jun protein in Cd of morphinomania rats.

Objective:Deeper studies have been rarely reported on the characteristics and influencing factors of the psychological health of army nurses and on the measures for raising their ability of military stress management. To gain an insight into the psychological state of army nurses in the present military streamlining and to explore its countermeasures. Methods:We investigated the psychological state of 123 army nurses from 6 military hospitals by psychometrical methods and psychological interviews,and analyzed the results. Results:Of the total number of subjects,70.73% scored

OBJECTIVE: To preliminarily determine the gene mutation frequency and the hotspots in Henan province, we analysed the deafness-related gene mutation in patients with non-syndromic hearing loss (NSHL). METHOD: Genomic DNA samples of 100 patients with NSHL in Henan province were extracted from peripheral blood after clinical history inquiry and clinical examination, Four common deafness genes GJB2, SLC26A4, mitochondrial 12SrRNA, and GJB3 were detected by Sanger sequencing method,and then data analysis were conducted. RESULT: Among 100 patients with NSHL. the gene mutation frequency was 44%. In these patients, 29 cases had GJB2 mutations, 13 cases had SLC26A4 gene mutations, and 3 cases had mitochondrial 12SrRNA mutations. CONCLUSION Among the patients with NSHL in Henan province, the most frequent mutation causing hereditary deafness was mutation in GJB2, followed by SLC26A4,and it will provide a theoretical basis to determine the etiology of deafness in Henan Province.
China ; Connexin 26 ; Connexins ; genetics ; DNA Mutational Analysis ; DNA, Mitochondrial ; genetics ; Deafness ; genetics ; Gene Frequency ; Humans ; Membrane Transport Proteins ; genetics ; Mutation ; RNA, Ribosomal ; genetics ; Sulfate Transporters

OBJECTIVE: To compare the efficacy of Epley's manoeuvre and Epley's manoeuvre plus labyrinthine sedative in the treatment of benign paroxysmal positional vertigo. METHOD: Eighty-four patients with benign paroxysmal positional vertigo were included in a randomized study. Forty-two cases in control group were treated with Epley's manoeuvre alone and the other 42 cases in treated group received Epley's manoeuvre together with Betahistine mesilate tablets, flunarizine hydrochloride, and extract of Ginkgo biloba leaves tablets. Both groups were analyzed after one week and four weeks, and recurrence was observed after half a year. RESULT: After one week treatment, cure rate and total effective rate of treated group was higher than that of control group (78.57% vs. 50.00% and 92.86% vs. 80.95%, P < 0.05, respectively). After four weeks treatment, there was no significant difference in cure rate and total effective rate between two groups (80.95% vs. 71.43% and 95.24% vs. 90.48%, P > 0.05, respectively). After half year, recurrence was found in 3 patients in treated group compared with 7 patients in control group (7.14% vs. 16.67%), and the difference was statistically significant (P < 0.05). CONCLUSION Epley's manoeuvre is the best treatment method of benign paroxysmal positional vertigo together with the medicine. It is easy, safe and effective, and the healing time can be shortened.
Adult ; Aged ; Benign Paroxysmal Positional Vertigo ; Female ; Humans ; Hypnotics and Sedatives ; therapeutic use ; Male ; Middle Aged ; Musculoskeletal Manipulations ; Treatment Outcome ; Vertigo ; drug therapy ; therapy

OBJECTIVE: To investigate the relation between IL-4/STAT6 and allergic rhinitis by comparing expressions of IL-4 and STAT6 in normal nasal mucosa and allergic rhinitis models, to explore the influence of glucocorticoid on IL-4, and STAT6 expression, and then to elucidate further the pathogenesis of AR and the mechanism of glucocorticoid. METHOD: Forty-five guinea pigs were divided into three groups: normal control (NC) group, allergic rhinitis group (AR) and glucocorticoid (Glu) group (15 each). Animals in AR and Glu groups were sensitized with egg albumin, and in NC group were treated with normal saline as control. After sensitization and reproduction of AR model, rats in AR group received no treatment, while those in Glu group were treated with glucocorticoid (50 microl/one side/time, once a day) for 5 days. The changes in behavior was examined, and pathology of nasal mucosa were observed with HE staining, and the protein expressions of IL-4 and STAT6 in the nasal mucosa were detected by immunohistochemical technique. RESULT: Compared with NC group, the frequency of sneezing and nose-scratching, and the expressions of IL-4 and STAT6 were increased obviously, but the opposite findings were observed in Glu group. CONCLUSION IL-4 and STAT6 are related to the pathogenesis of allergic rhinitis and may be the main factors for eosinophil infiltration in allergic rhinitis. Glucocorticoid may produce a therapeutic effect by intervening the expression of IL-4 and STAT6.
Animals ; Disease Models, Animal ; Glucocorticoids ; pharmacology ; Guinea Pigs ; Interleukin-4 ; metabolism ; Male ; Nasal Mucosa ; drug effects ; metabolism ; pathology ; Rhinitis, Allergic, Perennial ; metabolism ; pathology ; STAT6 Transcription Factor ; metabolism

Objective To explore the clinical effects of sequential flap of thigh perforator for closure of the anterolateral thigh free flap donor site.Methods From March,2014 to May,2015,8 consecutive patients underwent elective anterolateral thigh free flap reconstruction for limb soft tissue defect.To design a sequential flap of thigh perforator to achieve primary closure of the anterolateral thigh free flap donor site.The area of anterolateral thigh free flap ranged from 9.0 cm×6.0 cm to 20.0 cm×1 1.0 cm,respectively.The area of thigh perforator flap ranged from 7.0 cm×3.0 cm to 12.0 cm×7.0 cm.Results All flaps healed uneventfully.Distal skin flap necrosis appeared in 1 flap which was healed after local transposition flap.All the patients were followed up for 1 to 6 months,with mean time of 4 months.The color,texture and appearance of flaps were satisfactory.Conclusion Sequential flap of thigh perforator,with reliable blood supply and constant position,represents an ideal model to close the anterolateral thigh free flap donor site of small defect.