Objective The molecular biology mechanism of cerebral ischemia animal models be analyzed from acupuncture experimental literatures by using data mining methods as a tentative exploration. Methods Retrieve the literatures of acupuncture animal experiments during 1978-2015, and screen the literatures about acupuncture effect on cerebral ischemia animal model of gene protein expression. Acupoint and gene/protein targets were extracted and normalized, and then the frequency statistics, association rule, calculation of complex networks were analyzed and visually displayed.Results A total of 350 papers were included. Seventy-nine acupoints were summarized, and the most frequent acupoint wasbaihui; A total of 180 genes/proteins were found, and the most frequent genes/proteins was BCL2. The most common group acuppointszusanli and quchifor the association rule results. The results of complex networks analysis showed the HSP70, BCL2, CASP3, Bax were more closely associated with cerebral ischemia injury or rehabilitation mechanism. Conclusions The mechanism of acupuncture treatment for cerebral ischemia was that acupuncture adjusting ischemic brain damage and improve the nervous system disorders from many channels and multiple targets.

Shear wave elastography (SWE) is used to quantitatively analyze the hardness of the tissue by Young's modulus.The hardness of the tissue is visualized in the form of color coding to distinguish the benign and malignant tissue detected.SWE has higher sensitivity,accuracy and specificity compared with traditional color doppler,which is more objective than elastography,safer,cheaper and simpler than MRI.SWE has a good application prospect in the diagnosis,clinical staging and curative effect monitoring of cervical cancer.

This paper was aimed to study the molecular mechanism of acupoint biology effect on acupuncture experiment articles.The acupuncture experiment articles in CNKI,Wanfang Data Resource Library and the China Biomedical Literature Database (CBM) were searched.The literature on acupoint and related genes were selected according to the literature inclusion criteria and exclusion criteria.The acupoint and corresponding genes in the literature were collected and analyzed by software written in Python,Cytoscape 3.3.0 and MECODE algorithm.The results showed that 2136 articles were collected,with 233 acupoints and 793 genes.Acupoints in the top 10 frequency were ST36 (Zusanli),GV20 (Baihui),PC6 (Neiguan),GV26 (Shuigou),GV14 (Dazhui),SP6 (Sanyinjiao),BL23 (Shenshu),LI11 (Quchi),GV16 (Fengfu) and GB34 (Yanglingquan).Genes in the top 10 frequency were BCL2,FOS,BDNF,Bax,CASP3,TNFA,GFAP,NGF,HSP70 and IL1B.Acupoint-groups in the top 5 frequency were GV14 (Dazhui)-GV20 (Baihui),GV26 (Shuigou)-GV20 (Baihui),GV20 (Baihui)-ST36 (Zusanli),SP6 (Sanyinjiao)-ST36 (Zusandi),PC6 (Neiguan)-GV26 (Shuigou).ST36 (Zusanli) was in the center of the acupoint-gene network.Through module analyzing,there were some genes belong to different pathways and some acupoints in one network module.It was concluded that ST36 (Zusanli) was the core acupoint in the acupoint experiment study,the stomach meridian of foot Yangming may be closely related with the metabolic pathway.This finding may provide new research ideas for clinical and experimental research.

Objective To investigate the effect of different uterine positions upon the dose distribution and target area conformability of organ at risk ( OAR) and explore the population suitable for 2-dimensional brachytherapy by comparing the dosimetry between CT-guided 3-and 2-dimensional brachytherapy for cervical cancer. Methods Thirty patients with cervical cancer received 72 cycles of 3-dimensional brachytherapy and then 2-dimensional brachytherapy was designed. The deviation angle of the uterus from the longitudinal asix on the coronal and sagittal CT images was measured. The obtained data were divided into the A to D and the group Ⅰ to Ⅳ according to the deviation angle ( T) of uterus position from the longitudinal axis on the sagittal CT images and the volume of HRCTV ( VHRCTV) to identify the optimal uterine position and range of VHRCTV for 2-dimensional brachytherapy. Statistical analysis was performed by paired t-test. Results The deviation angle of uterine position was not significantly correlated with the target CI index or D90 of HRCTV in both brachytherapy plans ( P value between 0. 077-0. 633 ) , whereas it was positively correlated with the D2 cm3 of bladder ( P value between 0. 001-0. 030) and negatively associated with the D2 cm3 of rectum in both 2-and 3-dimensional brachytherapy (P value between 0. 011-0. 016).In group B (|T|≤10°) and group Ⅲ(VHRCTVvalue between 86-96 cm3),the OAR parameters and CI index did not significantly differ between two brachytherapy plans ( P value between 0. 040-0. 463 ) , whereas varying degree of statistical differences was observed among other groups (P value between 0. 000-0. 940). Conclusions Although uterine position exerts no effect upon the conformal index of target area, it can affect the dose distribution of OAR. No statistical significance is noted in the dosimetry between 3-and 2-dimensional brachytherapy plans when the uterine position is almost flat (|T|≤10° ) and the radiotherapy target area is appropriate ( VHRCTVvalue between 86-96 cm3 ) . In this situation, 2-dimensional brachytherapy is the optimal option.

Objective:To evaluate the application of a medical image software (RadiAnt) in anatomical measurements and precision craniotomy via suboccipital retrosigmoid sinus approach.Methods:A total of 43 inpatients who underwent craniocerebral CT venography (CTV) in our hospital from June 2020 to June 2021 were selected for the study; the CTV data of 35 patients was used to measure the spatial relations between transverse sigmoid sinus junction (TSSJ) and asterion; the preoperative planning in suboccipital retrosigmoid sinus craniotomy with the software was performed in the left 8 patients. Craniotomy time (subjected to exposure of venous sinus margin), venous sinus injury and incidence of complications within 2 weeks of craniotomy in these 8 patients were recorded.Results:(1) Anatomic measurement: for the left side, TSSJ was located at (0.89±0.33) cm lateral and (0.63±0.46) cm inferior to the asterion, and their direct distance was (1.15±0.42) cm; TSSJ was located at (0.76±0.49) cm interior and (1.97±0.52) cm superior to the starting point of the mastoid notch, and their direct distance was (2.18±0.49) cm; about 29% asterion were located superior to the transverse sinus, 37% were located on the surface of the transverse sinus, and 34% were located inferior to the transverse sinus. For the right side, TSSJ was located at (0.88±0.39) cm lateral and (0.64±0.43) cm inferior to the asterion, and their direct distance was (1.12±0.54) cm; TSSJ was located at (0.74±0.40) cm interior and (1.93±0.45) cm superior to the starting point of the mastoid notch, and their direct distance was (2.16±0.43) cm; about 26% asterion were located superior to the transverse sinus, 40% were located on the surface of the transverse sinus, and 34% were located inferior to the transverse sinus. (2) Preoperative planning and surgeries: in these 8 patients, the key-hole was located at (0.96±0.49) cm lateral and (0.53±0.18) cm inferior to the asterion, and (0.46±0.35) cm interior and (1.76±0.47) superior to the starting point of mastoid notch. The interior of sigmoid sinus was located (0.13±0.51) cm interior and (0.21±0.46) cm superior to the starting point of mastoid notch. The inferior of the transverse sinus was located (2.17±0.45) cm interior and (0.53±0.35) cm inferior to the asterion. An accurate localization of the real position of TSSJ, inferior of transverse sinus and interior of sigmoid sinus was performed in all 8 surgical patients. The distance between the margin of the bone window and the interior of sigmoid sinus was (3.5±1.0) mm, and the craniotomy time was (25.7±4.1) min; no sinus injury was noted. Post-operative CT showed good reposition of the bone flaps and less bone defect. There was no cerebrospinal fluid leakage or subcutaneous effusion during the 2 weeks of follow-up.Conclusion:Anatomical measurements and preoperative planning can be quickly finished with low cost with Radiant ?, which provides an efficient and safe method for accurate craniotomy via suboccipital retrosigmoid approach.