1.A comparative evaluation of fracture resistance of endodontically treated teeth restored with different post core systems: an in-vitro study.
Chetana S MAKADE ; Ganesh K MESHRAM ; Manjusha WARHADPANDE ; Pravinkumar G PATIL
The Journal of Advanced Prosthodontics 2011;3(2):90-95
PURPOSE: To compare the fracture resistance and the mode of failure of endodontically treated teeth restored with different post-core systems. MATERIALS AND METHODS: Root canal treatment was performed on 40 maxillary incisors and the samples were divided into four groups of 10 each. For three experimental groups post space preparation was done and teeth were restored with cast post-core (Group B), stainless steel post with composite core (Group C) and glass fiber post with composite core using adhesive resin cement (Group D). Control group (A) samples were selected with intact coronal structure. All the samples were prepared for ideal abutment preparation. All the samples were subjected to a load of 0.5 mm/min at 130degrees until fracture occurred using the universal testing machine. The fracture resistance was measured and the data were analyzed statistically. The fracture above the embedded resin was considered to be favorable and the fracture below the level was considered as unfavorable. The statistical analysis of fracture resistance between different groups was carried out with t-test. For the mode of failure the statistical analysis was carried out by Kruskal-Wallis test and Chi-Square test. RESULTS: For experimental group Vs control group the fracture resistance values showed significant differences (P<.05). For the mode of failure the chi-square value is 16.1610, which means highly significant (P=.0009) statistically. CONCLUSION: Endodontically treated teeth without post core system showed the least fracture resistance demonstrating the need to reinforce the tooth. Stainless steel post with composite core showed the highest fracture resistance among all the experimental groups. Teeth restored with the Glass fiber post showed the most favorable fractures making them more amenable to the re-treatment.
Adhesives
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Collodion
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Dental Pulp Cavity
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Glass
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Incisor
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Resin Cements
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Stainless Steel
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Tooth
2.A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study.
Gautam Pyarelal BADOLE ; Manjusha Madhukar WARHADPANDE ; Ganesh Kothiramji MESHRAM ; Rakesh Namdeoraoji BAHADURE ; Shubha Gopal TAWANI ; Gopal TAWANI ; Shital Gautam BADOLE
Restorative Dentistry & Endodontics 2013;38(4):204-209
OBJECTIVES: The objective of this in vitro study was to evaluate and compare the cytotoxicity of four different root canal sealers i.e. Apexit Plus (Ivoclar Vivadent), Endomethasone N (Septodont), AH-26 (Dentsply) and Pulpdent Root Canal Sealer (Pulpdent), on a mouse fibroblast cell line (L929). MATERIALS AND METHODS: Thirty two discs for each sealer (5 mm in diameter and 2 mm in height) were fabricated in Teflon mould. The sealer extraction was made in cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM) using the ratio 1.25 cm2/mL between the surface of the sealer samples and the volume of medium in a shaker incubator. Extraction of each sealer was obtained at 24 hr, 7th day, 14th day, and one month of interval. These extracts were incubated with L929 cell line and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done. Two-way ANOVA for interaction effects between sealer and time and Post-hoc multiple comparison using Tukey's test across all the 16 different groups were used for statistical analysis. RESULTS: Apexit Plus root canal sealer was significantly less toxic than other sealers (p < 0.05) and showed higher cellular growth than control. Endomethasone N showed mild cytotoxicity. AH-26 showed severe toxicity which became mild after one month while Pulpdent Root Canal Sealer showed severe to moderate toxicity. CONCLUSIONS: Apexit Plus was relatively biocompatible sealer as compared to other three sealers which were cytotoxic at their initial stages, however, they became biocompatible with time.
Animals
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Bismuth
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Calcium Hydroxide
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Cell Culture Techniques
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Cell Line
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Dental Pulp Cavity*
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Dexamethasone
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Drug Combinations
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Epoxy Resins
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Fibroblasts
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Formaldehyde
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Hydrocortisone
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Incubators
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Mice
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Polytetrafluoroethylene
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Root Canal Filling Materials
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Silver
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Thymol
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Titanium