2.A large thymic mass with persistent active tissue in an elderly cadaver
Prabhas Ranjan TRIPATHY ; Binita CHAUDHARY ; Manisha Rajanand GAIKWAD ; Neethinathan Jacques BRITTO
Anatomy & Cell Biology 2019;52(1):93-96
Thymus is an encapsulated organ having its bilateral origin from the third pharyngeal pouch. It appears to be a single organ but actually it is bilobed. It attains its maximum development at puberty and then it begins to involute. The parenchyma is replaced by adipocytes and lymphocyte production declines. Here we present a large thymus with a small area of persistent active tissue in it which was obtained during routine undergraduate dissection class. Tissues taken from different quadrants of the large thymic mass were processed, embedded in paraffin and sections were taken for hematoxylin and eosin staining which showed presence of thymic tissue in only one quadrant. Further sections from that quadrant was treated with cytokeratin to confirm its epithelial origin. Therefore knowledge of a large persistent thymus will be helpful to the radiologists and surgeons for making differential diagnosis and in avoiding unnecessary surgical intervention.
Adipocytes
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Adolescent
;
Aged
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Cadaver
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Diagnosis, Differential
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Eosine Yellowish-(YS)
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Hematoxylin
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Humans
;
Immunohistochemistry
;
Keratins
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Lymphocytes
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Paraffin
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Puberty
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Surgeons
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Thymus Gland
3.A case of potentially lethal vascular variation in association with palmaris profundus muscle
Manisha Rajanand GAIKWAD ; Praveen Kumar RAVI ; Madhumita PATNAIK
Anatomy & Cell Biology 2019;52(3):349-353
Arterial variations in upper limbs are often reported commonly. Superficial arterial variations accounting for 4.2% of all arterial variations are hazardous during any invasive procedures of the upper limb, from routine intravenous injections to surgeries. Arterial variations are usually associated with inverted or absent palmaris longus. Palmaris profundus, a rare anomalous variation of palmaris longus has been reported in carpal tunnel syndrome as its tendon was associated with median nerve in the carpal tunnel. The authors reported a unique variation in the upper limb arterial pattern—the presence of bilateral superficial brachioulnar artery associated with unilateral palmaris profundus muscle and an abnormal radicle of musculocutaneous nerve to the median nerve in the left side.
Arteries
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Carpal Tunnel Syndrome
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Injections, Intravenous
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Median Nerve
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Musculocutaneous Nerve
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Tendons
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Upper Extremity
4.Cytological, histochemical, and ultrastructural study of human foetal liver of various gestation with future implications in segmental resection: an anatomical perspective
Arpan HALDAR ; Manisha Rajanand GAIKWAD ; Apurba PATRA ; Soumya C. BHATTACHARYA
Anatomy & Cell Biology 2022;55(1):92-99
The liver is the largest gland of the gastrointestinal tract having both exocrine and endocrine functions.Developmentally it arises as a ventral outgrowth from the gut endoderm during 3rd week of intrauterine life. The foetal liver is very important because of its synthetic and hemopoietic potential. The present work aimed to see the detailed histogenesis and development of the foetal liver by cytological, immunohistochemical and ultrastructural study. The liver tissue of nine aborted foetuses of various gestational age were studied. For cytology: special stains like Masson trichrome, periodic acid Schiff and reticulin were used, immunohistochemical staining was performed with triple antibodies (c-myc, Ki-67 and Ber-H2), and for ultrastructure: aluminium mounted specimens were coated with gold and argon gas and observed under scanning electron microscopy (EM). Cytology and immunohistochemistry showed the development of duct patterns and hemopoietic patterns in all stages of fetogenesis. The ductal plate was marked by the layer of dark brown staining cells at the edge of two portal tracts. The haemopoietic cells with sinusoids and aggregation of hepatocytes were observed in the early weeks of gestation. EM showed tree-like branching of a portal canal depicting hepatic segmentation of foetal liver.The organizational changes in lobular pattern, duct pattern, and microstructure of liver during fetogenesis are very crucial to achieve the adult morphology in feature. Histogenesis of the foetal liver follows a multistep process depending upon the gestational age, any deviation from normalcy may lead to structural and functional abnormality later in life.
5.Retrospective analysis of prevalent anatomy spotter's examination: an educational audit
Amit Purushottam TIRPUDE ; Manisha GAIKWAD ; Pradhnya Amit TIRPUDE ; Mantu JAIN ; Santanu BORA
Korean Journal of Medical Education 2019;31(2):115-124
PURPOSE: The spotter's (objectively structured practical examination) is an assessment tool which is widely used to test the practical knowledge of anatomy extensively. In Indian context it is used as an inclusive model in the gross anatomy practical examination and the marks allotted to it varies. The traditional spotter examination often has been blamed for only incorporating the initial levels of revised Bloom's taxonomy. This study retrospectively analysed the pre-professional spotter's examination score and tested its efficacy in terms of reliability, internal consistency, validity, educational impact, and resource intensiveness. METHODS: The summative data of student's score on four different days of unrepeated spotter's examination and final theory examination score was collected and tabulated. These four group of students acted as independent cohorts. The difficulty index (DI), point biserial correlation (PBC), Cronbach α (CA) and descriptive statistics of each cohort were calculated. Revised bloom taxonomy rating was applied to spotter's. RESULTS: We found heterogenous distribution of spotter's in each cohort according to DI. The PBCs and CA of each cohort were acceptable. The majority of spotter's tested the cognitive and comprehension domain of revised bloom's taxonomy. There was nonsignificant difference between mean scores of cohorts but we found low positive significant correlation between theory and spotter's score. CONCLUSION: We concluded that the construct of spotter's was reliable, internally consistent, had fair validity, variable educational impact, and sustainable resource intensiveness. We propose urgent redesigning of spotter's to include higher level of taxonomy to comply with emerging curricular changes.
Classification
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Cognition
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Cohort Studies
;
Comprehension
;
Curriculum
;
Educational Measurement
;
Humans
;
Retrospective Studies
6.Retrospective analysis of prevalent anatomy spotter's examination: an educational audit
Amit Purushottam TIRPUDE ; Manisha GAIKWAD ; Pradhnya Amit TIRPUDE ; Mantu JAIN ; Santanu BORA
Korean Journal of Medical Education 2019;31(2):115-124
PURPOSE:
The spotter's (objectively structured practical examination) is an assessment tool which is widely used to test the practical knowledge of anatomy extensively. In Indian context it is used as an inclusive model in the gross anatomy practical examination and the marks allotted to it varies. The traditional spotter examination often has been blamed for only incorporating the initial levels of revised Bloom's taxonomy. This study retrospectively analysed the pre-professional spotter's examination score and tested its efficacy in terms of reliability, internal consistency, validity, educational impact, and resource intensiveness.
METHODS:
The summative data of student's score on four different days of unrepeated spotter's examination and final theory examination score was collected and tabulated. These four group of students acted as independent cohorts. The difficulty index (DI), point biserial correlation (PBC), Cronbach α (CA) and descriptive statistics of each cohort were calculated. Revised bloom taxonomy rating was applied to spotter's.
RESULTS:
We found heterogenous distribution of spotter's in each cohort according to DI. The PBCs and CA of each cohort were acceptable. The majority of spotter's tested the cognitive and comprehension domain of revised bloom's taxonomy. There was nonsignificant difference between mean scores of cohorts but we found low positive significant correlation between theory and spotter's score.
CONCLUSION
We concluded that the construct of spotter's was reliable, internally consistent, had fair validity, variable educational impact, and sustainable resource intensiveness. We propose urgent redesigning of spotter's to include higher level of taxonomy to comply with emerging curricular changes.
7.Undescended cecum with accessory right colic artery: a rare case report.
Praveen Kumar RAVI ; Manisha Rajanand GAIKWAD ; Pravash Ranjan MISHRA ; Naina Santosh WAKODE ; Prabhas Ranjan TRIPATHY ; E Tripati PATRO ; Babita KUJUR ; Santosh Laxman WAKODE
Anatomy & Cell Biology 2017;50(3):242-244
Midgut malrotation and incomplete rotation are common causes of neonatal intestinal obstruction. At end of 10 week of intrauterine life, cecum will be placed in subhepatic region temporarily and descends to right lower quadrant by eleventh week. Arrest of cecum in subhepatic region or undescended cecum is a rare congenital anomaly of mid gut. Usually, it remains asymptomatic and is diagnosed incidentally. If any pathology occurs in anomalous part, like appendicitis then the diagnosis and treatment will be challenging in all age groups. Variation in blood supply have also been reported with anomalies leading to iatrogenic injuries during colonoscopy and surgeries. Lack of knowledge of these rare variations may lead to delayed diagnosis of appendicitis leading to perforation and surgical emergencies. In the present case, we describe an undescended cecum and its associated variation in branching pattern of superior mesenteric artery.
Appendicitis
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Arteries*
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Cecum*
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Colic*
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Colonoscopy
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Delayed Diagnosis
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Diagnosis
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Emergencies
;
Humans
;
Intestinal Obstruction
;
Mesenteric Artery, Superior
;
Pathology