Objective To analyze the volatile composition in the leaves of Sabina chinensis L. Ant. and Sabina chinensis L. Ant. cv. Kaizuca and to study their effects on bacteria. Methods GC-MS was employed in the analysis of volatile composition and four kinds of bacteria were used for testing the sterilization and bacteriostasis of the volatile oil. Results The main substance in volatile oil from the two kinds of plants, Sabina chinensis L. Ant. and Sabina chinensis L. Ant. cv. Kaizuca, was bornyl acetate and the percentage was 38.1% and 46.5% respectively. In addition, in the volatile oil from Sabina chinensis L. Ant. contained 24% of phellandrne and 12.4% of p-menth-l-en-4-o1, as for Sabina chinensis L. Ant. cv. Kaizuca, 30.0% of limonene and 7.9% of ?-pinene were contained. The volatile oil from Sabina chinensis L. Ant. had greater effects of bacteriostasis and sterilization on Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli compared with Sabina chinensis L. Ant. cv. Kaizuca. Conclusion Compared with Sabina chinensis L. Ant. cv. Kaizuca, the effects of sterilization and bacteriostasis of volatile oil from Sabina chinensis L. Ant. are much greater and with a larger spectrum of bacteria.

Objective To observe the effect of Rood therapy intervened very early on development of premature infants. Methods 148 hospital-born infants gestated 32 weeks with high risk of brain injury were divided into intervention group (n=74) and control group (n=74).All the cases accepted routine treatment and nursing, and the intervention group accepted Rood therapy in addition. They were followed up to 28th day, assessed with Neonatal Behavioral Neurological Assessment (NBNA). Results The NBNA score was more in the intervention group than in the control group (P<0.05) in all the sub-scores except primitive reflexes. Conclusion Rood therapy is useful to improve the neural development in premature infants in 32 to 36 weeks.

Objective ToexploretheMRIfeaturesofthepseudoaneurysmsandarteriovenousmalformationsinuterus,andtoimprove ourunderstandingonthemforhigherdiagnosticaccuracy.Methods TheMRIfeaturesofthe4casesofuterinepseudoaneurysmsand the7casesofuterinearteriovenousmalformationswhichdiagnosedandcuredbyourhospitalwereanalyzedretrospetively.Results TheMRIofuterinepseudoaneurysms(n＝4)shownodular(n＝3)oroval(n＝1)abnormalsignalinthemyometriumsofuterus,low signalintensityonT1WI,highsignalintensityonT2WI.Thelesionenhancementissimilartotheuterinearterycommunicatingwiththe lesion.TheMRIofuterinearteriovenousmalformations(n＝7)showuterinepatchyabnormalsignal,andtheirboundaryarenotclear. Thereareanumberoftortuousbloodvessels.Thelesionsareobviouslyheterogenouslyenhanced,andthethickeneddrainagevessels couldalsobeseenoncontrastGenhancedimages.Thelesionsofuterinepseudoaneurysmsandarteriovenousmalformationsdon’tshow highsignalintensityonDWI,suggsetingnolimiteddiffusion.Conclusion The MRIcharacteristicsoftheuterinepseudoaneurysms showthattheuterinelesionenhancementissimilartotheuterinearterycommunicatingwiththelesion.The MRIcharacteristicsof theuterinearteriovenous malformationsarethatthereareanumberoftortuousbloodvesselsanddrainagevesselsintheuterus. Therearen’tlimiteddiffusioninthesetwodiseasesonDWI.

Objective:To investigate the protective effect and possible mechanism of sulforaphane (SFN) on acute liver injury in mice induced by diquat (DQ) poisoning.Methods:Forty-eight male C57BL/6 mice were divided into Control group, DQ model group (DQ group), SFN intervention group (DQ+SFN group), and SFN control group (SFN group) using a random number table method, with 12 mice in each group. Acute liver injury mice model was established by one-time intraperitoneal injection of 1 mL of 40 mg/kg DQ solution at once. SFN group was injected with 1 mL of ddH 2O. After 4 hours of molding, 0.5 mL of 5 mg/kg SFN solution was injected into the peritoneal cavity of the DQ+SFN group and SFN group, once daily for 7 consecutive days. DQ group and Control group were injected with an equal amount of ddH 2O. Then, the mice were euthanized to collect liver tissue and blood samples, and the levels of plasma biomarkers alanine aminotransferase (ALT) and aspartate aminotransferase (AST), as well as oxidative stress indicators such as superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA) in liver tissue were measured. The changes of liver structure were observed under transmission electron microscopy. The apoptosis and reactive oxygen species (ROS) level in liver tissue were observed under fluorescence microscope. Western blotting was used to detect the protein expressions of nuclear factor E2-related factor 2 (Nrf2), hemeoxygenase-1 (HO-1), Kelch-like ECH-associated protein 1 (Keap1), and cleaved caspase-9 in liver tissue. Results:Compared with the Control group, the liver mitochondria in the DQ group showed severe swelling, partial dissolution of the matrix, and cristae rupture and loss; the levels of plasma AST and ALT significantly increased, the MDA content in the liver increased, the activities of SOD and GSH decreased, the level of ROS significantly increased, the number of apoptotic cells in the liver significantly increased, the protein expressions of Nrf2 and HO-1 significantly decreased, and the protein expressions of Keap1 and cleaved caspase-9 significantly increased. Compared with the DQ group, the mitochondrial damage in the DQ+SFN group was reduced, the levels of plasma AST and ALT were significantly reduced [ALT (U/L): 58.22±4.39 vs. 79.94±3.32, AST (U/L): 177.64±8.40 vs. 219.62±11.60, both P < 0.01], the liver MDA content decreased, and the activities of SOD and GSH increased [MDA (μmol/g: 5.63±0.18 vs. 5.96±0.29, SOD (kU/g): 102.05±4.01 vs. 84.34±5.34, GSH (mmol/g): 16.32±1.40 vs. 13.12±1.84, all P < 0.05], the production of ROS in liver tissue was significantly reduced [ROS (fluorescence intensity): 115.90±10.89 vs. 190.70±10.16, P < 0.05], and apoptotic cells were significantly reduced (cell apoptosis index: 4.39±1.00 vs. 10.71±0.56, P < 0.01), the protein expressions of Nrf2 and HO-1 were significantly increased, while the protein expressions of Keap1 and cleaved caspase-9 were significantly decreased (Nrf2/β-actin: 1.15±0.04 vs. 0.93±0.05, HO-1/β-actin: 1.75±0.12 vs. 0.78±0.04, Keap1/β-actin: 1.00±0.14 vs. 1.28±0.13, cleaved caspase-9/β-actin: 1.31±0.12 vs. 1.81±0.09, all P < 0.05). However, there was no statistically significant difference in various indicators between the SFN group and the Control group. Conclusion:SFN can activate the Keap1/Nrf2 signaling pathway to alleviate DQ induced acute liver injury in mice.