1.Application of Doppler combined with flap island on monitoring the post-operative blood supply of buried free flap.
Meiai XU ; Xiaohua JIANG ; Mang XIAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2013;27(24):1363-1365
OBJECTIVE:
To explore the feasible monitoring techniques for the buried free flap.
METHOD:
Buried free flaps in reconstruction were monitored using combined monitoring techniques in five cases of advanced hypopharyngeal cancer The monitoring effects were analyzed retrospectively.
RESULT:
The operations were carried out successfully and all the five patients got primary healing of incision without vascular crisis. Four patients need combined monitoring techniques to confirm the microcirculatory results of the buried free flap.
CONCLUSION
Application of Doppler combined with flap island was feasible on monitoring the blood supply of post-operative buried free flap, it is a good monitoring technique.
Aged
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Carcinoma, Squamous Cell
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diagnostic imaging
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pathology
;
surgery
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Free Tissue Flaps
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blood supply
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Head and Neck Neoplasms
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diagnostic imaging
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pathology
;
surgery
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Humans
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Hypopharyngeal Neoplasms
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diagnostic imaging
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pathology
;
surgery
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Hypopharynx
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Male
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Middle Aged
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Postoperative Period
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Retrospective Studies
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Squamous Cell Carcinoma of Head and Neck
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Surgical Flaps
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blood supply
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Ultrasonography, Doppler
2.Myeloidleukemia with external auditory canal granulocytic sarcoma: a case report.
Xiao WANG ; Rongjun MANG ; Yuan ZHOU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(1):91-93
We describe a 44-year old man who suffered an isolated external auditory canal granulocytic sarcoma in remission of acute myeloid leukemia. The patient confirmed acute promyelocytic leukemia three years ago and the disease was in remission after treatment. Two months ago he presented pain and hearing loss in the left ear and the symptom developed gradually. At otoscopic examination a tumoral lesion was noted in the external auditory canal and computerized tomography scan showed a mass in the left external acoustic meatus without bone erosion.
Adult
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Ear Canal
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diagnostic imaging
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pathology
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Hearing Loss
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Humans
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Leukemia, Promyelocytic, Acute
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Male
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Sarcoma, Myeloid
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diagnostic imaging
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pathology
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Tomography, X-Ray Computed
4.Culture and identification of mouse myeloid semimature dendritic cells.
Bi-mang FU ; Xiao-shun HE ; Si YU ; An-bin HU ; Yi MA ; Jie-fu HUANG
Acta Academiae Medicinae Sinicae 2008;30(4):430-435
OBJECTIVETo investigate the methods of culturing and identifying mouse myeloid semimature dendritic cell (smDC) in vitro.
METHODSMyeloid monocytes derived from 6-week-old C57 BL/6 mice were cultured in RPMI-1640 medium containing 10% fetal bovine serum, 2 ng/ml recombinant murine granulocyte macrophage-colony stimulating factor (GM-CSF), and 20 ng/ml recombinant murine interleukin (IL)-4 for 9 days. Then cells were incubated with 40 ng/ml tumor necrosis factor-alpha (TNF-alpha) for 24 hours to obtain smDC. Meanwhile, smDC was differentiated into mature dendritic cell (mDC) or immature dendritic cell (iDC) by treatment with 1 micro/m1 lipopolysaccharide (LPS) or without LPS. The morphological features of smDC were assayed by inverted microscopy and scanning electron microscopy. Surface markers such as CD11c, CD4O, CD8O, CD86, and MHC-II were tested by flow cytometry. IL-1beta, IL-6, IL-12, and IL-10 in the supernatant were tested by ELISA. The activation of allogene lymphocyte (BALB/c mice) stimulated by C57BL/6 myeloid smDC in mixed lymphocyte reaction was examined by Cell Counting Kit-8 in vitro.
RESULTSThe shape of smDC was round or oval-shaped, and the diameter of smDC was about 15 microm. The length of smDC dendrite was between 5 to 10 microm. smDC, iDC, and mDC all expressed high level of CD11 c. The expressions of MHC-II, CD40, CD80, and CD86 on smDC were higher than those of iDC and lower than those of mDC. IL-1beta, IL-6, and IL-12 secretion of smDC was significantly lower than that of mDC (P < 0.01), and IL-12 was significantly lower than that of iDC (P < 0.05), while no significant difference of IL-1beta and IL-6 secretion was found between smDC and iDC (P > 0.05). Furthermore, IL-10 secretion was not significantly different among these three kinds of DCs (P > 0.05). The effect of allogene lymphocytes activation on smDC was significantly lower than that of mDC and positive control (P < 0.01), but had no significant difference when compared with that of iDC and negative control (P > 0.05).
CONCLUSIONSsmDC may be a relatively independent dendritic cell sub-population in terms of function and morphology. It is a feasible way to induce myeloid monocytes to differentiate into smDC using GM-CSF, IL-4, and TNF-alpha in vitro.
Animals ; Cell Culture Techniques ; Cell Differentiation ; Cells, Cultured ; Cytokines ; immunology ; Dendritic Cells ; cytology ; immunology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Monocytes ; cytology ; immunology
5.Effect of the new human transcription factor hBKLF on the proliferation, differentiation of K562 cell line and hemoglobin synthesis.
Mang-Ju WANG ; Xiao-Yun MA ; Yong-Jin SHI ; Shu-Lan WU ; Fu-Chu HE
Journal of Experimental Hematology 2006;14(6):1083-1088
The human basic Krüppel-like factor (hBKLF) is a newly cloned human transcription factor from the cDNA library of fetal liver. It belongs to the Krüppel-like transcription factor family. Previous expression study showed that it is a hematopoietic related factor. This study was aimed to investigate the effect of hBKLF on cell proliferation, differentiation and hemoglobin synthesis by using K562 cell line as model. The sense and antisense expression plasmids of hBKLF were constructed, and transfected into K562 cells by lipofectamine. After G418 selection for 4 weeks, the cell line with stable expression of the gene was obtained. Then the hBKLF expression level, proliferation ability, colony formation and hemoglobin production were detected by RT-PCR and Western blot, MTT method, methyl cellulose semisolid culture method and benzidine test respectively. The morphologic change of cell was observed with inverted microscope. The results showed that the sense plasmid could increase hBKLF level and antisense plasmid could decrease hBKLF expression. When hBKLF level was down-regulated, K562 cells could proliferate more quickly and synthesize more hemoglobin. But there were no differences in colony formation ability and no apparent morphologic change. It is concluded that hBKLF can inhibit hematopoietic cell proliferation and hemoglobin synthesis. It is suggested that hBKLF plays an important role in the proliferation and differentiation of hematopoietic cells.
Animals
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COS Cells
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Cell Differentiation
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physiology
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Cell Proliferation
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drug effects
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Cell Transformation, Neoplastic
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drug effects
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Cercopithecus aethiops
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Hemoglobins
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biosynthesis
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Humans
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K562 Cells
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Kruppel-Like Transcription Factors
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biosynthesis
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genetics
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pharmacology
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Transcription Factors
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biosynthesis
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genetics
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Transfection
6.Experience of endovenous radiofrequency combined with TriVex in treatment of chronic venous insufficiency in lower extremity.
Shao-Mang LIN ; Zhi-Hui ZHANG ; Yan-Dan YAO ; Jian-Bin XIAO
Chinese Journal of Surgery 2009;47(4):271-274
OBJECTIVETo evaluate therapeutic results of endovenous radiofrequency in combination with TriVex in treatment of venous insufficiency in lower extremities.
METHODSOne hundred and fifty patients with chronic venous insufficiency (150 limbs) were randomly assigned to Group A (75 limbs) and Group B (75 limbs). Patients in Group A were treated with long saphenous veins radiofrequency ablation procedures in combination with TriVex. Patients in Group B were treated with long saphenous veins traditional stripping operation in combination with TriVex. The postoperative pain, average hospital stay and short-term results in hospital were compared between the two groups. Self-assessment of the operation 4 weeks after, changes of CEAP classification, venous clinical severity score (VCSS) and chronic venous insufficiency questionnaire (CIVIQ) score were compared between the two groups.
RESULTSThe operation time in Group A was (67 +/- 11) min, compared with (59 +/- 9) min in Group B (P > 0.05). Postoperative pain and average hospital stay in Group A were significantly lower than those in Group B (P < 0.05). The scores of self-assessment of the operation in Group A was higher than that in Group B 4 weeks after operation (P < 0.05). The change of CEAP classification, VCSS and quality of life were significant after operation in both groups. The VCSS of Group A decreased by 4.6 +/- 2.5 compared with 4.3 +/- 2.7 in Group B (P > 0.05).
CONCLUSIONSEndovenous radiofrequency combined with TriVex for treatment of venous insufficiency in lower extremity is available, effective and with less trauma and faster recovery. CEAP classification, VCSS and CIVIQ are useful tools for assessing outcomes after radiofrequency in these patients.
Adult ; Aged ; Aged, 80 and over ; Catheter Ablation ; Female ; Follow-Up Studies ; Humans ; Lower Extremity ; blood supply ; Male ; Middle Aged ; Minimally Invasive Surgical Procedures ; Saphenous Vein ; surgery ; Treatment Outcome ; Venous Insufficiency ; surgery
7.Analysis of the efficacy of three radical mastoidectomy surgery treatment of cholesteatoma otitis media and explore treatment measures for cholesteatoma recurrence after surgery
Junwu WU ; Mang XIAO ; Yunfei JIN
China Modern Doctor 2015;(2):62-64,67
Objective To compare clinical efficacy of open radical mastoidectomy (ORM) and complete bi-style radical mastoid surgery (CRMS) and open radical mastoidectomy-mastoid tamponade(ORM-MT) treatment of cholesteatoma otitis media (COM), and explore treatment measures for cholesteatoma recurrence after surgery. Methods The 130 cases of COM patients were randomly divided into ORM group(46 cases), CRMS group (38 cases) and ORM-MT group (46 cases). The values of average air conduction hearing threshold value and Air-bone difference and recurrence rate during fol-low-up were compared. Results The values of average air conduction hearing threshold value and Air-bone difference in CRMS group after three months were significantly lower than ORM group (q=12.055, 7.761, P<0.01), the values of average air conduction hearing threshold value and Air-bone difference in CRMS group after three months were signifi-cantly lower than ORM group (q=8.764, 11.443, P<0.01). But CRMS group and ORM-MT group had no significant dif-ference (q=1.170, 0.575, P>0.05). Recurrence rate of ORM, CRMS and ORM-MT group was 5 ears (10.9%), 9 ears (23.7%) and 4 ears (8.7%), respectively. Recurrence rate of CRMS group was significantly higher than ORM group and ORM-MT group (χ2=6.640, 9.072, P<0.05). Conclusion CRMS and ORM-MT technique is superior in improving hear-ing function, but CRMS has a higher recurrence rate. Face higher neural crest and poor drainage patients should be performed radical mastoid surgery again.
8.Fibrotic remodeling of nasal mucosa in rat model after radiation injury.
Mang XIAO ; Jian-Guo TANG ; Xiao-Nan SUN ; Xiu-Wen JIANG ; Bao-Zhen LUO ; Li-Na ZHAO
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2009;44(2):130-135
OBJECTIVETo investigate the role of nasal mucosa fibrosis on radiation induced nasal mucosa injury.
METHODSSeventy two male rats were randomly divided into two groups, control group and irradiation injured group (radiation dose were 40 Gy); the rats were killed 1 week, 2 weeks, 4 weeks, 8 weeks, 3 months and 6 months after the finish of radiation. The middle turbinates of the animals were removed. The pathological change of the nasal mucosa were observed with scanning electron microscope, transmission electron microscope, hematoxylin and eosin (HE), alcian blue-periodic acid-Schif (AB-PAS), and Masson Trichrome (MT). The Hyp content in nasal mucosa was measured with chemo-chromatometry.
RESULTSAfter radiation, the pathological characteristics in early stage (within 4 weeks) was acute inflammatory reaction. The repair of nasal mucosa started 4 weeks after radiation, lasted to 6 months. The deposition of collagen in nasal mucosa could be found 1 week after irradiation and increased gradually.
CONCLUSIONIrradiation could induce a serials of pathological changes on nasal mucosa. The nasal mucosa fibrosis may be one of the reasons of persistent irradiation induced nasal mucosa injury.
Animals ; Fibrosis ; Male ; Nasal Mucosa ; pathology ; Radiation Injuries, Experimental ; pathology ; Rats ; Rats, Sprague-Dawley ; Wound Healing
9.The study of pedicle imaging and reformation with the multi-slice spiral CT in the surgical treatment of lumbar spondylolisthesis.
Xiao-Ping LUO ; Hong-Lin TENG ; Rong-Hua CHEN ; Yu-Xiang ZHAO ; Xin-Mang WENG ; Wei-Jian CHEN ; Wei-Wei YIN
China Journal of Orthopaedics and Traumatology 2011;24(4):299-303
OBJECTIVETo evaluate the value of pedicle parameter obtained by the reformation images on multi-slice spiral CT (MSCT) in the surgical treatment of lumbar spondylolisthesis.
METHODSFrom January 2009 to March 2010, 60 patients with lumbar spondylolisthesis failing in conservative treatment were enrolled into the study and divided into experimental and control group randomly (each group with 30 patients). There were 26 males and 34 females ranging in age from 18 to 59 years with an average of (42.60 +/- 9.36) years. The experimental group was examined with volumetric scanning on MSCT before operation. Reformation such as multiplanar reconstruction (MPR) and volume rendering (VR) were carried out at the work station. Transverse section angle (TSA), sagittal section angle (SSA), pedicle length (PL), pedicle width (PW) and pedicle height (PH) were measured on different images and pedicle screws were implanted according pedicle parameter. In control group, the pedicle screws were implanted according to conventional anatomic landmark. Preparative time of screw canal and accuracy of screw were compared between two groups.
RESULTSA hundred fifty-six screws were inserted in experiment group,143 screws were excellent, 11 good, and 2 poor. A hundred fifty screws were inserted in control group, 101 screws were excellent, 26 good, and 23 poor. There was significant difference in accuracy of screw between two groups (P < 0.001). The preparative time of screw canal in experiment group was (66.20 +/- 7.31) s, and was shorter than that of control group [(104.11 +/- 9.51) s, P < 0.001)].
CONCLUSIONAbundant information and parameter could be obtained with the MSCT reconstruction images. The images and parameters could make a perfect operative strategy before operation, adjust the direction of pedicle screws during operation, avoid and decrease operative complications effectively.
Adolescent ; Adult ; Bone Screws ; Female ; Humans ; Lumbar Vertebrae ; diagnostic imaging ; surgery ; Male ; Middle Aged ; Spondylolisthesis ; diagnostic imaging ; surgery ; Tomography, Spiral Computed ; methods
10.Investigation on role of p38α mitogen-activated protein kinases in human esophageal squamous cell carcinoma cell line Eca109.
Shu-tao ZHENG ; Tao LIU ; Qing LIU ; Mang LU ; Xiang-peng GAO ; Iiyar SHEYHIDIN ; Ren-yong LIN ; Xiao-mei LU
Chinese Journal of Preventive Medicine 2013;47(8):757-761
OBJECTIVETo investigate the role of p38α mitogen-activated protein kinases (MAPK) in human esophageal squamous cell carcinoma cell line Eca109.
METHODSSpecific short hairpin (shRNA) vector as well as eukaryotic expression vector harbouring full length cDNA of human p38α MAPK were transfected into Eca109 cells. Cell proliferation after transfection was detected by MTT, cell cycle and apoptosis were assayed by flow cytometry. The variation of migration and invasion after transfection was determined using wound healing assay and Transwell assay, respectively.
RESULTSThe proliferation of Eca109 cells after knock-down for 48 h (0.951 ± 0.086) was significantly increased (t = 3.20, P < 0.05) compared with control (0.811 ± 0.012), Sphase was increased but not significantly. Cell apoptosis rate after knock down for 48 h (17.400 ± 5.495) was significantly increased (t = 40.06, P < 0.01) compared with control(1.000 ± 0.721) . Migration after knock down for 72 h (0.034 ± 0.031) were enhanced pronouncedly (t = -5.79, P < 0.01) compared with control (0.278 ± 0.021) and invasive ability also increased; whereas the proliferation of Eca109 cells after over-expression for 48 h (0.472 ± 0.089) was inhibited significantly (t = -7.50, P < 0.01) compared with control(0.811 ± 0.012), cells arrested at G1 phase (t = 4.80, P < 0.01). Cell apoptosis rate (32.233 ± 1.457) were decreased significantly (t = 17.20, P < 0.01) compared with control (1.000 ± 0.721) mm, migration after overexpression for 72 h ((0.770 ± 0.054) mm) was suppressed pronouncedly compared with control groups of (0.278 ± 0.021) mm(t = 11.00, P < 0.01).Invasion after overexpression was inhibited.
CONCLUSIONSp38α MAPK plays an anti-oncogenic role in the pathogenesis of esophageal squamous cell carcinoma cell line Eca109.
Carcinoma, Squamous Cell ; genetics ; metabolism ; pathology ; Cell Division ; Cell Line, Tumor ; Cell Proliferation ; Esophageal Neoplasms ; genetics ; metabolism ; pathology ; Humans ; Mitogen-Activated Protein Kinase 14 ; metabolism ; RNA, Small Interfering ; Transfection