The purpose of this prospective study was to examine the injury characteristics in collegiate women’s lacrosse players. Injury data were collected from the Division I collegiate women’s lacrosse team for a 2-year. The total athlete-hours and athlete-exposures over the 2-year period were 27,621 and 13,437, respectively. The total number of injury was 309 injuries, and the total incidence rate was 11.19 injuries (95% confidence interval, 9.94 — 12.43) per 1000 athlete-hours and 23.00 injuries (20.43 — 25.56) per 1000 athlete-exposures, respectively. The most frequent location of injury was the ankle, followed by the knee and the thigh (20.1%, 14.6%, and 10.7% of all injuries). The proportion of lower extremity injuries accounted for 73.5% of all injuries. The most common types of injury was sprains and muscle cramps/spasms (24.9% and 24.6%, respectively). Overuse, contact (e.g., other players, the cross, and the ball), and non-contact injury was the 3 most common cause of injury (39.1%, 24.9%, and 17.2%, respectively). The primary injury characteristics of collegiate women’s lacrosse players were non-contact ankle sprain. Injury recurrence accounted for 26.1% of all injuries, and the most common period required for the player to return to practice and/or games was between 3 and 7 days. Midfield position player suffered the most number of injuries compared to other position player. An understanding of the injury characteristics of women’s lacrosse players may facilitate the development of the comprehensive strategy for injury prevention which is recommended by the sports medicine community.