1.Roles of UBE2C in breast cancer and its clinical significance
Journal of International Oncology 2015;(10):766-768
Ubiquitin-conjugating enzyme 2C (UBE2C)is involved in cell cycle progression,mitosis regulation and targeted degradation of short-lived proteins.UBE2C is the cancer-related ubiquitin-conjugating enzyme,which is significantly higher in breast cancer tissues compared to the adjacent normal tissue.The abnormal expression of UBE2C participates in the process of occurrence,development and metastasis of breast cancer by the regulation of cell cycle progression,C-erbB-2,cell apoptosis factors and metastasis related genes. It will provide reference for the diagnosis and prognosis of breast cancer.
2.Effects of mineral trioxide aggregate on the proliferation and differentiation of human pulp cells from primary and permanent teeth.
Minyong WANG ; He LIU ; Shenglin LI ; Man QIN
West China Journal of Stomatology 2015;33(1):75-79
OBJECTIVEThis study aims to investigate the effects of mineral trioxide aggregate (MTA) and calcium hydroxide on proliferation and differentiation of human pulp cells from primary and permanent teeth.
METHODSCell proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. The mRNA expression levels of dentinogenesis-related factors, including alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), and odontoclastogenesis-related factors, such as osteo- protegerin (OPG) and receptor activator of NF-κB ligand (RANKL), were determined by real time polymerase chain reac- tion (PCR).
RESULTSPrimary and permanent pulp cells treated with calcium hydroxide exhibited significantly lower proli- feration rates than the control cells (P<0.01). By contrast, the MTA-treated group showed significantly higher proliferation rates than the control group (P<0.01). Real time PCR results showed that calcium hydroxide-treated primary pulp cells exhi- bited significantly decreased ALP, DSPP, and OPG expression compared with the control group (P<0.01). Conversely, the MTA-treated group displayed significantly increased ALP, DSPP, and OPG expression (P<0.01). Calcium hydroxide-treated primary pulp cells also exhibited significantly upregulated RANKL expression (P < 0.01); by contrast, MTA-treated cells did not show any change in RANKL expression (P>0.05). Likewise, MTA-treated permanent pulp cells showed significantly upregulated ALP and DSPP expression (P < 0.01). However, the calcium hydroxide-treated group remained almost the same as the control group (P > 0.05). Neither MTA nor calcium hydroxide affected OPG and RANKL expression in per- manent pulp cells (P > 0.05).
CONCLUSIONMTA is more suitable as a pulp-capping agent, particularly in primary teeth, than calcium hydroxide.
Aluminum Compounds ; Calcium Compounds ; Calcium Hydroxide ; Cell Differentiation ; Cell Proliferation ; Dental Pulp ; Dentition, Permanent ; Drug Combinations ; Extracellular Matrix Proteins ; Humans ; Oxides ; Phosphoproteins ; Sialoglycoproteins ; Silicates
4.CD23 and CD19 expression on peripheral blood lymphocytes in asthmatic children.
Yong-qin ZHU ; Man-li KANG ; Zhi-min CHEN
Chinese Journal of Pediatrics 2003;41(7):554-554
Antigens, CD19
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analysis
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Asthma
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blood
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Child
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Child, Preschool
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Female
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Flow Cytometry
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Humans
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Immunoglobulin E
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blood
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Lymphocytes
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immunology
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Male
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Receptors, IgE
;
analysis
5.One new glycoside naphthopyranone from the Yiling cave-derived Metarhizium anisopliae NHC-M3-2
Li-man ZHOU ; Yi HAO ; Ju-xiang MENG ; Fang-fang QIN ; Qing-hua QIN ; Cong WANG ; Fan-dong KONG
Acta Pharmaceutica Sinica 2023;57(10):3076-3081
Seven compounds were isolated from fermentation extract of cave-derived
6.Role of astrocytes in sensory processing in central nervous system.
Journal of Zhejiang University. Medical sciences 2011;40(6):673-679
There are two types of cells in the central nervous systems (CNS) of mammals-neurons and glia. The structure and function of neurons have been thoroughly studied; while the role of glia in information processing has not been systematically studied because they cannot produce action potentials like neuron. During the past decades, glial cells were considered to play a supportive role in CNS instead of information processing. Recently, a variety of studies suggest that glial cells are actively involved in the regulation of brain function associated with neurons. Glial cells, especially astrocytes play important roles in different sensory processing. In the present article, we review the role of astrocytes in sensory processing in the CNS.
Animals
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Astrocytes
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cytology
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physiology
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Central Nervous System
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physiology
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Humans
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Sensation
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physiology
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Synapses
;
physiology
7.Safety issues related to fine needle aspiration cytology of thyroid nodules.
Yu-qi YAO ; Xia YANG ; Sheng QIN ; Ji-man LI ; Hong YANG
Chinese Journal of Pathology 2012;41(1):48-49
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Biopsy, Fine-Needle
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adverse effects
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methods
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Child
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Diagnosis, Differential
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Diagnostic Errors
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Female
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Hematoma
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etiology
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Humans
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Male
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Middle Aged
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Thyroid Neoplasms
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diagnosis
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pathology
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Thyroid Nodule
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pathology
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Young Adult
8.Telmisartan Prevent Oxidative Injuries in Endothelial Cells Induced by High Glucose
Jun HUANG ; Jing XIAO ; Lian-Man HE ; Hai-Yan QIN ; De-Qiang LI ;
Chinese Journal of Hypertension 2007;0(04):-
Objective To investigate the effect of telmisartan on the oxidative stress induced by high glucose in human umbilical vein endothelial cell (HUVEC) in vitro.Methods HUVEC were cocultured with telmisartan (1?10~(-6) mol/L) and various concentration of glucose(5,30 mmol/L) for 0,12,24,36,48 h respectively. The level of MDA in the supernatants of cultured endothelial cells was measured by thiobarbituric acid test,SOD was determined by xanthine oxidase test.The protein expression of peroxisome proliferator activated receptors ? (PPAR-?) in HUVEC 24 h was assessed by Western blot after treatments.Results High glucose significantly increase the levels of MDA (before:1.2?0.06 vs after:1.6?0.1 mmol/mL,P
10.Frequency spectrum analysis of dark-adapted oscillatory potentials in normal rats
Jia-man, DAI ; Ying-di, CHEN ; Shi-ying, LI ; Zheng-qin, YIN ; Chuan-huang, WENG
Chinese Journal of Experimental Ophthalmology 2012;(10):919-921
Background Oscillatory potentials (OPs) of scotopic electroretinogram (ERG) plays an important role in the evaluation of visual function in multiple retinal diseases.However,the origin of OPs is uncompletely clear.It is essential to analyze the time domain and frequency domain components for the further study of OPs.Objective The present study was to investigate the change characteristics of frequency spectrum of scotopic OPs with age and stimulating intensity.Methods RCS-rdy+-p+rats with the ages of 21,25,32,35,37,46,60,90 days were selected iu this study and 3 rats for each.Scotopic flash ERG were recorded from all the rats with RETI-scan system.Gold-foil ring cornea recording electrode was used as the recording electrode and the steel needle electrode was used as the reference and earth electrode during the record.The intensity of stimulating light was set at-20,-10,-5,0 and 5 dB respectively.Data were output into the computer and processed by the software Matlab7.0.Results The principle frequency corresponding to maximum amplitude component was 80-120 Hz in the various ages of rats under the different stimulating conditions above.With the increase of the intensity of stimulating light,high frequency component (200-250 Hz) began to appear and the amplitudes showed a gradually raise upon the intensity of light.The major component was subdivided into two peaks at 0 dB stimulation.Further,the age affected the major frequency peak with the maximum value at 60-day-old rats and the minimum value at 25-day-old rats.Also,the pass-band width of main amplitude appeared to be maximal at 60-day-old rats and minimal at 25-day-old rats.Conclusions OPs in Rcsrdy+-p+ rats are influenced by stimulating intensity and agc.Stimulating intensity affects the amplitude and age lead to the change of distribution of primary frequency of OPs.It is possible to know the influences of the degeneration of rods and be helpful to diagnosis this kind of disease.