Cyclin-Dependent Kinase 6 ; metabolism ; Gene Expression Regulation, Neoplastic ; Genes, p53 ; Humans ; Killer Cells, Natural ; pathology ; Lymphoma, T-Cell ; genetics ; metabolism ; pathology ; Mutation ; Nose Neoplasms ; genetics ; metabolism ; pathology ; Protein Tyrosine Phosphatase, Non-Receptor Type 6 ; Proto-Oncogene Proteins c-kit ; metabolism ; fas Receptor ; genetics ; metabolism

Objective To describe the status quo of hope among nurses in tertiary general hospitals and to explore its influencing factors. Methods A cross-sectional survey was conducted. The Hope Status Scale, the Nurse′s Job Stressor Scale and self-designed demographic questionnaire were delivered to 1 160 nurses from nine hospitals. Results The score of hope scale was 23.05 ± 6.16. Different gender (t=2.045, P=0.004), age (F=7.193, P=0.000), marital status (t=2.806, P=0.005), length of employment (F=11.421, P=0.000), department (F=2.394, P=0.015), monthly income (F=5.351, P=0.001), participate in extracurricular activities (F=37.448, P=0.000) reached a statistically significant level. A significantly negative correlation was found between hope and work stress of nurses (r=-0.233, P=0.000);Logistic regression results showed that participating in extracurricular activities, length of employment, monthly income, departments and job stressors were major influencing factors of hope state of nurses. Conclusions More attention should be paid to the improvement of nurse′s hope. According to the influencing factors of hope, measures should be taken to strengthen the level of hope.

Objective To evaluate the features of cervical lesions on real-time elastography.Methods The elastograms of cervical lesions were analyzed and all of them were evaluated by improved elastographic strain scores,which were classified into 5,and by strain ratio,with pathologic analysis as the reference standard.Results The elasticity scores of all normal cervixes were 1.The score of 95 ％ of benign lesions was not more than 2,the strain ratios of benign lesions were 0.75 ± 0.32.The score of 90％ of the cervical cancer was not less than 3,the strain ratios of 38 malignant lesions were 3.31 ± 1.47.There were significant difference between benign lesions and malignant lesions in elastic scores and elastic strain ratios (P ＜0.001).Conclusions There are some typical features of different cervical lesions on elastography.

Objective To compare the clinical efficacy of different doses of ultrafine extracted granule preparation (EGP) and traditional herbal decoction (THD) of Li Zhong Tang in treatment of epigastric pain. Methods Sixty cases of epigastric pain patients in accordance with the diagnostic criteria of deficiency and cold pattern of spleen and stomach in TCM were randomly divided into THD group, 1/3 dose group and 1/5 dose group, and were given THD, 1/3 dose of ultrafine EGP and 1/5 dose of ultrafine EGP, respectively. The clinical efficacy of the three groups after one course of medication was comparatively analyzed. Results There were no significant differences in age, course of disease, symptom score before treatment, epigastric pain efficacy and syndrome curative effect among the three groups, the differences had no statistical significance (P>0.05). The severity, frequency and duration of epigastric pain were all reduced in the three groups, with significant differences between before and after treatment (P<0.05). Conclusion There are no significant differences in the clinical efficacy on epigastric pain among THD, 1/3 dose of ultrafine EGP and 1/5 dose of ultrafine EGP. In addition, the effect of 1/3 dose group is very close to the THD group.

Objective To explore the application of mini-clinical evaluation exercise (Mini-CEX) in training the diagnostic thinking ability for clinical interns.Methods 83 interns who came to the First People's Hospital of Lianyungang for clinical practice in June 2015 were divided into the experimental group (45 interns) and the control group (38 interns) according to their schools.The two groups received the same clinical teaching according to internship program of five-year clinical medicine,while the experimental group had to take a weekly Mini-CEX training in chnical sections.The diagnostic thinking ability of the two groups was measured by the Diagnostic Thinking Inventory in Chinese version at the beginning and the end of the internship respectively.SPSS 21.0 was applied to processing the data.Measurement data were expressed as mean plus or minus standard deviation,and t test was used in the comparison between groups.Results At the end of the internship,the total score of diagnostic thinking,the score of knowledge structure in memory and the score of flexibility in thinking of the experimental group were higher than scores at the beginning statistically,and the experimental group had a statistically higher score than the control groups in knowledge structure in memory [(69.36 ± 4.28) vs.(66.95 ± 4.78),P=0.018].Conclusion Mini-CEX training can enhance the clinical diagnostic thinking ability of interns by improving their knowledge structure in memory.

OBJECTIVEThis study aims to investigate the effects of mineral trioxide aggregate (MTA) and calcium hydroxide on proliferation and differentiation of human pulp cells from primary and permanent teeth.

METHODSCell proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. The mRNA expression levels of dentinogenesis-related factors, including alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), and odontoclastogenesis-related factors, such as osteo- protegerin (OPG) and receptor activator of NF-κB ligand (RANKL), were determined by real time polymerase chain reac- tion (PCR).

RESULTSPrimary and permanent pulp cells treated with calcium hydroxide exhibited significantly lower proli- feration rates than the control cells (P<0.01). By contrast, the MTA-treated group showed significantly higher proliferation rates than the control group (P<0.01). Real time PCR results showed that calcium hydroxide-treated primary pulp cells exhi- bited significantly decreased ALP, DSPP, and OPG expression compared with the control group (P<0.01). Conversely, the MTA-treated group displayed significantly increased ALP, DSPP, and OPG expression (P<0.01). Calcium hydroxide-treated primary pulp cells also exhibited significantly upregulated RANKL expression (P < 0.01); by contrast, MTA-treated cells did not show any change in RANKL expression (P>0.05). Likewise, MTA-treated permanent pulp cells showed significantly upregulated ALP and DSPP expression (P < 0.01). However, the calcium hydroxide-treated group remained almost the same as the control group (P > 0.05). Neither MTA nor calcium hydroxide affected OPG and RANKL expression in per- manent pulp cells (P > 0.05).

CONCLUSIONMTA is more suitable as a pulp-capping agent, particularly in primary teeth, than calcium hydroxide.

Aluminum Compounds ; Calcium Compounds ; Calcium Hydroxide ; Cell Differentiation ; Cell Proliferation ; Dental Pulp ; Dentition, Permanent ; Drug Combinations ; Extracellular Matrix Proteins ; Humans ; Oxides ; Phosphoproteins ; Sialoglycoproteins ; Silicates

Adult ; Female ; Humans ; Melanoma ; diagnosis ; pathology ; Neurocutaneous Syndromes ; diagnosis ; pathology

Tumor has long been a hard-nut problem in the world medical field. The effect of the conventional drugs is very limited because of the intervention of multiple micro-environmental factors during the occurrence and progression of tumors. With the characteristics of high efficiency, low toxicity and multi-targets synergistic effect, the long-circulating tumor targeted compound preparations show its unique advantages in improving tumor microenvironment and enhancing the therapeutic effect of treatment, thus it has gradually become a hotspot of studies both at home and abroad. Through consulting a great number of professional literatures at home and abroad in recent years, the authors summarized the current studies in vitro and in vive on long-circulating tumor targeted compound preparations in different carriers, in the expectation of providing new ideas and methods for the development of long-circulating tumor targeted compound preparations.
Animals ; Antineoplastic Agents ; blood ; chemistry ; therapeutic use ; Drug Compounding ; methods ; Humans ; Molecular Targeted Therapy ; methods ; Neoplasms ; blood ; drug therapy

ObjectiveTo investigate the effect of all-trans retinoic acid (atRA) on the renin-angiotensin system (RAS) in 5/6 renal ablation model. MethodsAtRA was administered to 5/6 renal ablation rats by three dosages: 5 mg·kg-1·d-1 (n=8), 10 mg·kg-1d-1 (n=8) and 20 mg·kg-1 d-1 (n=8) and vehicle (vehicle group, n=8) for 10 weeks. Healthy rats consisted of shamoperation group (n =8). The level of renin and angiotensin Ⅱ in renal tissues were measured by radioimmunoassary. The level of angiotensin type 1 receptor (AT1R) in remnant renal cortex was measured by Western blot. The mRNA expression levels of two subunits of activative protein 1(AP-1),c-jun and c-fos was quantitated by real-time PCR. ResultsAfter 10 weeks of atRA treatment by gavarge, artery blood pressure decreased (P＜0.05). AtRA reduced the levels of renin (P＜0.05) and angiotensin Ⅱ (P＜0.05) in kidney and down-regulated the expression of AT1R protein in renal cortex. Larger dose of atRA (20 mg·kg-1·d-1) performed higher activity in inhibiting renin and AT1R. Compared with vehicle group, atRA could significantly inhibit the expression of renal c-jun and c-fos mRNA (P＜0.05). Conclusion atRA can decrease the over-expression of main components of RAS.

Objective To discuss the effect of 5-Aza-CdR on pancreatic carcinoma Capan-2 cell proliferation and PCDH8 gene expression.Methods Pancreatic carcinoma Capan-2 cells were treated with different doses of 5-Aza-CdR with or without gemcitabine,negative control group without drug,0.08μmol/L group with 0.08μmol/L 5-Aza-CdR,0.40 μmol/L group with 0.40 μmol/L 5-Aza-CdR,2.00 μmol/L group with 2.00 μmol/L 5-Aza-CdR,10 μmol/L group with 10 μmol/L 5-Aza-CdR,50 μmol/L group with 50 μmol/L 5-Aza-CdR. The inhibition ratio of Capan-2 cell proliferation were observed by MTT assay and PCDH8 gene and protein expression were detected by RT-PCR and Western blot. Results The inhibition ratio was increased with 5-Aza-CdR dose increasing(P<0.01),but decreased apparently with times extending(P<0.01). Inhibition ratio in 5-Aza-CdR and gemcitabine group was higher than those with only 5-Aza-CdR or gemcitabine groups(P<0.05 or P<0.01).The levels of PCDH8 gene and protein expression were increased significantly in 5-Aza-CdR treatment groups,with dose dependent (P <0.01 ).Conclusion 5-Aza-CdR can inhibit the proliferation of pancreatic carcinoma Capan-2 cell proliferation,and increase PCDH8 gene expression. The inhibition effect is strong when combined with gemcitabine.