Objective Leucine -rich repeat (LRR) -containing G protein -coupled receptor 6 (Lgr6) is glycoprotein hormone receptor and a part of G protein -coupled receptors (GPCRs).Lgr6 is composed of 977 amino acid residues of a polypeptide chain.Having 17 leucine -rich repeats and seven transmembrane domain.In the N -and C -termini are connected with cysteine -rich sequences.It is highest homologous to Lgr4 and Lgr5,but less relationship with thyroid stimulating hormone receptor,progesterone receptor,and follicle -stimulating hormone recep-tor.Research found out that Lgr6 marks in the hair follicles of stem cells can differentiate into all cells in the skin and Lgr6 expression in tumor also has important biological significance.

Text mining provides a new approach for drug repositioning, and the emerging databases and their corresponding tools provide more and more convenience for drug repositioning by text mining.The methods and tools for studying drug repositioning by text mining and their successful application examples were thus described in this paper in order to provide reference for the researchers interested in this field.

Gastroesophageal reflux disease(GERD)is a commonly seen digestive disease. So far,there is no golden standard for the diagnosis of GERD. The main diagnosis methods used in clinical practice include gastroscopy,24 h esophageal pH monitoring and proton pump inhibitor test,however,these methods are either invasive or complex in procedure and with low diagnostic accuracy rate. Gastroesophageal reflux disease questionnaire(GerdQ)possesses multiple advantages such as simple,non-invasive and easily acceptable. This article reviewed the use of GerdQ in diagnosis of GERD.

Systemic lupus erythematosus(SLE) is an autoimmune connective-tissue disorder with a wide range of clinical features.Although the clinical symptoms and immunological findings of pediatric SLE are similar to those of adult SLE patients,the pediatric SLE has some special aspects.In the recent years,although there was no abrupt development in the diagnosis and treatment of SLE,there were some new findings,maybe not mature.On the basis of recent findings outsides,this study drew attention to the advances in the novel clinical manifestation,update treatment of pediatric SLE.

NHL proteins, which play important roles in regulation of cell proliferation and differentiation, have been extensively studied on mammals. Here, we cloned a member of NHL protein family namely BmBrat in silkworm. The full-length cDNA sequence of BmB rat was obtained by means of the rapid amplification of cDNA ends (RACE), including 3 614 bp. The ORF is 2 580 bp long, encoding a protein with 859 amino acid residues. The molecular weight is 94.3 kDa and the isoeledtric point (pI) is 6.65. The BmBrat expression profile was detected by RT-PCR at L5D3 larval stage, and it was expressed in all tissues, including silk gland, midgut, fat body and malpighian tubule. However, it was highly expressed in ovary and head. The expression profile was also detected at different stage of embryo development, and reached a peak at the 4th and 5th days of the embryonic period. Anti-BmBrat polyclonal antibody was generated f6llowing prokaryotic expression, protein purification and mice immunization, which is highly specific and effective for recognizing BmBrat protein through Western blotting and immunofluorescence staining. Subcellular localization of BmBrat in hemocytes revealed that it was specifically expressed in cytoplasm. This study provides a foundation for further research of the biological function of BmBrat gene.
Animals ; Bombyx ; Cloning, Molecular ; DNA, Complementary ; Insect Proteins ; genetics ; metabolism ; Larva ; Mice

Objective We established the animal models of obesity induced by high-fat diet, in order to study the mRNA and protein expression of regulation molecules related with iron metabolism about hepcidin, lipocalin-2 ( LCN2 ) , ferroportin-1 (FPN1) in obese mice’ s liver and the molecular regulation mechanism.Methods C57BL/6J (4 ~6 weeks) mice were randomly divided into control group and obesity model group, each group of ten.The obesity group were fed with a high-fat diet and the control group were given the normal diet for lasting 15 weeks.After we successfully established the obesity animal model, the expression level of hepcidin, LCN2 and FPN1 mRNA in the liver were measured by Real-time fluorescent quantitative PCR method and the protein expression level of LCN2 and FPN1 were measured by Western-Blot.Results Compared with the control group, the expression level of hepcidin mRNA in the liver was increased in obesity group (P <0.05), however, the expression level of LCN2, FPN1 was no significant difference (P >0.05).Conclusion Obesity can increase the expression of hepcidin mRNA, however, there was no significantly effect on the expression of LCN2, FPN1.So, we can’t think that obesity can affect the expression of LCN2 and FPN1, lead to the ability of cells uptake and release iron abnormal, then appear iron metabolism disorders.As a result, leading to iron deficiency.Maybe obesity can affect other regulatory molecules related with iron metabolism through up-regulation the expression of Hepcidin or the more complex regulatory mechanisms.We still need further experimental research and exploration.This research also provides the basis of theoretical and experimental for the further study the effects of obesity on the expression of regulation molecules related with iron metabolism in obesity mice’ s liver and the mechanism of iron deficiency.

Objective To explore the preventive effect of Bailing capsules(BL) on myocardial fibrosis in the viral myocarditis(VMC) mice models and to clarify the possible mechanism.Methods The models of VMC were established by injecting Coxsackie virus B3 (CVB3) solution into 50 BALA/C mice.The 31 mice were divided into model group(n =11),large dose BL group(n =10)and small dose BL group (n =10).At the same time the control group (n =10) was established.The mice in model group and control group were fed with water 1 mL/d,the mice in large dose BL group were fed with BL at a dose of 7.5 g/(kg · d),and the mice in small dose BL group were fed with BL at a dose of 2.5 g/(kg · d).After 60 d,the levels of angiotensin Ⅱ (Ang Ⅱ) in blood serum of mice in various groups were detected by using ELISA.Myocardium tissue of mice was stained by Masson and collagen volume fraction(CVF)was accounted.At the same time,the content of periostin mRNA and transforming growth factor β1 (TGF-[β1) mRNA in myocardium tissue of mice were detected by adopting reverse transcription(RT)-PCR.Results Compared with the control group,the levels of serum Ang Ⅱ,CVF,TGF-β1 mRNA and periostin mRNA were increased (all P ＜ 0.01) in the model group.Compared with the model group,the levels of serum Ang Ⅱ,CVF and TGF-β1 mRNA were obviously decreased in large dose BL group and small dose BL group (all P ＜ 0.05).Compared with the model group,the level of periostin mRNA was obviously decreased in large dose BL group (P ＜ 0.05),but the level of periostin mRNA in small dose BL group was not decreased obviously (P ＞ 0.05).Conclusions BL can offer some protection to myocardial fibrosis in VMC mice.The possible mechanism may be performed through inhibiting the expression of serum Ang Ⅱ and TGF-β1 in myocardium tissue to reduce the expression of periostin.

Objective To assess the reliability and validity of Chinese version National Aeronautics and Space Administration Task Load Index (NASA-TLX) and Subjective Work Assessment Technique (SWAT) in evaluating mental workload of community physicians.Methods One hundred and ten community physicians selected by convenience sampling method from Shanghai were recruited.The split-half reliability,Cronbach's α coefficient and Item-total coefficients were adopted to assess the reliability and the validity of Chinese version NASA-TLX and SWAT scales in evaluating the metal workload of participants.Results For NASA-TLX,the Split-Half,Cronbach's α coefficient was 0.703 and 0.619 respectively,all Item-total coefficients (except for performance item) were greater than 0.4 (P ＜ 0.01).For SWAT,the Split-Half,Cronbach's α coefficient of was 0.647 and 0.669 respectively,Item-total coefficients were all greater than 0.4 (P ＜ 0.01).Factor analysis showed that two scales had good structure validity; item discrimination showed satisfactory item validity (except for performance item).The Spearman correlation coefficients between two scales ranged from 0.359 to 0.465 (P ＜ 0.01).Both of the modified versions of NASA-TLX had better reliability and validity than the original one,the one with removed both performance and frustrate item was better than one with removed performance item only.Conclusions The Chinese version NASA-TLX and SWAT can serve as reliable and valid instruments for assessing the community physicians' perception of mental workload in China; and the modified version of NASA-TLX especially the one with removed performance and frustrate item seems to work better than the original one.

Objective To detect the levels of plasma gelsolin(GSN),vitamin D binding protein(VDBP)and coagulation?related parameters in peripheral blood between pre?eclampsia,then analyze their relationships with the onset or development of pre?eclampsia. Methods A total of 30 women with severe pre?eclampsia(SPE)and 30 women with mild pre?eclampsia(MPE)were enrolled for the study and randomly chosen as trial group. 30 normal pregnancies with matching gestational ages were selected as control group?1 and 30 normal full?term pregnancies were randomly chosen as control group?2. The levels of GSN,VDBP,platelet(PLT),prothrombin time(PT),thrombin time(TT),activated partial thromboplas?tin time(APTT),fibrinogen(FIB),and D?dimer were compared between those groups. Results The levels of GSN,VDBP and PLT,PT,APTT, FIB in SPE group were significantly lower than that of MPE group(P<0.05),and the level of D?dimer was significantly higher(P<0.05). In healthy pregnant women between control group?1 and control group?2,GSN fell during gestational weeks,with a concomitant rise in VDBP with no significance(P>0.05). There were no statistical difference among all the levels of coagulation?related parameters in healthy pregnant women(P>0.05). Conclusion GSN and VDBP levels in pre?eclampsia are significantly lower than that of normal pregnant women,along with pathological hy?percoagulative state,and which are related to severity of disease.

Objective A new type adsorbent for removal of bacterial ET was prepared by immobilizing L-serine covalently onto polyvinylidene difluoride membrane (PVDF-Ser) to study the effect of hemoperfusion on sepsis induced lung injuries in a pig model. Method Endotoxemia were induced in thirteen pigs by injecting endotoxin (1 μg/(kg·h) ) intravenously for 2 h. One hour later, the pigs were treated with absorber of PVDF-Ser volumn (group hemoperfusion) or empty adsorber (group control) for 2 h. Extravascular lung water indexed to body weight and alveolar capillary permeability, levels of serum endotoxin, tumor necrosis factor-α, interleukin-6, malondialdehyde, glutathione, myeloperoxidase activity, and lung histological changes and myeloperoxidase immunohistochemical analysis were measured. Results Hemoperfusion treatment with PVDF-Ser volumn significantly improved extravascular lung water, pulmonary alveolar capillary permeability and lung injuries ( P ＜ 0. 05). In addition, hemoperfusion attenuated both serum and pulmonary cytokines, and decreased lung malondialdehyde and myeloperoxidase levels ( P ＜ 0. 05). Conclusions Hemoperfusion with the adsorber of PVDF-Ser volumn effectively reduced inflammatory responses and improved lung injuries and oxidative stress in endotoxemia. It has a high potential of clinical application for treatment of patients with severe sepsis.