Objective To explore problems of chemical indicative cards and adhesive tapes in the disinfection,and find out some measures for improving the sterilizing effect of the pressure steam sterilizer.Methods 100 stoves of time with various package,dressing and storage tank were disinfected by pulse pressure steam sterilizer and chemical indicative cards of 3M1250 pressure steam sterilizing package at the same pressure,time and temperature.All test wraps were examined and the real conditions were recorded after the sterilization.Results The discoloration of chemical indicative cards in 10 test wraps was not standard before the measures were taken.While after that,it didn't occur again.Conclusion The nonstandard discoloration of chemical indicative cards of 3M1250 pressure steam sterilizer is influenced by condensed water of steam during the steam sterilization.In order to make the chemical indicative cards change into standard black,the accurate using of chemical indicative cards,the standard stuff package and correct using of disinfector are required.

On the basis of the system requirement on medical oxygen supply in modern hospital, from the aspects of rigorous installation, ensuring logical course environment, following related technological standard and enhancing personnel training, the whole quality course management is put up. It is indicate that proper equipment and quality control in evary link are the quarantee of clinical service.

This paper discusses how to strengthen the quality management of low-cost medical consumables in such aspects as purchase,check and acceptance,deposit and provision of low-cost consumables.

Operation rules of sterilizing are put forward.Responsible persons with working licenses are selected.The cleanout,packing,loading of pre-sterilized goods are standardized.Sterilizing effects(physical monitoring,chemical monitoring,biological monitoring,B-D test) are inspected.Requirements in maintenance and metrology test are raised.The content and methods for quality control of sterilized goods by using decontamination device are introduced systematically.It is instructive to enhance decontamination quality and lower infection rate in hospitals.

The general situation of sudden public events is introduced.Considering the characteristics and phases of massive earthquake disaster,the system of emergency medical supplies for sudden public events is set up in terms of storing and managing the emergency medical supplies by reasonable determining the amount of all kinds of emergency medical supplies such as goods reserve,market reserve,production & technology capacity reserve,etc.

Objective To modify the existed transfer trolley for collection and distribution so as to enhance cleaning and disinfection.Methods The existed transfer trolley was added with an intake pipe,a spray nozzle,a drilled divider,a drainage pipe and etc for perfusion rinse,and equipped with auxiliary mechanisms such as a protective support and preserver.Omnidirectional cleaning and disinfection of the trolley were executed by the intake pipe,nozzle and high-pressure irrigation machine.Results The trolley prevented the disinfection solution from spitting,ensured the safety of the staff and environment,and had the qualified rate of disinfection being 100％.Conclusion The modified transfer trolley gains advantages in environmental protection,energy saving and practicability,and thus is worthy promoting clinically.

This study was designed to investigate the potential protective effect of isopimpinelline against para-chlorophenylalanine(PCPA)-induced pineal gland damage in rats.Forty male Sprague-Dawley(SD)rats were divided into four groups(n=10 each):a normal group,a model group,a melatonin-treated group(10 mg/kg),and an isopimpinelline-treated group(1.5 mg/kg).All groups,except for the normal,received intraperitoneal injection of PCPA(450 mg/kg)to induce pineal gland damage.Subsequent treatments were administered orally for 7 days.Sleep latency and duration were evaluated on the sixth day using the pentobarbital sodium sleep synergy test.After the treatment period,serum melatonin levels and pineal gland inflammation markers were assessed alongside oxidative and antioxidative parameters.Histological examinations of the pineal gland were conducted,and the expression of proteins related to the Nrf2 and NF-κB signaling pathways were quantified.Data showed that isopimpinelline alleviates the structural damage in the pineal gland of model rats,significantly elevated serum melatonin levels,and markedly improved sleep latency and duration(P＜0.05).Isopimpinelline activated the Nrf2 signaling pathway by inhibiting Keap1 expression,which facilitated the nuclear translocation of Nrf2 and upregulated the antioxidant proteins NQO1 and HO-1,thereby mitigating oxidative stress in the pineal gland(P＜0.05).Furthermore,isopimpinelline significantly reduced the levels of pro-inflammatory cytokines IL-2,TNF-α and IL-6.Isopimpinelline also suppressed the NF-κB signaling pathway,reducing the expression of NF-κB p65,IKKβ,and p-IKKβ proteins,as well as the nuclear translocation of NF-κB p65(P＜0.05),thereby providing anti-inflammatory benefits.In conclusion,isopimpinelline could protect pineal gland from damage by activating the Nrf2 signaling pathway and inhibiting the NF-κB pathway.

As a representative chemotherapeutic drug, docetaxel (DTX) has been used for breast cancer treatment for decades. However, the poor solubility of DTX limits its efficacy, and the DTX based therapy increases the metastasis risk due to the upregulation of C-X-C chemokine receptor type 4 (CXCR4) expression during the treatment. Herein, we conjugated CXCR4 antagonist peptide (CTCE) with DTX (termed CTCE-DTX) as an anti-metastasis agent to treat breast cancer. CTCE-DTX could self-assemble to nanoparticles, targeting CXCR4-upregulated metastatic tumor cells and enhancing the DTX efficacy. Thus, the CTCE-DTX NPs achieved promising efficacy on inhibiting both bone-specific metastasis and lung metastasis of triple-negative breast cancer. Our work provided a rational strategy on designing peptide-drug conjugates with synergistic anti-tumor efficacy.