This study was conducted to determine the prevalence of bacterial vaginosis among women of age 20 to 59 years at Sarawak General Hospital by examining the conventional Pap smear. The prevalence of this infection was studied among the five main ethnic groups comprising the Iban, Chinese, Malay, Bidayuh and Orang Ulu. Microscopic screening of 300 conventional Pap smears which had been processed and stained with the Papanicolaou staining method was conducted. Based on the information provided in the Pap smear screening form, the clinical manifestations were observed which may provide some indications on the type of infection. The details of the clinical manifestations were very important because in an infection or inflammation, Pap smears would usually demonstrate changes on the cyto-morphology of the epithelial cells. The age factor among the different ethnics also played important role in the assessment of the infection frequency. Bacterial vaginosis was detected in 79.7% (239/300) of cases. The infection occurred in all ethnic groups and the infections were more frequently seen among the 20 to 39 years age group. Majority of the cases (93.9%) presented with clinical signs and symptoms. The results of this study revealed the importance of undergoing a Pap smear screening for bacterial vaginosis detection besides screening for the early detection of cervical cancer

Determination of races in the analysis of hair on scalp is one of the challenges faced in forensic analysis to identify victims, suspects and crime scene. This study aimed to determine the differences among the two main races in Malaysia which included Malay and Chinese, through the examination of the characteristics of hair. These includes general features of the hair, patterns of cuticle, colors, cortex pigment distribution and characteristics of medulla - types, thickness and medulla index. This study was carried out by using a light microscope and consisted of five steps, which were sample preparation, examination of common features of hair, preparation and examination of cuticle scales matrix, cortex and medulla. Result showed that most of the Malay have dark brown pigments while Chinese have pigments of light brown color. We also noted that thickness of medulla can also be used to differentiate between Malay and Chinese where average values for Malay and Chinese are 7.59 ± 1.45 μm and 9.12 ± 1.85 μm, respectively. The average values of medulla index for Malay and Chinese were noticeably different at 0.17 ± 0.02 μm and 0.18 ± 0.02 μm, respectively. However, textures, thickness of hair, patterns of cuticle scales, and types of medulla do not show any significant difference between Malay and Chinese. In conclusion, this study has shown that pigment colors, thickness of medulla and medulla index are important characteristics that can be used to determine race

Ficus deltoidea leaves were widely used as a tea beverages in Malaysia with no information of its mineral content. Hence the mineral content of two species of Ficus deltoidea leaves were investigated. The dried leaves of F. deltoidea var. angustifolia and F. deltoidea var. deltoidea were acid digested and mineral elements of Na, Mg, K, Ca, Mn, Cr, Fe and Zn were determined using ICP–MS. Magnesium, potassium, sodium, manganese, iron and zinc were found to be present in the leaves of F. deltoidea var. angustifolia and F. deltoidea var. deltoidea. Concentration of magnesium (1934 mg/L), manganese (58.37 mg/L), iron (6.89 mg/L) and zinc (1.77 mg/L) in F. deltoidea var. deltoidea species were significantly (P < 0.05) higher than in F. deltoidea var. angustifolia species with concentration of 317 mg/L, 29.62 mg/L, 4.55 mg/L and 1.26 mg/L for magnesium, manganese, iron and zinc respectively. Meanwhile, concentration of sodium in F. deltoidea var. deltoidea species (3.13 mg/L) was found to be significantly (P < 0.05) lower than the concentration in F. deltoidea var. angustifolia species (9.11 mg/L). The finding showed that the leaves of F. deltoidea var. deltoidea has higher nutritional value than the leaves of F. deltoidea var. angustifolia. Leaves of Ficus deltoidea especially the F. deltoidea var. deltoidea species contain high amount of magnesium, manganese and potassium. Therefore, tea made of this leaves can be served as a good source of minerals for human consumption

Pattern electroretinogram (pERG) is a recording of retinal response to a phase reversing pattern stimulus. It gives information on the integrity of the inner retina specifically the ganglion cells. The recording of pERG in a laboratory is easily influenced by many factors hence standardization in a laboratory is required to ensure that pERG recorded is repeatable and reproducible. The objective of this study was to determine the factors that could affect pERG recordings at the Electrophysiology Laboratory, Optometry Program, Faculty of Health Sciences (FSK), Universiti Kebangsaan Malaysia (UKM). This study involved forty-five subjects, aged between 20 and 25 years. They were divided into three groups. The factors being investigated were effects of 1) Alcaine 0.5%, 2) diurnal variation and 3) different size and shape of fixation target for pERG measurement (amplitude and implicit time). Paired t-test showed no significant differences between the recording before and after instillation of Alcaine 0.5% for amplitude (p = 0.116) and implicit time (p = 0.557). Measurements carried out in the morning and afternoon did not show significant differences for amplitude (p = 0.864) and implicit time (p = 0.174). For different fixation targets, results also showed no significant difference for amplitude (p = 0.125) and implicit time (p = 0.404). In conclusion, use of Alcaine 0.5%, different time of measurements and different type of fixation targets do not influence pERG results at the Electrophysiology Laboratory, FSK, UKM. Results of this study can be used for comparisons in research or in diagnosing diseases of the retina in the future

Thyroid and gonads are radiosensitive organs which requires radiation shield to reduce the dose received. However, radiation shielding is not widely used in radionuclide imaging because it is heavy, uncomfortable and can cause pain in the spine. Therefore, a research was carried out to determine the ability of thyroid and gonad radiation shield which is thinner and lighter in reducing radiation dose. A study was conducted in Hospital Putrajaya to determine the radiation dose received by the thyroid and gonads during a complete Positron Emission Tomography-Computed Tomography (PET-CT) procedure with and without radiation shield. A total of six male staffs have been chosen as subject and data from 33 complete PET-CT procedures have been collected. For every PET-CT procedure, the subject’s thyroid and gonad were shielded using 0.5-mm thick radiation shielded, model Mavig 615 (USA) and Shielding International (USA) respectively. Thermal luminescent dosimeter (TLD) chips were used as radiation dose detector. The average 18FFDG radioactivity administered to the patient was 387 MBq and the average scan time is 9.224 ± 1.797 minutes. The results showed that the mean equivalent dose received by the thyroid with and without shielding were 0.080 ± 0.033 mSv and 0.078 ± 0.039 mSv respectively. The mean equivalent dose received by gonad with and without shielding were 0.059 ± 0.040 mSv and 0.061 ± 0.030 mSv respectively. Radiation shield with 0.5 mm thickness is unable to reduce radiation dose received by the thyroid (p = 0.76) and gonads (p = 0.79) because it is too thin to resist the high-energy radiation during PET-CT procedures. Thyroid receive higher radiation dose of 0.016 m Sv compared to the gonads (p < 0.05) because the thyroid’s position is more exposed to radiation sources which are 18F-FDG during radiopharmaceutical preparation and patients after administered with 18F-FDG during PET-CT procedure.

This study aimed to identify the foot sensory status of Malay women with type 2 diabetes mellitus and to determine its relation with: demographic factors, glycated haemoglobin level in plasma, and body mass index. A cross-sectional study was conducted on 71 Malay women with type 2 diabetes mellitus who attend three health clinics in Hulu Langat District, Selangor. Foot sensation was assessed using the Semmes-Weinstein Monofilament 5.07 in nine locations on the plantar and dorsal of the feet. Loss of protective sensation was defined as inability to sense the monofilament in one or more sites of either foot. This study found that 56.3% of women had loss of protective sensation, with common sites being the heel, fifth metatarsal head, lateral mid-foot and little toe. Duration of diabetes mellitus were significantly related to foot sensation. The age, HbA1C level and body mass index did not show any significant correlation. Loss of protective sensation may lead to serious foot complication and therefore early screening involving multidisciplinary team is essential for prevention

In forensic entomology practice, it is more common to use raw animal tissue to breed dipteran larvae and it often brings unpleasant odour in the laboratory. Few studies suggested the use of synthetic diets, mainly agar-based media, as alternatives to animal tissue but it is rarely being practiced in forensic entomology laboratory. The present study observed the growth of a forensically important fly, Megaselia scalaris (Loew) on raw cow’s liver, nutrient agar, casein agar and cow’s liver agar. A total of 100 M. scalaris eggs were transferred each into the different media and placed in an incubator at 30°C in a continuous dark condition. Data on length and developmental period were collected by randomly sampling three of the largest larvae from each rearing media, twice a day at 0900 and 1500 hours until pupariation. M. scalaris larvae reared on raw cow’s liver recorded the highest mean length (4.23 ± 1.96 mm) followed by cow’s liver agar (3.79 ± 1.62 mm), casein agar (3.14 ± 1.16 mm) and nutrient agar (3.09 ± 1.11 mm). Larval length in raw liver and liver agar were significantly different from those in nutrient and casein agar (p < 0.05). Larvae bred in liver agar and raw liver recorded the shortest larval duration before entering the post-feeding stage (89 hours), followed by nutrient agar (119 hours) and casein agar (184 hours). Total developmental time from oviposition until adult emergence for M. scalaris in liver agar and raw liver was approximately 163 hours. All puparia in nutrient agar and casein agar failed to hatch. This research highlighted the potential use of cow’s liver agar as an alternative diet of raw liver to culture M. scalaris in laboratory.

A study on the isolation and identification of bacterial flora from hot spring was carried out using water from Bentong hot spring in Pahang. In general most of the bacteria identified from the hot springs were from Bacillus sp. This was due to its ability to adapt to high temperature mainly by forming endospore. The objective of this study was to identify bacteria other than Bacillus that were able to survive in hot springs. Three hot spring pools were identified (A,B and C) with temperatures ranging from 36°C to 52°C and pH values between 7.88-8.65. This study has succesfully isolated two species of Gram positive cocci and three species of Gram negative bacilli. The two Gram positives were Streptococcus sp. and Staphylococcus sp. The three species of Gram negative rods were unidentified and were denoted as X, XI and XII.

This case study was conducted to determine the level of personal hygiene among food handlers and its relationship to cafeteria hygiene Universiti Kebangsaan Malaysia (UKM). 75 respondents were involved in this study. The data shows that 32% of respondents are not vaccinated for typhoid while 49.3% do not acquire food handlers’ course certificate. The data also shows that 50.7% of the respondents are not Malaysian citizen. In term of personal hygiene practices, the findings show that 7 items are higher in percentage (70.3% – 87.5%) while another 7 items are lower in percentage (26.4% – 68%). The findings also show that 12 items related to cafeteria sanitary are conformed while 9 items are not. The statistical analysis shows that personal hygiene practices are significantly different (U = 472, p = 0.01) between Malaysian citizen food handlers and the non-Malaysian food handlers. The findings show that Malaysian citizen food-handlers practice better personal hygiene in comparison to non-Malaysian food-handlers. It is also observed that the relationship between is positive and statistically significant (χ2 = 6.432, p = 0.011), although the association between food-handlers personal hygiene and cafeteria sanitary is medium (Φ = 0.29). The findings imply that Malaysian citizen food handlers are better in personal hygiene practices and in promoting cafeteria sanitary in comparison to non-Malaysian food-handlers

Pesticide exposure can lead to low trace elements levels in human body. Trace element plays important role in body metabolism. The aim of this study was to study the levels of selenium, zinc and chromium among paddy farmers who expose to pesticide in Wilayah I, MADA, Perlis. This cross sectional study involved 70 males paddy farmers and 57subjects living in fisherman village as control group who were aged between 21 to 80 years old. Subjects were interviewed to obtain information on their demographic data by using validated questionnaire. Subjects also were examined for their blood pressure and glucose level. Selenium, zinc and chromium levels were analyzed by using acid digestion method and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Results showed that selenium levels in hairs (5.11 ± 17.05 μg/L) and nails (4.92 ± 2.17 μg/L) were significantly (p < 0.05) lower compared to selenium levels in hairs (15.67 ± 10.59 μg/L) and nails (6.67 ± 2.81 μg/L) in control group. Chromium levels in hairs (31.83 ± 15.17 μg/L) and nails (87.64 ± 23.30 μg/L) were also significantly lower (p < 0.05) compared to chromium levels in hairs (85.19 ± 56.90 μg/L) and nails (99.36 ± 56.89 μg/L) of control group. However there were no significant different (p>0.05) between all trace element levels and duration of pesticide exposures. In conclusion, levels of trace elements were lower in nails and hairs of paddy farmers than fisherman community group