1.Evaluation of Rapid Diagnostics for Plasmodium falciparum and P. vivax in Mae Sot Malaria Endemic Area, Thailand.
Wanna CHAIJAROENKUL ; Thanee WONGCHAI ; Ronnatrai RUANGWEERAYUT ; Kesara NA-BANGCHANG
The Korean Journal of Parasitology 2011;49(1):33-38
Prompt and accurate diagnosis of malaria is the key to prevent disease morbidity and mortality. This study was carried out to evaluate diagnostic performance of 3 commercial rapid detection tests (RDTs), i.e., Malaria Antigen Pf/Pantrade mark, Malaria Ag-Pftrade mark, and Malaria Ag-Pvtrade mark tests, in comparison with the microscopic and PCR methods. A total of 460 blood samples microscopically positive for Plasmodium falciparum (211 samples), P. vivax (218), mixed with P. falciparum and P. vivax (30), or P. ovale (1), and 124 samples of healthy subjects or patients with other fever-related infections, were collected. The sensitivities of Malaria Ag-Pftrade mark and Malaria Antigen Pf/Pantrade mark compared with the microscopic method for P. falciparum or P. vivax detection were 97.6% and 99.0%, or 98.6% and 99.0%, respectively. The specificities of Malaria Ag-Pftrade mark, Malaria Ag-Pvtrade mark, and Malaria Antigen Pf/Pantrade mark were 93.3%, 98.8%, and 94.4%, respectively. The sensitivities of Malaria Ag-Pftrade mark, Malaria Antigen Pf/Pantrade mark, and microscopic method, when PCR was used as a reference method for P. falciparum or P. vivax detection were 91.8%, 100%, and 96.7%, or 91.9%, 92.6%, and 97.3%, respectively. The specificities of Malaria Ag-Pftrade mark, Malaria Ag-Pvtrade mark, Malaria Antigen Pf/Pantrade mark, and microscopic method were 66.2%, 92.7%, 73.9%, and 78.2%, respectively. Results indicated that the diagnostic performances of all the commercial RDTs are satisfactory for application to malaria diagnosis.
Antigens, Protozoan/blood
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Cross-Sectional Studies
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*Diagnostic Techniques and Procedures/instrumentation
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Endemic Diseases/statistics & numerical data
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Humans
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Malaria/*diagnosis/epidemiology/parasitology
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Malaria, Vivax
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Plasmodium falciparum/genetics/immunology/*isolation & purification
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Reagent Kits, Diagnostic
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Thailand/epidemiology
2.Nested-PCR and a New ELISA-Based NovaLisa Test Kit for Malaria Diagnosis in an Endemic Area of Thailand.
Pimwan THONGDEE ; Wanna CHAIJAROENKUL ; Jiraporn KUESAP ; Kesara NA-BANGCHANG
The Korean Journal of Parasitology 2014;52(4):377-381
Microscopy is considered as the gold standard for malaria diagnosis although its wide application is limited by the requirement of highly experienced microscopists. PCR and serological tests provide efficient diagnostic performance and have been applied for malaria diagnosis and research. The aim of this study was to investigate the diagnostic performance of nested PCR and a recently developed an ELISA-based new rapid diagnosis test (RDT), NovaLisa test kit, for diagnosis of malaria infection, using microscopic method as the gold standard. The performance of nested-PCR as a malaria diagnostic tool is excellent with respect to its high accuracy, sensitivity, specificity, and ability to discriminate Plasmodium species. The sensitivity and specificity of nested-PCR compared with the microscopic method for detection of Plasmodium falciparum, Plasmodium vivax, and P. falciparum/P. vivax mixed infection were 71.4 vs 100%, 100 vs 98.7%, and 100 vs 95.0%, respectively. The sensitivity and specificity of the ELISA-based NovaLisa test kit compared with the microscopic method for detection of Plasmodium genus were 89.0 vs 91.6%, respectively. NovaLisa test kit provided comparable diagnostic performance. Its relatively low cost, simplicity, and rapidity enables large scale field application.
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Coinfection/*diagnosis/epidemiology
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Endemic Diseases
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Enzyme-Linked Immunosorbent Assay/methods
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Female
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Humans
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Malaria, Falciparum/*diagnosis/epidemiology
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Malaria, Vivax/*diagnosis/epidemiology
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Male
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Middle Aged
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Molecular Diagnostic Techniques/*methods
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Polymerase Chain Reaction/*methods
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Sensitivity and Specificity
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Serologic Tests/methods
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Thailand/epidemiology
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Young Adult
3.Evaluation of the Accuracy of the EasyTest(TM) Malaria Pf/Pan Ag, a Rapid Diagnostic Test, in Uganda.
Chom Kyu CHONG ; Pyo Yun CHO ; Byoung Kuk NA ; Seong Kyu AHN ; Jin Su KIM ; Jin Soo LEE ; Sung Keun LEE ; Eun Taek HAN ; Hak Yong KIM ; Yun Kyu PARK ; Seok Ho CHA ; Tong Soo KIM
The Korean Journal of Parasitology 2014;52(5):501-505
In recent years, rapid diagnostic tests (RDTs) have been widely used for malaria detection, primarily because of their simple operation, fast results, and straightforward interpretation. The Asan EasyTest(TM) Malaria Pf/Pan Ag is one of the most commonly used malaria RDTs in several countries, including Korea and India. In this study, we tested the diagnostic performance of this RDT in Uganda to evaluate its usefulness for field diagnosis of malaria in this country. Microscopic and PCR analyses, and the Asan EasyTest(TM) Malaria Pf/Pan Ag rapid diagnostic test, were performed on blood samples from 185 individuals with suspected malaria in several villages in Uganda. Compared to the microscopic analysis, the sensitivity of the RDT to detect malaria infection was 95.8% and 83.3% for Plasmodium falciparum and non-P. falciparum, respectively. Although the diagnostic sensitivity of the RDT decreased when parasitemia was < or =500 parasites/microl, it showed 96.8% sensitivity (98.4% for P. falciparum and 93.8% for non-P. falciparum) in blood samples with parasitemia > or =100 parasites/microl. The specificity of the RDT was 97.3% for P. falciparum and 97.3% for non-P. falciparum. These results collectively suggest that the accuracy of the Asan EasyTest(TM) Malaria Pf/Pan Ag makes it an effective point-of-care diagnostic tool for malaria in Uganda.
Adolescent
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Adult
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Antigens, Protozoan/blood/*isolation & purification
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Child
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Child, Preschool
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Humans
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Malaria, Falciparum/*diagnosis/epidemiology
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Parasitemia
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Point-of-Care Systems
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Predictive Value of Tests
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Reagent Kits, Diagnostic
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Sensitivity and Specificity
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Uganda/epidemiology
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Young Adult
4.An Alternative Method for Extracting Plasmodium DNA from EDTA Whole Blood for Malaria Diagnosis
Krongkaew SEESUI ; Kanokwan IMTAWIL ; Phimphakon CHANETMAHUN ; Porntip LAUMMAUNWAI ; Thidarut BOONMARS
The Korean Journal of Parasitology 2018;56(1):25-32
Molecular techniques have been introduced for malaria diagnosis because they offer greater sensitivity and specificity than microscopic examinations. Therefore, DNA isolation methods have been developed for easy preparation and cost effectiveness. The present study described a simple protocol for Plasmodium DNA isolation from EDTA-whole blood. This study demonstrated that after heating infected blood samples with Tris–EDTA buffer and proteinase K solution, without isolation and purification steps, the supernatant can be used as a DNA template for amplification by PCR. The sensitivity of the extracted DNA of Plasmodium falciparum and Plasmodium vivax was separately analyzed by both PCR and semi-nested PCR (Sn-PCR). The results revealed that for PCR the limit of detection was 40 parasites/μl for P. falciparum and 35.2 parasites/μl for P. vivax, whereas for Sn-PCR the limit of detection was 1.6 parasites/μl for P. falciparum and 1.4 parasites/μl for P. vivax. This new method was then verified by DNA extraction of whole blood from 11 asymptomatic Myanmar migrant workers and analyzed by Sn-PCR. The results revealed that DNA can be extracted from all samples, and there were 2 positive samples for Plasmodium (P. falciparum and P. vivax). Therefore, the protocol can be an alternative method for DNA extraction in laboratories with limited resources and a lack of trained technicians for malaria diagnosis. In addition, this protocol can be applied for subclinical cases, and this will be helpful for epidemiology and control.
Cost-Benefit Analysis
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Diagnosis
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DNA
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Edetic Acid
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Endopeptidase K
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Epidemiology
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Heating
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Hot Temperature
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Humans
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Limit of Detection
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Malaria
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Methods
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Myanmar
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Plasmodium falciparum
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Plasmodium vivax
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Plasmodium
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Polymerase Chain Reaction
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Sensitivity and Specificity
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Transients and Migrants