Objective To investigate the risk factors and the immune functions for noeocomial palmonary fungal infection (PFI) in COPD patients.Methods The data of 55 cases of nosocomial PFI were analyzed,and examined the level of T Lymphocyte Subsets (TLS) in peripheral blood from 67 cases COPD,then compared with 54 healthy donors.Results Nosocomial pulmonary fungal infection Candida Albicans was ranked the first pathogen.CD3 and CD4 of COPD with PFI were significantly ower than COPD and healthy donors(P＜0.05).Conclusions We should improve the immunity of patients in the process of therapy.We should reasonable use of bread spectrum antibiotics and corticosteroids in order to reduce incidence of pulmonary fungal infection.

Objective To evaluate MR appearances of normal and injured anterior cruciate ligament(ACL).Methods The MR findings of normal ACL in 16 cases and ACL tear in 15 cases were reviewed retrospectively.Results Normal ACL appeared as a flat band with low signal intensity on T1 and T2-weighted imaging. The injured ACL can appeared as interruption in continuation(n=5), irregular shape(n=1),abnormal direction(n=2) and increased in signal intensity(n=7).Conclusion MRI is a accurate and noninvasive diagnostic method for ACL tear.

Fibroblasts ; Heart ; Humans

AIM:To check the physical interaction between GST-Na+-K+-ATPase domain and recombinant human augmenter of liver regeneration (rhALR) by GST pull down assay. METHODS: With PCR and genetic recombinant techniques, the coding region of ? subunit of Na+-K+-ATPase was cloned into expressing plasmid pGEX-4T and identified by endonuclease digestion and sequencing methods. Under the inducing of 0.1 mmol/L IPTG, the fusion protein GST-Na+-K+-ATPase domain was highly expressed by E.coli DH-5?. After hypersound quassating, the GST-Na+-K+-ATPase domain was purified by glutathione agarose beads and the physical interaction with rhALR was checked by GST pull down assay. RESULTS: Analysis by SDS-PAGE showed the rhALRs of monomer and dimmer in GST-Na+-K+-ATPase domain lane. The Western blotting of the GST-pull down assay showed the same results as well. CONCLUSION: The Na+-K+-ATPase domain is associated with rhALR specifically in vitro.

Objective To analyze the tendency and influential factors and relationship for behavior problem among 3 to 6 years old preschool children.Methods By a prospective study,the questionnaire survey was carried out in 551 parents of 3 to 6 years old children from kindergarten in Guangzhou,including General Situation Questionnaire,the Strengths and Difficulties Questionnaire(parent version) and 3-7 years old children Behavioral Style Questionnaire.The data were collected at baseline and 1 year follow-up,and were analyzed by the prospective study method.Results Baseline survey 733 valid questionnaires,551 questionnaires were followed up (follow-up rate was 75.2％),the effective questionnaires were 526 (effective rate was 95.5％).The detection rate of behavior problem reduced by age (t =1.975,P =0.049),especially in the kind problem of conduct (t =2.888,P =0.004),friendship (t =2.572,P =0.010) and prosocial behavior(t =-3.329,P =0.001).In the mean time,the degree of cutting down in girls was more obvious than in boys (t =2.359,P =0.019).The influential factors include mother'age,parental education level,structure of family and children' temperament.Children's gender and whether the only child has no significant effect on behavior problems,and the family income only affects the change of behavior problems.Conclusions The detection rate of behavior problem reduce by age and influenced by many factors.Intervention measures must be synthetically considered various related factors.

This paper analyzed the moral construction in the prenatal diagnosis of surgical nursing and nursing features, including:strictness and complexity , professional and comprehensive , collaborative, and independence , carry out from the patients with preoperative evaluation , operation and postoperative effect evaluation every link and detail, with the theory of nursing ethics to guide and standardize nursing , dealing with the relation between various ethic, improve patient satisfaction , based on the premise of mutual modern relationship between nurses and pa-tients.

Objective To apply fluorescence-activated cell sorting (FACS) and magnetic activated cell sorting (MACS) in sorting natural killer (NK) cells from human peripheral blood. The efficiency of these two methods were compared so that the basic conditions for the further study on the lower proportion of peripheral blood cells could be created. Methods Twenty cases of normal peripheral blood and 3 cases of post-hematopoietic stem cell transplantation (post-HSCT) were collected and NK cell were sorted by FACS and MACS. Results Before sorting the normal peripheral blood, the proportion of the NK cell in the leukocyte was (12.86±3.62) %, the purity of NK cell was up to (96.15±2.03) % and the recovery was (95.08±2.16) %after sorting by FACS, the purity was up to (93.35±3.61) % and the recovery was (94.11±3.01) % by MACS.And before sorting the post-HSCT peripheral blood, the proportion of the NK cell in the leukocyte was (11.01±2.08) %, the purity of NK cell was up to (96.22±2.16) % and the recovery was (95.27±1.18) % after sorting by FACS. The purity was up to (90.98±1.94) % and the recovery was (94.54±3.52) % by MACS. Thereis difference in purity of NK cell (t = 5.925, P ＜0.05), while no difference in rate of recovery between 2methods (t = 0.789, P ＞0.05). Conclusion FACS is more efficient, rapidly and easily than MACS in sorting NK cell, especially for post-HSCT cases.

To evaluate the association of ealpain-10 gene UCSNP-43 polyorphism with type 2 diabetes in Chinese Han population.Meta-analysis showed that calpain-10 gene UCSNP-43 polymorphism may be associated with type 2 diabetes in Chinese Han population.Allele G and genotypo GG may be risk factors for type 2 diabetes,while allele A and genotype GA may be the protective factors.

Objective To investigate the biological activities of a conditioned medium for human dermal papilla. Methods Culture medium of the lower passage human dermal papilla cells was collected as the conditioned medium. The growth pattern and the growth curve of the higher passage human dermal papilla cells cultured with conditioned medium were observed in vitro. And the morphology of the co-culture of the higher passage human dermal papilla cells and the lower passage human dermal papilla cells was observed. Results The higher passage human dermal papilla cells, which was cultured with conditioned medium from the lower passage human dermal papilla cells, showed aggregative growth pattern. And the growth curve of the higher passage human dermal papilla cells was much better than that in the control groups (P

Objective To evaluate the value of clinical data and various methods of diagnostic imaging in the patients with pancreatic cancer. Methods Two hundreds and fifty-six patients with pancreatic cancer admitted into Peking Union Medical College Hospital between 1996 and 2004 were included. The clinical symptom and the accuracy of various imaging methods (B-US, CT, ERCP, EUS, DSA and MRI) in assessing pancreatic carcinoma were analyzed. Results Weight lost was the most common symptom in 79% of patients with pancreatic carcinoma. Jaundice was mostly seen in patients with pancreatic head carcinoma. Upper abdominal pain was in 86% of patients with carcinoma in pancreatic body and tail. The diagnostic accuracies of B-US, CT, ERCP, EUS, DSA and MRI were 83% , 89% , 90% , 91% , 82% and 79% respectively. Conclusion B-US and CT scanning should be undergone in patients with abdominal pain, jaundice and weight lost who were suspected to have pancreatic carcinoma. Further imaging techniques such as EUS, ERCP and MRI should be advised in patients highly suspected to have pancreatic carcinoma, in spite of the results of B-US and CT were negative.