Objectives: This paper presents a reference data model for blood bank management to control blood inventories considering real-world uncertainties and constraints. It helps information systems identify blood product status for various critical decisions (such as replenishment, assignment, and issuing) instantly. Additionally, some significant optimization concepts of the inventory management literature for blood wastage and shortage reduction, such as clearance sale and substitution based on medical priorities, are applied in the model. Methods: The proposed model was constructed by object-oriented and ICAM (Integrated Computer Aided Manufacturing) definition ɸ (IDEF0) techniques for function modeling. Through semi-structured questionnaires and interviews, the research team elicited and classified user requirements. Then, the demand-centered sub-processes and comprehensive functions were mapped to manage the process. Results: The model captures and integrates the top-level features of the inventory system entities. It also provides insights into a developed data dictionary to understand the system’s elements and attributes, where a data item fits in the structure, and what values it may contain. For designing the system’s process and following-up data, the main relevant inputs are considered. Conclusions A flexible and applicable demand-centered framework for managing a typical blood bank’s inventory process was developed by focusing on user requirements. The proposed model can be applied to design and monitor inventory information and decision-support systems. The model provides real-time iterative dynamic process insights. It can also provide the data needed for logistic planning systems and the design of blood operational infrastructure.

INTRODUCTIONThe aim of this study was to determine the effect of a high-fat diet (HFD) on oocyte maturation and quality in a mouse model.

METHODSFemale BALB/c mice were allocated to one of the following groups: (a) control group (n = 40), which received a controlled diet; or (b) HFD group (n = 40), which received an HFD for 12 weeks. Sections of the ovary were examined histologically. The number of follicles and corpora lutea were counted. In vitro maturation and in vitro fertilisation (IVF) were assessed in germinal vesicle (GV) and metaphase II (MII) oocytes, respectively. The expression of bone morphogenetic protein 15 (BMP15) and leptin receptor genes in GV and MII oocytes was evaluated using reverse transcription real-time polymerase chain reactions.

RESULTSIn the HFD group, there was a decreased number of primordial and Graafian follicles, as well as corpora lutea (p < 0.05). The rate of oocyte development to the MII stage was also reduced (p < 0.001). Cumulus expansion was observed more frequently in the control group than the HFD group (p < 0.05). The IVF rate in the HFD group was lower than that in the control group (p < 0.05). In the HFD group, BMP15 and leptin receptor genes were upregulated in the GV stage (p > 0.05) and MII stage (p < 0.05), compared to the control group.

CONCLUSIONAn HFD reduces folliculogenesis in the primordial and Graafian stages, in vitro maturation and in vitro fertilisation rates, as well as oocyte quality in mice.

Animals ; Body Weight ; Bone Morphogenetic Protein 15 ; metabolism ; Corpus Luteum ; pathology ; Diet, High-Fat ; Female ; Fertility ; Fertilization in Vitro ; methods ; Gene Expression Regulation ; Metaphase ; Mice ; Mice, Inbred BALB C ; Obesity ; complications ; Oocytes ; cytology ; pathology ; Ovarian Follicle ; pathology ; Ovary ; metabolism ; pathology ; Photography ; Polymerase Chain Reaction ; Receptors, Leptin ; metabolism