OBJECTIVETo reveal the factors that affect the contents of volatile oil and beta-elemene in oil in Sarcandrae and to provide scientific basis for Sarcandrae's reasonable exploitation and quality assessment.

METHODA GC method was established and the contents of volatile oil and beta-elemene were determined in Sarcandrae from different length of time to grow, different medicament portions, different collection periods and different length of time to dry.

RESULTThe length of time to grow had no significant effect and the collection periods had effect on the volatile oil yield and content of beta-elemene. The volatile oil yield and content of beta-elemene were the highest in Sarcandrae harvested in December. The yield of volatile oil in different medicament portions descended in an order of roots, leaves and stems. The content of beta-elemene was the highest in leaves and the lowest in stems. With the increasing of the length of time to dry, the volatile oil yield and content of beta-elemene decreased calculated on an anhydrous basis.

CONCLUSIONThe established method is simple, accurate and repeatable. It can be used for the quality control of beta-elemene in Sarcandrae. The study provides a valuable basis for the quality assessment, the development and utilization of Sarcandrae.

Magnoliopsida ; chemistry ; Oils, Volatile ; chemistry ; Plant Leaves ; chemistry ; Sesquiterpenes ; chemistry

OBJECTIVETo provide evidences for the pharmacognostical and chemical identification and the further development of Lysinotus wilsonii.

METHODThe paraffin section method was used for the microscopic identifications of stems and leaves. The slide with chloral hydrate was applied for the microscopic identifications of the powder of stems and leaves. The HPLC was used for the identification of the phenylpropanoids.

RESULTAn obvious periderm consisted of a line of inseparable cells, lots of stone cells existed in the periderm and cortex, narrow ring of xylem and broad pith could be easily observed in the stem transaction of L. wilsonii. Epidermis was composed of one lines of parenchyma, three lines of epithelial cells in the side of above epidermis, palisade tissue was composed of 2-3 lines of square and thin cells and amphicribral vascular bundle in transaction of midrib also could be observed in the leaf transaction of L. wilsonii. Long xylem fibers, lots of pitted vessels, stone cells and anomocytic type stomas existed in the powder of L. wilsonii. Acteosidel and caleolarioside B were detected in L wilsonii.

CONCLUSIONThe pharmacognostic and chemical characteristics of L. wilsonii can be used for authentication of the plant.

Magnoliopsida ; anatomy & histology ; chemistry ; Plant Extracts ; analysis

OBJECTIVETo study the alkaloids of the Chinese medicinal herbs Daphniphyllum macropodum for in search for new bioactive substances.

METHODThe CHCl3 extract of D. macropodum was subjected to repeated column chromatography on silica gel to afford four pure compounds (1-4). Their structures were determined on the basis of detailed spectroscopic analysis and comparison with the data reported in the literature.

RESULTThe four known compounds were isolated and elucidated as Daphniphyllum alkaloids with different carbon skeletons, namely longistylumphylline A (1), deoxycalyciphylline B (2), daphnicyclidin B (3) and H (4), respectively.

CONCLUSIONAll compounds were isolated from the titled plant for the first time. The discovery of daphnicyclidin B (3) and H (4) further confirmed the biogenetic relationship between the two compounds and the previously reported macropodumines A-C, found in the same plant.

Alkaloids ; chemistry ; isolation & purification ; Drugs, Chinese Herbal ; analysis ; Magnoliopsida ; chemistry

OBJECTIVETo study the chemical constituents from Aeschynanthus longicaullis.

METHODThe column chromatographic techniques were applied to isolate the constituents. The spectroscopie methods were used of EI-MS and NMR to identify the structures of the separated compounds.

RESULTSeven compounds were isolated from the ethyl acetate extract of A. longicaullis, whose structures were elucidated as cryptomeridiol (1), 4 (15) -eudesmene-1beta, 6alpha-diol (2), 2,5-bornanediol (3), isovanillic acid (4), vanillic acid (5), stigmast-5 (6) , 22 (23)-diene-3beta-ol (6) and beta-sitosterol (7).

CONCLUSIONCompounds 1-5 were isolated for the first time from Gesneriaceae and 6-7 were isolated for the first time from this plant.

Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Magnoliopsida ; chemistry

OBJECTIVETo study the chemical constituents of Reineckia carnea.

METHODThe compounds were separated by means of solvent extraction, repeated chromatogramphy with silica gel, Sephadex LH-20 and MCI resin. The structures were determined by spectral analysis.

RESULTTen compounds were separated and elucidated as daucosterol (1), stigmasterol-3-O-beta-D-glu-copyranoside (2), myo-inositol(3), pentologenin (4), kitigenin (5), kitigenin5-O-beta-D-glu-copyranoside (6), neoaspidistrin (7), (1beta, 3beta, 16beta, 22S)-cholest-5-ene-1, 3, 16, 22-tetrol-1, 16-di-(beta-D-glucopyranoside) (8), rhodeasapogenin 1-0-alpha-L-rhamnopyranosyl-(1-2)-beta-D-xylopyrano-side (9), quercetin-3-O-beta-D-glucopyranosyl-(6-1)-alpha-L-rhamnopyranoside (10).

CONCLUSIONThe compounds 2, 3, 7, 9, 10 were discovered in this plant for the first time.

Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Magnoliopsida ; chemistry

OBJECTIVETo isolate and elucidate the constituents from stem of Chirita longgangensis var. hongyao.

METHODThe constituents were extracted with methanol and isolated by chromatography on silica gel, Sephadex LH-20 and ODS. The structures were determined by NMR and MS spectral analysis.

RESULTSeven compounds were identified as 2-hydroxy-7-methyl-9, 10-anthraquinone (1), 2-methyl-9, 10-anthraquinone (tectoquinone) (2), ursolic acid (3), vanillic acid (4), beta-sitosteryl-D-glucoside-6'-palmitate (5), beta-sitosterol (6), daucosterol (7), respectively.

CONCLUSIONCompounds 1, 2, 3 and 5 were isolated for the first time from the family Gesneriaceae, compounds 4 and 6 were isolated for the first time from the genus Chirita, and compound 7 was isolated for the frist time from Chirita longgangensis var.

Anthraquinones ; chemistry ; isolation & purification ; Magnoliopsida ; chemistry ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Triterpenes ; chemistry ; isolation & purification

OBJECTIVETo study the chemical constituents from Corallodiscus kingianus.

METHODThe column chromatographic techniques were applied to isolate constituents. A combination of ESI-MS and NMR spectroscopy was used to identify structures.

RESULTSix compounds were isolated from the acetone extract of this plant, and the structures of them have been identified as castanopsol (1) , 3beta-hydroxy-9 (11), 12-oleanadien-28-oic acid (2), 2,3,7-trihydroxy-6-oxo-1,3,5 (10), 7-tetraene-24-nor-frie-delane-29-oic acid methylester (3), 3-epicyclomusalenol (4), palmitinic acid (5) and stearic acid (6).

CONCLUSIONCompounds 1-6 were isolated for the first time from Gesneriaceae.

Drugs, Chinese Herbal ; analysis ; Magnoliopsida ; chemistry ; Triterpenes ; analysis

OBJECTIVETo study the chemical constituents from the ethyl acetate portion of an ethanolic extractive of the leaves of Aeschynanthus mengxinensis.

METHODThe column chromatographic techniques were applied to isolate constituents. A combination of IR, ESI-MS, NMR and 2D-NMR spectroscopy was used to identify structures.

RESULTFour compounds were isolated from the ethyl acetate fraction of this plant, and the structure of them have identified as 2alpha, 3beta, 19beta-trihydroxyolean-12-ene-23,28-dioic acid (1), 2alpha, 3beta, 21beta-trihydroxyolean-12-ene-28-oic acid (2), 2alpha, 3beta, 23-trihydroxyurs-12-ene-28-oic acid (3) and stigmast-5 (6), 22 (23)-diene-3beta-ol (4).

CONCLUSIONThe NMR data of compound 1 was completely assigned by 2D-NMR techniques, including HMBC and HMQC. Compounds 1-4 were isolated for the first time from Gesneriaceae.

Drugs, Chinese Herbal ; chemistry ; Magnoliopsida ; chemistry ; Plant Leaves ; chemistry ; Terpenes ; chemistry

To study the sesquiterpenoid constituents in the whole plant of Sarcandra glabra, silical column chromatography, Sephadex LH-20, reverse phase ODS column chromatography and preparative HPLC were used to isolate 70% EtOH extract of Sarcandra glabra. The structures were elucidated based on spectroscopic data (HR-ESI-MS, 1H NMR, 13C NMR, HSQC, HMBC and NOESY). Four sesquiterpenoids were obtained and identified as 4alpha-hydroxy-5alphaH-lindan-8 (9)-en-8, 12-olide (1), chloranthalactone E (2), 8beta, 9alpha-dihydroxylindan-(5), 7 (1)-ieb-8alpha, 12-olide (3) and chloranoside A (4), respectively. Compound 1 is a new sesquiterpene lacone.
Magnoliopsida ; chemistry ; Molecular Structure ; Plants, Medicinal ; chemistry ; Sesquiterpenes ; chemistry ; isolation & purification

OBJECTIVEIsolation and structural elucidation of the constituents of China mangrove Sonneratia apetala.

METHODchromatography methods were used for isolation of compounds, spectroscopic methods were used for structural identifyication.

RESULTseven known compounds named (+/-) symgaresinol, betulinic acid, lupeol, lupeone, stigmast-5-ene-3beta, 7alpha-diol, beta-alpha myrin hexadecaneate, physcoion were isolated.

CONCLUSIONthese known compouns were unreported previously from this plant.

Magnoliopsida ; chemistry ; Pentacyclic Triterpenes ; Plants, Medicinal ; chemistry ; Triterpenes ; chemistry ; isolation & purification