1.Anti-inflammatory and immunoregulatory effects of Yupingfeng powder on chronic bronchitis rats.
Jue SONG ; Jun LI ; Shi-Rui ZHENG ; Yong JIN ; Yan HUANG
Chinese journal of integrative medicine 2013;19(5):353-359
OBJECTIVETo investigate the anti-inflammatory and immunoregulatory effects of Yupingfeng (, YPF) Powder and its components in rats.
METHODSA rat chronic bronchitis (CB) model was developed using lipopolysaccharide (LPS) combined with bacillus Calmette Guerin (BCG). YPF, simple recipe Astragalus membranaceus (Fisch.) Bge (AM) and Astragalus membranaceus (Fisch.) Bge plus rhizome of Atractylodes macrocephala Koidz (AM+RA) decoction were administered (intragastric administration, once a day for 21 days) to rats, to prevent and treat CB. Immunoregulatory and anti-inflammatory effects of YPF, AM and AM+RA were tested by serum pharmacology in vitro on splenic lymphocytes of normal rats and alveolar macrophages of CB rats.
RESULTSInflammation in the pulmonary tissue and the bronchus of CB rats was significantly reduced in the YPF-treatment groups, AM and AM+RA groups demonstrating the efficacy of YPF. Serum samples collected at different times from rats after administration of YPF, AM and AM+RA demonstrated increased proliferation of splenic lymphocytes with area under the effect curve (AUE) of 552.6%, 336.3% and 452.0%, respectively. Treatment of alveolar macrophages with serum samples in YPF, AM or AM+RA group inhibited interleukin-8 (IL-8) in the cell culture media, and the effect was much better in the YPF group compared with AM or AM+RA group, with a higher maximal effect (Emax, P<0.05) and larger AUE (P <0.01 and P<0.05). Moreover, serum from rats treated with AM or AM+RA had similar efficacy, while the efficiency was lower than that treated with YPF.
CONCLUSIONYPF demonstrated anti-inflammatory and immunoregulatory effects in a rat model of CB, and timedependent relationships were demonstrated in vitro.
Animals ; Anti-Inflammatory Agents ; pharmacology ; therapeutic use ; Body Weight ; drug effects ; Bronchitis, Chronic ; drug therapy ; pathology ; Cell Proliferation ; drug effects ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Immunologic Factors ; pharmacology ; therapeutic use ; Interleukin-8 ; metabolism ; Lung ; drug effects ; pathology ; ultrastructure ; Lymphocytes ; drug effects ; Macrophages, Alveolar ; drug effects ; metabolism ; Powders ; Rats ; Rats, Sprague-Dawley ; Spleen ; pathology ; Time Factors
2.Study on the expression of human ERMAP gene in erythropoietic and macrophage differentiation of K562 cells.
Ying-Yi HE ; Xiao-Hong ZHANG ; Tie-Zhen YE ; Zi-Liang WU
Journal of Experimental Hematology 2005;13(4):553-556
In order to investigate the potential of human ERMAP gene in erythroid cell differentiation, K562 cells were induced to erythroid lineage by Ara-C and to macrophage lineage by TPA, human ERMAP mRNA was detected by fluorescent quantitative PCR. The results showed that human ERMAP mRNA increased while K562 cells were induced to erythroid lineage after treatment with Ara-C at 2.5 x 10(-6) mmol/L/L and 1.0 x 10(-6) mmol/L/L. Human ERMAP mRNA not changed while K562 cells were induced to macrophage lineage after treatment with TPA at 2.0 x 10(-6) mmol/L/L and 1.0 x 10(-6) mmol/L/L. It is concluded that human ERMAP gene plays an important role in differentiation and proliferation of erythroid cells.
Antigens, CD
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analysis
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Antigens, Differentiation, Myelomonocytic
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analysis
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Blood Group Antigens
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genetics
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Butyrophilins
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Cell Differentiation
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drug effects
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genetics
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Cytarabine
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pharmacology
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Erythrocytes
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cytology
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metabolism
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ultrastructure
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Flow Cytometry
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Gene Expression
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drug effects
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Humans
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K562 Cells
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Macrophages
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cytology
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metabolism
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ultrastructure
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Microscopy, Electron
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RNA, Messenger
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biosynthesis
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genetics
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Receptors, Transferrin
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analysis
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Reverse Transcriptase Polymerase Chain Reaction
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methods
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Sialic Acid Binding Ig-like Lectin 3
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Tetradecanoylphorbol Acetate
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pharmacology
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Time Factors
3.Downstream components of RhoA required for signal pathway of superoxide formation during phagocytosis of serum opsonized zymosans in macrophages.
Jun Sub KIM ; Jae Gyu KIM ; Chan Young JEON ; Ha Young WON ; Mi Young MOON ; Ji Yeon SEO ; Jong Il KIM ; Jaebong KIM ; Jae Yong LEE ; Soo Young CHOI ; Jinseu PARK ; Jung Han YOON PARK ; Kwon Soo HA ; Pyeung Hyeun KIM ; Jae Bong PARK
Experimental & Molecular Medicine 2005;37(6):575-587
Rac1 and Rac2 are essential for the control of oxidative burst catalyzed by NADPH oxidase. It was also documented that Rho is associated with the superoxide burst reaction during phagocytosis of serum- (SOZ) and IgG-opsonized zymosan particles (IOZ). In this study, we attempted to reveal the signal pathway components in the superoxide formation regulated by Rho GTPase. Tat-C3 blocked superoxide production, suggesting that RhoA is essentially involved in superoxide formation during phagocytosis of SOZ. Conversely SOZ activated both RhoA and Rac1/2. Inhibition of RhoA-activated kinase (ROCK), an important downstream effector of RhoA, by Y27632 and myosin light chain kinase (MLCK) by ML-7 abrogated superoxide production by SOZ. Extracellular signaling-regulated kinase (ERK)1/2 and p38 mitogen-activated protein kinase (MAPK) were activated during phagocytosis of SOZ, and Tat-C3 and SB203580 reduced ERK1/2 and p38 MAPK activation, suggesting that RhoA and p38 MAPK may be upstream regulators of ERK1/2. Inhibition of ERK1/2, p38 MAPK, phosphatidyl inositol 3-kinase did not block translocation of RhoA to membranes, suggesting that RhoA is upstream to these kinases. Inhibition of RhoA by Tat-C3 blocked phosphorylation of p47 PHOX. Taken together, RhoA, ROCK, p38MAPK, ERK1/2, and p47 PHOX may be subsequently activated, leading to activation of NADPH oxidase to produce superoxide.
Animals
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Cell Line
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Cell Membrane
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Cytosol
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Enzyme Inhibitors/pharmacology
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Extracellular Signal-Regulated MAP Kinases/metabolism
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Macrophage-1 Antigen/pharmacology
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Macrophages/drug effects/*metabolism/ultrastructure
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Mice
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Myosin-Light-Chain Kinase/metabolism
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Opsonin Proteins/blood/*metabolism
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*Phagocytosis
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Protein Transport
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Protein-Serine-Threonine Kinases/metabolism
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Research Support, Non-U.S. Gov't
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*Signal Transduction
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Superoxides/*metabolism
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Tetradecanoylphorbol Acetate/pharmacology
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Zymosan/*blood
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p38 Mitogen-Activated Protein Kinases/metabolism
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rhoA GTP-Binding Protein/antagonists & inhibitors/*metabolism