ObjectiveTo investigate the change of Bcl-2 and P53 expresssion after whiter matter damage in neonatal rats. Methods100 rats (2 days old) were randomly divided into two groups: control group and periventricular leucumalacia (PVL) group. Control group received normal saline 5 mg/kg intraperitoneal injection, PVL group received lipopolysaccharide (LPS) 5 mg/kg intraperitoneal injection. The expression of Bcl-2 and P53 was measured 6 h, 12 h, 24 h, 48 h after operation with immunohistochemical staining. ResultsThe expression of Bcl-2 increased 6 h after operation, and to the top 48 h after operation in the PVL group, which was significantly different compared with the control group (P<0.01). The expression of P53 increased at 6 h after operation, and to the top 12 h after operation in the PVL group, which was significantly different compared with the control group (P<0.01). ConclusionThe expression of Bcl-2 and P53 increased in the white matter injuried neonatal rats induced by LPS.

The article reviewed the common analysis methods, and application status of the surface electromyography (sEMG) in the assessment and treatment of child neurological rehabilitation.

Objective To assess and compare physical and chemical properties of skin among different body areas of healthy children at different ages from Beijing.Methods A total of 116 healthy children were recruited from Beijing,China,and classified into four age groups.Tewameter TM300 was used to measure transepidermal water loss (TEWL) value,Corneometer CM825 to estimate stratum corneum hydration,and skinpH-Meter PH905 to determine skin surface pH,in the forehead,cheek and forearm.The Mexameter MX 18 probe to determine melanin index of the face.Analysis of variance was carried out to assess differences in these parameters among different measurement sites and age groups.Results TEWL value did not differ among different age groups or measurement sites (both P ＞ 0.05).The stratum corneum hydration value of forehead,cheek and forearm was 51.53 ± 15.70,39.88 ± 10.48 and 50.33 ± 17.54 respectively in the age group ＜ 1 year,49.95 ± 17.88,32.51 ± 12.09 and 36.10 ± 7.43 respectively in the age group 1-3 years,51.37 ± 10.60,31.65 ± 9.01 and 34.41 ± 8.21 respectively in the age group 4-6 years,49.74 ± 10.64,39.99 ± 50.43 and 29.35 ± 8.10respectively in the age group 7-12 years,with significant differences among different measurement sites (P ＜ 0.05) but not between different age groups (P ＞ 0.05).The cheek and forearm showed lower stratum corneum hydration value than the forehead.No statistical difference was observed in pH value at the same measurement sites between the age group ＜ 1 year (forehead 5.27 ± 0.60,cheek 6.12 ± 0.51 and forearm 5.48 ± 0.45),1-3years (forehead 4.68 ± 0.58,cheek 6.80 ± 0.55 and forearm 5.07 ± 0.58),4-6 years (forehead 4.58 ± 0.37,cheek 5.70 ± 0.48 and forearm 5.09 ± 0.49),and 7-12 years (forehead 4.87 ± 0.51,cheek 5.72 ± 0.49 and forearm 5.09 ± 0.51),but the cheek had significantly higher pH value than the forearm and forehead (both P ＜ 0.05).Melanin value on the face did not differ between different age groups (P ＞ 0.05).Conclusion Physical and chemical properties of skin vary with body sites in healthy children.

Child ; Female ; Humans ; Job Syndrome

Objective To investigate the clinical effects of continuous 3-in-1 femoral nerve block with stimulating catheters for patient controlled regional anesthesia(PCRA) in elderly patients after unilateral total knee replacement (TKR)surgery.Methods Thirty ASA Ⅰ - Ⅱ elderly patients wererandomly divided into two groups: FB group and Ⅳ group.FB group received continuous 3-in-1 femoral nerve block for postoperative analgesia with 0.2% ropivacaine plus 0.1 μg/ml sufentanil continuous infusion at 5 ml/h plus PCA boluses (1.0 ml/15 min).Ⅳ group received continuous intravenous analgesia with 1 μg/ml sufentanil plus 0.04 mg/ml tropisetron hydrochloride at 2 ml/h plus PCA boluses (0.5 ml/15 min).All patients were maintained analgesia for 48 hours.Results In FB group, the visual analogue scale(VAS) scores were 1.3 ±1.1, 1.2 ± 1.0, 1.1±0.9, 1.1 ± 1.0,1.0±0.9 at 4, 8, 12, 24, 48 hours after surgery under rest status respectively and were 3.04±1.4,2.3±1.3 at 24, 48 hours after surgery in active function training.These parameters in Ⅳ group were 4.0±1.6, 3.5±1.6, 3.2±1.4, 3.0±1.3, 2.5±1.2, 4.7±1.5 and 3.3±1.5 respectively, which were significantly different compared with FB group (t=5.358, 4.707, 4.852, 3.784, 3.743, 3.254,1.932,all P<0.05 or P<0.01).The incidence of nausea was higher in IV group than in FB group(P = 0.0022).Postoperative satisfaction ratings was higher (χ2 =41.1 ,P<0.01) and the total morphine use for 48 hours after operation was less(uc=2.412, P<0.01) in FB group than in Ⅳ group.Conclusions After TKR surgery, the continuous 3-in-1 femoral nerve block with stimulating catheters is an effective method with better pain relief,fewer side effects and higher satisfaction ratings in the elderly.

ObjectiveTo evaluate morphometric changes of brain in patients with alcohol addiction by voxel-based morphometry. MethodsFifteen patients with alcohol addiction and 15 health controls were recruited and underwent fluid attenuated inversion recovery (FLAIR) and 3D fast spoiled gradient echo (FSPGR) T1 -weighted sequences on a 3.0 T MRI system. 3D FSPGR T1 structure images were normalized,segmented and smoothed, and then underwent voxel-based morphometry. An ANCOVA was applied with age,body mass index ( BMI ), and education years as covariates because of exact sex match.A statistical threshold of P ＜ 0. 01 (uncorrected) and more than continuous 50 voxels were determined as significant.ResultsRegional results showed there was a significant reduction of gray matter volume in left middle frontal gyrus, superior frontal gyrus, precuneus, gyrus rectus, right medial superior frontal gyrus and orbital gyrus in patients with alcohol addiction compared with health controls, and the regional reduction of white matter volume in patients with alcohol addiction mainly located in the white matter of bilateral superior frontal gyrus. Global brain volume analysis showed there was a significant smaller volume in white matter [ (675. 14 ± 70. 62) ml]and brain parenchyma [ ( 1578. 81 ± 199. 05 ) ml]for patient group than that in white matter [ ( 841.33 :± 177. 35 ) ml]and brain parenchyma [ ( 1722. 13 ± 117.62) ml]for control group (t ＝ 11.37,5. 764, respectively, P ＜0. 05 ), but there was no difference in the gray matter volume between patient group [(903.67 ±1±9.87) ml]and control group [(880.79 ±1±7.10) ml](t ＝0.177, P＞0. 05 ). ConclusionsRegional gray and white matter atrophy can be the initial changes in patients with alcohol addiction and the frontal region is a relative specific damaged brain region.VBM has a potential value for the detection of subtle brain atrophy in patients with alcohol addiction.

Cytokines ; Hashimoto Disease ; diagnosis ; prevention & control ; Humans

Objective To investigate the clinical diagnostic value of transperineal ultrasound in women in late pregnancy with stress urinary inconti?nence. Method Totally 66 cases of women in late pregnancy were selected,who did not suffer from SUI(groupⅠ),and 54 cases of women in late pregnancy who suffered from SUI(groupⅡ). Another 63 cases of healthy childless women were used as normal control(groupⅢ). Transperineal ultrasound was used to dynamically observe their pelvic floor structure,and the ultrasonic parameters were recorded and analyzed,including the posi?tion of bladder neck in X axis and Y axis((Djx,Dzx,Djy,Dzy)during resting period and tension period,the vesicourethral angle(αj,αz),the Bladder angle(βj,βz). The moving of the bladder neck in X axis(ΔDx=Dzx-Djx)and Y axis(ΔDy=Dzy-Djy),as well as the bladder rotation an?gle(Δβ=βz-βj)were calculated and the movement of the bladder neck(ΔD)was measured,followed by comparative analysis. The threshold val?ues of all ultrasonic parameters were determined by the ROC curve analysis. Results The bladder neck of all three groups were backward during tension period. Compared with groupⅢ,ΔDy,Djx,Dzx obviously decreased in groupⅠand groupⅡ,whileβj,βz,αj increased significantly.ΔDx of groupⅡwas significantly larger than that of groupⅢ(P<0.05);There was significant difference in Djy,Dzy,ΔD,Δβandαz among the three groups(P<0.05);In ROC curve analysis,when the critical value of Djy,Dzy,ΔDy,ΔD,αz andΔβwas-1.75 cm,-1.71 cm,0.25 cm,0.29 cm,131.5° and 3.5° ,their sensitivity was 88.9%,88.9%,72.2%,83.3%,88.9%and 72.2%,and the specificity was 72.2%,81.8%,72.7%, 95.5%,86.3%and 68.2%,respectively. Conclusions The transperineal ultrasound could clearly show the pelvic floor of the women in late preg?nancy,the bladder urethral support structure damage or defect was more serious in the women in late pregnancy who were suffering from SUI,and the determination of the critical values of all ultrasonic parameters helped the screening of SUI in women in late pregnancy.

Isopinosine (ISO, 25?100 mg/kg?d-1ip for 4 or 5d) significantly increased the production of serum hemolysin-and spleen plaque forming cell ( PFC) in normal mice immunized with SRBC. The same result was observed in immunosuppressive mice induced by cy clophospha-mide. In 16 month aged mice, ISO (ip for 5 d) showed potentiating effect on anti-SRBC PFC response at the dose of 2 .5 mg/kg, no significant effect at the dose of 5 .0 mg/kg, and inhibiting effect at the dose of 25 and 100 mg/kg respectively. 1SO(25,50 and 100 mg/kg?d-1 ip for 9 or 10 d ) remarkably enhanced delayed cutaneous hypersensiti-vity (DCH) to 2, 4 -dinitrochlorobenzene (DNCB) in normal mice as well as in immunosuppressive mice induced by cyclophospha-mide. ISO (5, 10 and 20 mg/L ) signficantly increased ConA-induced lymphocyte proliferation of spleen cells from C57BL/6J mice in vitro. There was also potentiating effect on lymphocyte proliferation induced by ConA in 16 month aged mice.