1.The epidemiological and pathogenic detection characteristics of 2019 novel coronavirus in children
Chinese Journal of Laboratory Medicine 2021;44(4):285-290
As a new emerging infectious virus, 2019 novel coronavirus (2019-nCoV) has been widely popular all over the world since the beginning of 2020. As a special group, children have similarities and differences with adults for coronavirus disease 2019 (COVID-19) in the epidemiology, diagnosis and treatment, curative effect monitoring, prognosis, virus testing methodology, infection and inflammation markers, etc. This paper mainly reviews the characteristics of COVID-19 in children from two aspects: the epidemiological and pathogenic detection characteristics, so as to provide some basis for its diagnosis and treatment.
2.Detection of trace elements in children and its clinical value
Chinese Journal of Laboratory Medicine 2016;39(4):240-242
While only in minute quantities in the body, trace elements are crucial co-factors and regulators of normal metabolism.Commonly tested elements include calcium, magnesium, iron, zinc, and copper.Imbalance or deficiencies in trace elements can cause growth retardation, malnutrition, immune malfunction and other abnormalities in children.Because these elements are in trace amounts, sensitivity and reproducibility of detection methods are of paramount importance.Different methods for the same element can yield different reference intervals, which, in order to be applied in the clinic, would have to be independently established by individual laboratories.Clearly defined indications and good quality control to ensure accuracy and sensitivity are necessary for the measurement of trace elements to provide objective reference for clinical diagnosis.
3.Treatment of drug-induced severe hepatitis with hepatocyte transplantation
Lin ZHOU ; Yongping YANG ; Wei MA
Medical Journal of Chinese People's Liberation Army 2001;0(10):-
Objectives To evaluate the efficacy and safety of h um an hepatocyte transplantation in the treatment of severe hepatitis induced by dr ugs. Methods The primary human hepatocytes were isolated from t he liver of a healthy donor, and they were then cryopreserved. The thawed hepato cytes were transplanted into the patient's spleen via a femoral artery catheter. 2?10~10 hepatocytes were harvested, and 70% of thawed hepatocytes were vi able, and 2?10~9 vital hepatocytes were transplanted. Results One month after the transplantation, clinical symptoms of the recipient were a meliorated obviously, and the levels of BIL, NH3, ALT and AST lowered, while PA elevated. 50 days after discharge from the hospital it was found that biochemica l parameters returned to normal values, and the hepatocyte signal could be detec ted in the spleen with MRI. Conclusion Hepatocyte transplantati on is safe and efficacious.
4.Clinical observation on the treatment of severe hepatitis with hepatocyte transplantation
Yongping YANG ; Lin ZHOU ; Wei MA
Medical Journal of Chinese People's Liberation Army 1982;0(01):-
Objective To investigate the effects and safety of human hepatocytes transplantation in vivo for the treatment of liver failure. Methods The primary human hepatocytes were collected from normal liver tissue donated by healthy volunteers and preserved by cryopreservation technique. After thawing, the hepatocytes were transplanted into the spleen of patients with severe hepatitis through catheterization of the femoral artery. Then the changes in clinical symptoms, serum biochemical indexes and MRI signals of the spleen were observed in the patients. Results A total of 2?10 10 hepatocytes were isolated from normal liver tissue of healthy volunteers and 75% of the hepatocytes were alive after cryopreservation and thawing. The number of transplanted hepatocytes was 2?109. In the recipients, the clinical symptoms were markedly improved, serum levels of bilirubin, NH_3, ALT and AST were significantly reduced, but that of PTA remarkably increased, after hepatocyte transplantation. The follow-up examination was performed 80d and 270d after discharge from the hospital, and it was showed that all the serum biochemical indexes returned to normal and signals of the hepatocytes were found in the spleen. Conclusions Hepatocyte transplantation is a safe and effective therapy for severe hepatitis. The transplanted hepatocytes can proliferate and differentiate in the spleen to replace or partially compensate the liver function of synthesis, detoxication and metabolism. Contrast enhanced MRI can be a new method for follow-up study of transplanted hepatocytes.
5.Analysis of 12R-lipoxygenase gene mutations in three families with self-improving collodion ichthyosis
Chinese Journal of Dermatology 2021;54(5):397-401
Objective:To analyze gene mutations in 3 families with self-improving collodion ichthyosis.Methods:Clinical data were collected from 3 patients with self-improving collodion ichthyosis. DNA was extracted from the peripheral blood of patients and their parents, and high-throughput sequencing was performed in the patients by using a multi-gene panel targeting congenital ichthyosis. After identification of causative gene loci, Sanger sequencing was performed to bidirectionally verify the mutations in the patients and their parents.Results:All the 3 patients presented with a collodion-like membrane at birth, which was shed within 2-4 weeks after birth, and then they gradually showed similar features of mild ichthyosis, including dry skin, tiny scales at local sites, flexural involvement, mild sweating, heat intolerance, cheek flushing, mild palmoplantar keratosis or palmar hyperlinearity. Compound heterozygous mutations were identified in the ALOX12B gene of the 3 patients, including a paternal mutation c.406_408delGAG and a maternal mutation c.77T>C in case 1, a paternal mutation c.1013C>T and a maternal mutation c.1286C>G in case 2, a paternal mutation c.1232T>C and a maternal mutation c.1440C>A in case 3. Function prediction analysis showed that 4 missense mutations c.77T>C, c.1286C>G, c.1013C>T, c.1232T>C and 1 deletion mutation c.406_408delGAG may exert pathogenic effect, and 1 nonsense mutation c.1440C>A led to the generation of a termination codon encoding a truncated protein p.Tyr480Ter, which may affect the protein function and cause disease. None of the 6 mutation sites had been reported in the past.Conclusion:Compound heterozygous pathogenic mutations were identified in the ALOX12B gene of the 3 patients with self-improving collodion ichthyosis, which were inherited from their parents.
6.Transcription activator protein 1 (AP-1)-related study in basophils from patients with chronic idiopathic urticaria
Yiping MA ; Xu YAO ; Lin LIN ; Wuqing ZHOU ; Pangen CUI
Chinese Journal of Dermatology 2010;43(11):762-765
Objective To investigate the role of AP-1 in the pathogenesis of chronic idiopathic urticaria (CIU). Methods By using immunomagnetic separation technology, peripheral blood basophils were isolated from 10 CIU patients and 10 normal human controls followed by the extraction of nuclear protein from the basophils. TransAMTM AP-1 family kit was used to detect the DNA binding activity changes of AP-1 family transcription factors in basophils, and Western blotting to detect the expression of P-c-jun protein. Results There were some differences in the DNA binding activity of AP-1 family transcription factors in basophils between CIU patients and normal controls. The DNA binding activity of Phospho-c-jun, c-fos, Fos-B, Jun-B and Jun-D factors was increased in CIU patients compared with the controls, and the increase in that of P-c-jun and Jun-D was statistically significant (both P < 0.05). There was an insignificant decrease in the DNA binding activity of Fra-1 factor in the CIU patients compared with the controls (P > 0.05). The P-c-jun (Ser73) protein expression was higher in CIU patients than that in the controls (0.527 ± 0.312 vs. 0.435 ± 0.042, P < 0.05),whereas there was no significant difference in the P-c-jun (Ser63) protein expression level. Conclusion Some changes in DNA binding activity of AP-1 and overexpression of P-c-jun (Ser73) protein in basophils may be involved in the pathogenesis of CIU.
7.Antitumor activities of chemical constituents of Cephalotaxus fortunei in Guizhou province
Mei ZHOU ; Lin MA ; Xiaojiang HAO ; Xiaosheng YANG
Journal of China Pharmaceutical University 2009;40(3):209-212
Aim: To study the antitumor activities of the constituents of Cephalotaxus fortunei distributed in Guizhou province. Methods: The constituents were isolated by column chromatography and identified by physical and spectral analysis. Meanwhile, the anti-tumor activities of some compounds were evaluated by sulforhodamine B( SRB) and MTT assay. Results: Eleven compounds were isolated and identified as apigenin (Ⅰ), β-sitosterol (Ⅱ), acetylcephalotaxine (Ⅲ), chrysoeriol (Ⅳ), drupacine ( Ⅴ), 1-hentriacontanol ( Ⅵ), 7, 3', 4' -trihydroxyfla-vone (Ⅶ), sugiol ( Ⅷ), cephalotaxine (Ⅸ), wllsonine (Ⅹ), and hainanolide (Ⅺ), respectively. Biological screening results demonstrated that some of the tested compounds exhibited the antitumor activities in vitro. Conclusion: Compounds Ⅱ, Ⅵ-Ⅷ were isolated from this plant for the first time. Compound Ⅺ has a better inhibitory activity on cell line A549 and K562 .
8.The diagnosis value of multi-sequences magnetic resonance imaging in recurrent pyogenic cholangitis
Gaozheng PAN ; Zhao MA ; Yijun LIU ; Lin ZHOU
Journal of Practical Radiology 2014;(10):1683-1686
Objective To investigate the clinical value of the combination of routine magnetic resonance imaging (MRI),magnetic resonance cholangiopancreatography (MRCP),diffusion weighted imaging (DWI)and enhanced magnetic resonance imaging in the diagnosis of recurrent pyogenic cholangitis (RPC).Methods The clinical and MRI data of 2 1 cases of RPC confirmed by surgery or puncture were retrospectivly analyized.Results All of 2 1 patients were performded with routine MRI (FSE T2 WI,FSPGR T1 WI and FIASTA),MRCP and DWI.1 2 cases were underwent three-dimensional dynamic contrast-enhanced MRI with a liver volume accelerated acquisition sequence (LAVA).The raw data was uploaded to GE ADW4.4 station and the three dimensional MRCP and DWI were analyzed.(1)All of 21 cases were hepatolithiasis complicated with irregular expansion.11 cases were involved left intra-hepatic bile duct,7 cases were right intrahepatic duct,and 3 cases were involved left and right intrahepatic bile duct.Wall thickening was in 19 cases,normal biliary duct wall was in 2 cases.(2)Common bile duct disease:calculus of intrahepatic and extrahepatic bile duct complicated with bile duct dilatation were in 8 cases.Calculus of intrahepatic bile duct were individually in 13 cases.(3)Gall-bladder disease:there were 5 cases of gallstone,9 cases of chronic cholecystitis,3 cases of acute cholecystitis and 4 cases of chole-cystectomy.(4)Liver parenchyma disease:there were 6 cases of liver atrophy in all the cases (left lobe atrophy of 4 cases,right lobe atrophy of 4 cases).The inflammatory changes around dilated bile ducts were found in 1 2 cases.The MRI findings of those were slight patchy hyperintensity signal on conventional T2 WI,the area of hyperintensity signal on T2 WI was extended on DWI.On dynamic contrast-enhanced MRI,the inflammatory areas were uniformity minor enhancement on arterial phase,and hardening in-creases on portal and delayed phase.Conclusion Multi-sequences MRI can be more fully reflected the pathologic changes of RPC, which is important for imaging diagnosis of the disease.
9.Prospective randomized clinical trial of non-small cell lung cancer for intraoperative radiotherapy
Guixia ZHOU ; Diwen ZENG ; Lianyuan WANG ; Lin MA
Chinese Journal of Radiation Oncology 1992;0(01):-
Objective To analyse the long-term effect of non-small cell lung cancer treated with either postoperative irradiation(group A) or intraoperative radiotherapy(IORT) followed by postoperative irradiation(group B).Methods 154 patients with non-small cell lung carcinoma were randomized into two groups(groups A and B) with 77 patients in each.There were 134 squamous,17 adenocarcinoma and 3 adeno-squamous carcinoma.Seventeen patients had stage Ⅰ,76 stage Ⅱ and 61 stage Ⅲ lesions.The dose of postoperative irradiation in both groups was D_T4060?Gy.In group B,the IORT dose was 15-25?Gy,delivered by 9-16 ?MeV electrons.Results The local control rate was 49% and 62% in groups A and B,respectively(P0.05).Sixteen patients died of radiotherapy-induced complication: 2 in group A and 14 in group B.Conclusions Intraoperative radiotherapy followed by postoperative irradiation can enhance the local control rate of non-small cell lung cancer but can not improve the long term survival. The high complication mortality rate of IORT(18%) in contrast to that(3%) of postoperative radiotherapy is worth noticing.
10.Research progress on effects of traditional Chinese medicines on proliferation, apoptosis and differentiation of bone marrow mesenchymal stem cells.
Jian-Kang FANG ; Yi-Ping ZHOU ; Ma-Lin LI
China Journal of Chinese Materia Medica 2014;39(15):2834-2837
Bone marrow mesenchymal stem cells (BMSCs) are a kind of pluripotent stem cells derived from bone marrows, which can not only support hematopoiesis, but also have capabilities of multidifferentiation, high-proliferation and self-renewing. They have become one of hotspots in stem cell studies. Studies on in vitro intervention with BMSCs with TCMs have made remarkable progress in recent years. According to the findings, some traditional Chinese medicines can promote proliferation of BMSCs, some can inhibit the apoptosis of BMSCs, while others can induce BMSCs to differentiate into multiple cell types, such as osteoblast. Furthermore, some studies also involved relevant action mechanisms. The authors summarized the advance in relevant studies by reference to relevant literatures of this field.
Animals
;
Apoptosis
;
drug effects
;
Bone Marrow Cells
;
cytology
;
Cell Differentiation
;
drug effects
;
Cell Proliferation
;
drug effects
;
Humans
;
Medicine, Chinese Traditional
;
methods
;
Mesenchymal Stromal Cells
;
cytology
;
drug effects