Objective To observe the effect of ganglioside GM3 on the proliferation and cell cycle of multiple myeloma (MM) cell line U266. Methods The experimental groups were treated with 20, 40, 80, 160μmol/L GM3, while the control group was not given GM3. The growth inhibition effect of GM3 on U266 cells after 48 hours were measured by using methyl thiazolyl tetrazolium (MTT) method. The cell cycle was tested by flow cytometry with PI labeling. Results The results indicated that the GM3 displayed anti-proliferative effects on U266 cells in a dose-dependent manner. The cell inhibition rates were (13±4) %, (26± 4) %, (47 ±6) %, (55 ±10) % in different dose of GM3 (20, 40, 80, 160 μmol/L), respectively. There was a difference between the experimental group and the control group (F= 93.063, P< 0.05). At the same time, the cell cycle analysis results showed that the U266 cell ratio in S phase was increased from (22.6 ±3.7) % to (71.5±3.8) %(P<0.01) and in G2/M phase was decreased from (42.6±2.5) %to (0.8±0.6) %(P<0.05) while in G0/G1 phase was not significantly changed. Conclusion GM3 can inhibit the proliferation of MM cell line U266 by blocking them in the S phase.

The 14th International Conference on Malignant Lymphoma (ICML) was held in Lugano, Switzerland from June 14, 2017 to June 17, 2017. More than 3500 lymphoma experts and scholars attended this unprecedented conference. In particular, the Joint Forum on Union for China Lymphoma Investigators (UCLI)-ICML jointly organised by UCLI and ICML had greatly improved Chinese academic status in the field of lymphoma worldwide. Chinese experience on the treatment of T-cell lymphoma was exchanged during the conference. This paper reviews the recent progress in treatment of T-cell lymphoma in the meeting.

Objective The research of craniocerebral injury and fracture healing has made slow progress, and the lack of reliable animal experimental model is one of the key factors.Therefore, the focus is to establish a craniocerebral injury and fracture model up to standard.The study aims to establish an appropriate animal model and evaluate the stability.Methods New Zealand white rabbits were randomly divided into four groups(n=12): control group, simple craniocerebral injury group(simple craniocerebral injury model), simple fracture group(simple right tibial fracture model), Brain trauma with fracture group (craniocerebral injury combined with fracture model).The neurological changes of the rabbits were observed.Furthermore, the healing of the fractures was observed at different time postoperatively by measuring the volume of callus and observing pathology.Results The neurological function scale (NSS) scores [(9.32±0.68), (8.01±0.56), (7.75±0)]6 h, 7 d and 1 month after craniocerebral injury surgery were significantly lower than those in the control group[(11.81±0.55),(12.07±0.42),(12.02±0.38)], and there was statistically significant difference (P<0.05).Compared with the callus volume of simple fracture group around 1 month after surgery, the callus volume of the craniocerebral injury combined with fracture group was significantly larger [(1.19±0.12) cm3 vs (1.81±0.13) cm3, P<0.05].Four weeks after surgery in the craniocerebral injury combined with fracture group, the trabecular bone was significantly increased in the local callus.After 8 weeks, the trabecular bone became more mature.Conclusion The animal model of craniocerebral injury combined with fracture healing is stable and reproducible.It meets the standard for studying the relationship of traumatic brain injury and fracture.

Objective To establish the evaluation system and explore the possible impact factors for public interest nature in public hospitals. Methods One hundred and twenty-five public hospitals in Zhejiang Province were served as sample hospitals, and related data of year 2004 to year 2006 were collected. The Delphi expert investigation method, variation coefficient method and TOPSIS method were employed to screen the impact factors for public interest nature and build up the evaluation system for public hospitals, and stepwise regression analysis was conducted for possible impact factors of public interest nature. Results The evaluation system mainly included five aspects of quantity, quality, efficiency, mean charge of medical service and social satisfaction to public hospitals. The two most important factors that influenced the public interest nature were the number of beds and the number of equipments valued above 10 000 RMB. Conclusion The hospital scale is the most important impact factor for the nature of public interest in public hospitals. Therefore, the scale of public hospitals should be properly designed so as to ensure the public interest nature in public hospitals.

The 13th International Conference on Malignant Lymphoma (ICMC) was held in Lugano, Switzerland from June 17 to 20, 2015.There was the enormous progress in the treatment of chronic lymphocytic leukemia in the past years, especially in 2014, 4 new agents for the treatment of chronic lymphocytic leukemia were approved in USA and Europe, which caused great attention on the issue of chronic lymphocytic leukemia.The latest progress of chronic lymphocytic leukemia reported at the conference was briefly introduced in this paper.

In recent years, immunotherapy has become an indispensable part in treatment of cancer, and the immune checkpoint inhibitors are the focus of attention. In the 58th American Society of Hematology (ASH) Annual Meeting, the application of immune checkpoint inhibitors in hematologic malignancies had been widely concerned. The recent progress of immune checkpoint inhibitors in hematologic malignancies in this annual meeting will be briefly introduced.

Ultrasound molecular imaging has become one of the hotspots of molecular imaging because of its advantages of non-invasion, non-radiation, repeatability and real-time dynamic imaging. The key of imaging is to select the appropriate target and ligand to bind to the ultrasound contrast agent. The formation of blood vessels in the development of tumor is a significant feature. Vascular endothelial growth factor receptor (VEGFR) is an important specific molecule on tumor vascular endothelial cells, and which can be used as a target for tumor ultrasound imaging. In recent years, many scholars have carried out related studies on the imaging of ultrasound molecules targeting VEGFR. This paper reviewed the mechanism and application of tumor ultrasound molecular imaging with VEGFR as target in recent years.

Objective To analyse the economies of scale in public hospitals in Zhejiang Province. Methods One hundred and twenty-five public hospitals in Zhejiang Province with qualification no less than class two level two were selected as sample hospitals. Douglas production function was employed to analyse the relationship between investment and output in sample hospitals between 2004 to 2006, with two types of production models established by medical service and incomes in hospitals as dependent variables. Results From 2004 to 2006, the medical service and incomes of sample hospitals were in line with economies of scale. The production increase rate was higher than investment increase rate. The medical service, incomes, total assets and number of staff in class three hospitals were higher than those in class two hospitals (P =0.000). Conclusion Economies of scale is the ultimate incentive to continuous outstretch for hospitals. Hospitals should rationally control the scale so as to avoid problems in medical service caused by overstretch.

RNA interference which focuses on siRNA becomes a studying focus in malignant tumor of gene therapy due to its high efficiency and effect, the sequence of specific characteristics and action fast. In recent years many research shows that constructing and transfecting malignancy related gene targeting specific siRNA, can effectively suppress tumor growth and invasion. Combination with other conventional treatments can improve the curative effect. It's a promising new method of gene therapy in head and neck neoplasms.

This article summarized literatures in the recent ten years on the mechanisms of Ci-xue therapy from such aspects as hemorheology, vascular biology, blood constituents and blood coagulating system.