Objective:To evaluate and analyze the application value of Acute Physiology and Chronic Health EvaluationⅢ(A- PACHEⅢ)in evaluation of the severity and prognosis of patients in SCIU.Methods:By collecting and sorting out the data of 341 cases of surgical serious patients,the severity and prognosis of patients were evaluated by APACHEⅢ.Results:Making comparison among the different types of diseases,the scores and mortality of cerebral hemorrhage and multiple hurt were high- er than those of the diseases(P

Objective: To study the marriage quality of different occupational groups. Methods: 200 married teachers from two universities and 144 married employees in hospitality industry in Hainan Province were applied Olson 's Questionnaire on Marriage Quality. Results: First, the total score of the teachers was higher than that of the employees (35. 1?6. 7/33. 5 ?6. 6, t =5. 59, P =0. 00) . Marriage satisfaction, character compatibility, communications between husbands and wives, while the arrangements of their incomes and leisure activities were also higher in teachers (t = 2. 20 -3. 37, P

Objective:To investigate the value of YL-1 puncture hematoma evacuation for hypertensive intracerebral hematoma.Methods:138 patients with hypertensive intracerecbral hematoma treated by YL-1 puncture hematoma evacuative were studied retrospectively,and the relationship between the age,the time of operation,GCS and volume of hemorrhage with mortality were with logistic regression method analysis.Results:①There were no significant relation in all the ages;②the shorter the hemorrhage,the higher the mortality;③the lower GCS was,the higher the mortality was;④the bigger the hemorrhage volume,the higher the mortality.Conclusion:The present study indicates that age is not an factor to limit minimally invasive operation(YL-1 puncture hematoma evacuation).The effect of surgery depended on the time of hemorrhage,GCS and volume of hemorrhage.

Objective To study clinical characteristics of the thalamic hemorrhage,probe into the correlation factor of judged prognosis in early days and case-fatality rate.Methods To 2003—2005 96 thalamic hemorrhage sufferer's clin- ical data were analysed.Results The advanced age and high blood pressure is main cause of disease of the thalamic hemorrhage.Conclusion To observe the thalamic hemorrhage sufferer's vital signs have the certain reference value to judged prognosis,case-fatality rate and serious complications were related to bloods closely in early days,whether break into the brain room,hypothalamus and the brain stem whether implicate.

Objective To understand the clinical medicine graduates competency status of Kunming Medical University in the past 5 years and provide important reference for cultivating students in medical university. Methods The questionnaire results in the relevant departments responsible person of Yunnan provincial municipal and district 26 hospitals were system analyzed by fuzzy comprehensive evaluation method. Results In the past 5 years, the graduates occupation competence of clinical medicine in Kunming Medical University is good. Among them, the graduates’core competency gets the lowest score, but the graduates’post competency gets the highest score. Conclusion The graduates’core competency should be strengthened in training of doctor-patient communication skills and quality evaluation of clinical practice for medical students.

The paper analyzes the impact of MOOC on medical information resources construction,and proposes the response strategies for the information resources construction of medical library,including introducing Patron Driven Acquisitions (PDA) mode,strengthening digital resources construction efforts,developing medical MOOC course reference resources system and constructing medical library alliance.Meanwhile,it points out that the medical library is required to dig users'information demand further and solve the issue of MOOC copyright clearance.

Many college students’ English is not good enough to meet the need of the society. Pairing general English with ESP is proposed so as to solve the problem. Taking college English teaching in schools of traditional Chinese medicine as a case, this paper analyzes the key factors such as teaching materials, language teachers, students, teaching objectives, methods and environment in the teaching process in order to prove that pairing general English with ESP is feasible.

Objective:To lay foundation for the functional studies of programmed cell death 5 ( PDCD5 ) and develop new technical pathway for bioinformatics analysis of human functional genes. Methods:Using PDCD5 as the target molecule, intensive bioinformatics analysis of the nucleic acid and protein sequences were conducted. Data mining and comprehensive analysis by sequence against database similarity searching, ortholog structure comparison, expression profile analysis and gene “neighbor” listing were performed. Results: Two human putative pseudogenes on chromosomes 12 and 5, and one mouse putative pseudogene on chromosome 1 were identified. The methanobacterium thermoautotrophicum ortholog was classified as the same fold as ubiquitin and ribosomal protein S13. The C. elegans ortholog, ubiquitin and IAP (inhibitor of apoptosis proteins) belonged to the same expression profile cluster. This cluster was related to biosynthesis and protein synthesis. PDCD5 orthologs in various genomes were adjacent to various ribosomal proteins on the chromosome. Conclusion: The human genome contains at least two processed pseudogenes of PDCD5 . Besides the relationship with cell apoptosis, PDCD5 is predicted to have functional relationship with ubiquitin and participate in the translation regulation.

OBJECTIVE:To establish the method for simultaneous determination of 5 heavy metals in Vespae Nidus,and to dis-cuss the effects of difterent producing area and part on the contents of heavy metals in Vespae Nidus. METHODS: The contents of arsenic(As),mercury(Hg),lead(Pb),cadmium(Cd)and copper(Cu)were determined by atomic spectrophotometry(hydride generation method,cold vapor method,graphite furnace method and flame method). The difference in the contents of heavy metals in samples were compared among different producing areas by analyzing data. RESULTS:The linear range of As,Hg,Pb,Cu, Cd were 0-40 ng/mL(r=0.9930),0-0.9 ng/mL(r=0.9974),0-80 ng/mL(r=0.9955),0-8 ng/mL(r=0.9927),0-800 ng/mL(r=0.9966),respectively. Limit of quantitation were 4.473,2.35×10 - 3 ,8.380,2.128,54 ng/mL,limit of detection were 1.342,0.701× 10 - 3,2.514,0.632,16 ng/mL. RSDs of precision,stability and reproducibility tests were all lower than 5.0%. The recoveries were 84.26% -102.25%(RSD=7.71% ,n=6),90.90% -115.31%(RSD=9.38% ,n=6),92.16% -105.70%(RSD=6.19% ,n=6), 90.81%-113.99%(RSD=8.86%,n=6),91.63%-102.79%(RSD=5.09%,n=6). The phenomenon that heavy metals of sample from Shandong and Hebei exceed the standard was the most serious. Main heavy metal which exceed the standard was Pb. The con-tent of Pb in Chamber was higher than other parts. CONCLUSIONS: The method is simple,precise,stable and reproducible,and can be used for simultaneous determination of 5 heavy metals in Vespae Nidus. There are different contents of heavy metal in Ves-pae Nidus of different producing areas and parts.

AIM:So far,endostatin is found playing the strongest effect to inhibit endothelial cells.Because endostatin protein is instable,it is difficult to prepare and apply.Gene therapy is used to assist endostatin to inhibit angiogenesis.This study constructed recombinant adenovirus of human endostatin gene using AdEasy-1 system to investigate the feasibility of expressing endostatin protein in ECV304 cells(human umbilical vein endothelial cells).METHODS:The experiment was performed at College of Life Science,Sun Yat-set University from March to October 2006.Endostatin was amplified from Pshuttle-Endostatin plasmid with PCR and subcloned into the pAdTrack-CMV shuttle vector after sequencing and identification.The resultant plasmid was co-transduced into E.coli BJ 5183 cells with pAdEasy-1 plasmid for homologous recombination.The recombinant plasmid was detected by restriction analysis and DNA sequence analysis.The linearized recombinant plasmid was transfected into AAV 293 cells with LipofectAMINE2000.The recombinant adenovirus was detected by GFP,PCR and restriction analysis.The virus was used to infect ECV304,and the expression of endostatin in vitro was detected by the techniques of RT-PCR and Western blot.RESULTS:The positive clones of the recombinants were constructed and confirmed by restriction endonuclease analysis and DNA sequence analysis.AAV293 cells were transfected.The titer of viral liquid was 2.06?1010 pfu/mL.The transcription of endostatin mRNA in ECV304 cells was checked by RT-PCR and endostatin protein could be detected in the culture by Western blot analysis.CONCLUSION:The recombinant adenoviral vector pAd-Endo carrying human endostatin is successfully constructed,and effective expression is found in ECV304 cells in vitro.