Objective: To construct the antheraea pernyi lysozyme(ApLZ) expression system using the methylotrophic yeast Pichia pastoris, to assay the antibacterial activity of the recombinant ApLZ against Streptococcus mutans. Methods:The ApLZ expression system was used in the expression and purity of Ap-lysozyme. The antibacterial activity of the recombinant ApLZ against Streptococcus mutans were assayed by using agar diffusion method. Results:Expression system of ApLZ was constructed using the methylotrophic yeast Pichia pastoris as a host. ApLZ was expressed correctly and secreted efficiently when the native signal sequence of ApLZ was used as secretion signal. The level of ApLZ expression in Pichia pastoris peaks at 96 h after the induction of sustaining 5 ml/L methanol. The molecular weight of the recombinant ApLZ is about 20 000. Conclusion:The recombinant ApLZ is active in inhibiting the growth of Streptococcus mutans.

Objective To investigate the clinicopathological features of primary hepatocellular carcinoma of the liver(PCCCL)and assess the over-all survival rate of PCCCL after hepatectomy and the association between prognosis and clear cell ratio in PCCCL.Methods The clinicopathological data of 43 patients with PCCCL treated with hepatectomy in our hospital from January 1999 to December 2003 were retrospectively analyzed.Results X2 test showed a positive rate of HCV infection(14.0%)and capsule formation(88.4 0A)in the PCCCL group markedly higher than the positive rate of HCV infection(1.2%)and capsule formation(65.0%)in the CHCC group(P=0.000 and 0.005).Meanwhile,the vascular invasion rate was notably Iower in the PCCCL group(53.4%vs.65.0%),but there were no significant differences between the two groups(P=0.129).Kaplan-Meier method showed that the1-,3-and 5-year survival rates were significantly higher in the PCCCL group than in the CHCC group(81%,53%and 39%vs.76%,46%and 32 0A,P=0.021).The prognosis of patients in the PCCCL group was related to capsule formation.vascular invasion,liver cirrhosis and clear cell ratio.The 1-,3-and 5-year survival rates were markedly higher in the group with higher clear cell ratio (≥75%,subgroup B)than in group with lower clear cell ratio(＜75%,subgroup A)(80 0A,43%and 23%vs.85 0A,77% and 68%,P=0.011).Conclusion The notable clinicopathologicaI features of the patients with PCCCL are higher rates of HCV infection.capsule formation and lower rate of vascular invasion.Their prognosis is better than the patients with CHCC and related with the ratio of clear cell.

Objective:To investigate the changes of hyoid position and pharyngeal airway before and after sagittal split ramus osteotomy (SSRO) of skeletal mandibular prognathism. Methods:Sixteen severe skeletal mandibular prognathism cases(Class Ⅲ, 5 males and 11 females) were chosen. Pre and post operation lateral cephalograms analysis reflecting pharyngeal airway and position of the hyoid bone was performed. Results: ① After SSRO, the width of airway decreased significantly. ② The distance between the mandible and the hyoid bone was also decreased significantly. No significant differences were found regarding measurements of the hyoid bone to the cranial base in male patients. Conclusion: The distance from the mandible to the hyoid bone and the width of airway were decreased significantly after SSRO. It suggests that the tongue and airway might be reformed functionally to new environments following SSRO surgical treatment.

Objective To explore the effect of quality control circle (QCC) on reducing the incidence of complications in the patients with peritoneal dialysis (PD). Methods Eighty-nine patients who underwent peritoneal dialysis after discharge were divided into QCC group (45 cases) and control group (44 cases) according to random sampling method. Both groups were followed up regularly, QCC activity was applied in patients of the QCC group on the basis of the QCC activity procedure. The intervention time was 1 year. The blood pressure, standard rate of urea clearance index, peritonitis incidence, catheter drift rate, infection incidences of external orifice of catheter, admission rate of complications and peritoneal dialysis withdrawal incidence between 2 groups were compared. Results After interference, the systolic and diastolic blood pressure in QCC group were significantly lower than those in control group:(124.11 ± 13.07) mmHg (1 mmHg=0.133 kPa) vs. (132.68 ± 12.09) mmHg and (72.13 ± 10.51) mmHg vs. (78.91 ± 11.79) mmHg, and there were statistical differences (P<0.05). The standard rate of urea clearance index in QCC group was significantly higher than that in control group:95.56%(43/45) vs. 81.82%(36/44), and there was statistical difference (P<0.05). After interference, the peritonitis incidence and admission rate of complications in QCC group was significantly lower than those in control group: 2.22% (1/45) vs. 13.64% (6/44) and 0 vs. 9.09% (4/44), and there were statistical differences (P<0.05). There were no statistical differences in catheter drift rate, infection incidences of external orifice of catheter and peritoneal dialysis withdrawal incidence between 2 group (P>0.05). The Spearman correlation analysis result showed that peritonitis was negatively correlated with education level (r = -0.28, P < 0.05), and standard rate of urea clearance index was positively correlated with education level (r = 0.21, P < 0.05). Conclusions The QCC management model can reduce the incidence of complications in patients with peritoneal dialysis.

In order to adapt to the development of society,an innovative talent cultivation system has been explored to train stomatological specialists with active innovation spirit and strong ability to practise.

Objective To introduce the general international method for estimation of lifetime risk of developed cancer,and to estimate the lifetime risk baseline values of several kinds of cancers related to radiation exposures in China.Methods The risk estimation was based on the data from Chinese Cancer Registry Annual Report (2010) and China Population & Employment Statistics Yearbook (2009),and made according to the method previously published by National Cancer Institute (NCI) in USA.Results The lifetime risk of all cancer in China in 2007 was estimated to be 27.77％,that of lung cancer 5.96％,that of breast cancer for female 3.34％,that of all leukemia 0.14％,that of thyroid cancer 0.37％.The lifetime risks of all cancer were estimated to be 32.74％ for males and 24.73％ for females,and that was 36.47％ for urban residents and 26.79％ for rural people.Conclusions The lifetime risk of all cancer for males in 2007 was about 1.25 times as much as that for females.The value of all cancer for urban residents was about 1.35 times as much as that for rural residents.The lifetime risk of developed cancers in 2007 in China is lower than that in the developed countries,such as Japan.

Objective To study the effects of diagnostic ultrasound combined with microbubble contrast agent on augmentation thrombolysis in vivo and to discuss the synergism and mechanism of combination of urokinase. Methods Thirty-two rabbits of femoral vein thrombus were divided into four groups: simple urokinase group, ultrasound and contrast microbubble group, ultrasound and contrast microbubble combined with urokinase group, control group. Then according to the best parameter combination which was definited in previous experiment, the experiments combination of urokinase were performed. Color Doppler was used to evaluate the recanalization of different group in 15 rain,30 min and 60 min. Results After the treatment of 15 rain and 30 min,the recanalization rate in ultrasound and contrast microbubble combined with urokinase group improved significantly compared with other groups (P<0.05);after 60 min, the recanalization grade of ultrasound and contrast microbubble and urokinase group was evidently better than other two group. The result of immunohistochemistry showed that disaggregation of clot and fibrous in this group at 60 min was better than the results at 15 min, 30 min. Conclusions Diagnostic ultrasound with microbubble contrast agent showed superior effects of thrombolysis and the synergism of combination of urokinase in vivo.

Objective We aimed to explore the expression of FHIT and investigate its correlation with clinicopathological features and prognosis of gallbladder carcinoma.Method Immunohistoehemieal detection of FHIT was performed on samples from 43 gallbladder carcinoma and 43 cholecystitis,and 2 years follow up was performed.Result FHIT protein was overexpressed in eholecystitis(x2=31.74,P=0.000).The overexpression was significantly related to differentiation and Nevin staging of gallbladder carcinoma(x2=4.47,P=0.035;x2=8.33,P=0.015);(rs=-0.56,P=0.031;rs=-0.68,P=0.014).Log-rank test showed that overexpression of FHIT had a good prognosis(x2=4.11,P=0.042).Cox analysis showed that expression of FHIT was all independent prognostic marker for postresectional survival of gallbladder carcinoma(RR:2.89,95%CI[2.46-3.32]).Conclusion Loss expression of FHIT closely correlated with clinicopathological features of gallbladder carcinoma.The expression of FHIT was an independent prognostic marker for post-resectional survival of gallbladder carcinoma.

Objective To profile the subtypes of open reading frame 75(ORF75)of human herpesvirus 8(HHV-8)in patients with Kaposi's sarcoma,and to evaluate their relationship with clinical phenotypes and invasiveness of Kaposi's sarcoma.Methods Twenty-five paraffin-embeded tissue specimens of Kaposi's sarcoma were collected in the Department of Dermatology.People's Hospiml of Xinjiang Uygur Autonomous Region.DNA was extracted from these specimens.and nested-PCR was performed to amplify HHV-8 DNA followed bv bi-directional sequencing.Phylogenetic analysis was carried out by using the software DNASTAR,Clustal W program and PHYLIP package so as to identify the ORF75 subtyoe of HHV-8.Results HHV-8 DNA was detected in 21(84%)out of the 25 samples,and 7 cases of AIDS-associated Kaposi's sarcoma were all positive for HHV-8.Among the 21 patients carrying HHV-8 DNA,18 were positive for subtype A ORF75.3 for subtype C ORF75.The ORF75 subtypes had no significant correlation with the presence of mucosal lesions or clinical phenotypes of Kaposi's sarcoma.Conclusions The majority of patients with Kaposi's sarcoma in Xinjiang are infected with HHV-8 of ORF 75 subtype A and C.The ORF75 subtypes of HHV-8 have no correlation with the presence of mucosal lesions or clinical phenotypes of Kaposi's sarcoma.

Asialogycoprotein receptor (ASGPR) is a specific receptor of mammalian hepatocytes,exclusively up-take the glycoprotein in the blood.The number of ASGPR correlates significantly with liver function,and the reduction of the number of ASGPR suggest liver dysfunction.Decreased ASGPR in patients with liver cirrhosis or portal hypertension or hepatocellar carcinoma,lead to varying degrees of liver dysfunction,which made it more susceptible to post operative complications.liver ASGPR scintigraphy with Technetium-99 m DTPA-galactosyl human serum albumin (99m Tc-GSA),combinating with single photon emission computed tomography (SPECT) technology can assess the functional reserve of remnant liver and predict incidence of postoperative complications,then assist to evaluate the use fulness for clinically surgical decisions.Current situation and progress of 99mTc-GSA SPECT imaging in hepatic surgery were reviewed in the paper.