Objective To introduce a rapid spot test method for nitrite in water to meet the urgent need of nitrite monitoring during accidental pollution. Methods According to the drinking water legislation in Europe (threshold: 0.10 mg/L NO2-), a rapid spot test method for nitrite in water was established by using an optimized composition of reagent mixture and a binary positive/negative response, conducted as follows: a tablet of a mixture of solid reagents containing sulfanilamide, N-(1-naphthyl) ethylene diamine dihydrochloride and sodium chloride was put on a spot test plate of Teflon mould, then, a drop of 30%(V/V) HCl and a drop of water sample were added on the reagent material. Results Under these conditions, all the test samples containing ≥0.10 mg/L NO2- can produce a positive response of the characteristic red-purple color, whereas, the samples will produce a negative response when the level of NO2- was below the limit. The results of real water samples determined with this new method were confirmed by using the photometric method based on the use of the same reagents. Conclusion The method is simple, rapid , accurate, and suitable for field monitoring of nitrite in water.

Animals ; Humans ; Reproducibility of Results ; Selenium ; analysis ; Spectrometry, Fluorescence ; Spectrophotometry, Atomic

Objective To explore the mechanism of Shenkangwan in preventing the development of diabetic nephropathy(DN)through TGF-?1/Smad signaling pathway.Methods The diabetic rat models were established by intraperitoneal injection of streptozotocin(STZ)in male Wistar rats and randomly divided into 3 groups:model control group,Capoten group and Shenkangwan group.Another 8 normal Wistar rats served as normal control.After the rats had been treated for 8 weeks,the mRNA expressions of fibronetin(FN)and TGF-?1 were detected by RT-PCR and the protein expressions of Smad 2,Smad 3 by immunohistochemistry in the renal tissues.Results During the past 8 weeks,the blood glucose of the rats in the model control group was all over 16.7 mmol/L and they exhibited the symptoms of diabetes mellitus,such as excessive thirsty,extreme hunger,frequent urination and unusual weight loss.The mRNA expressions of FN and TGF-?1 in rat renal tissues of model control group were significantly higher than those in the rats of normal control group(P

Objective:The purpose of the study was to investigate the effects of enteral nutrition on stress response and gut barrier function after surgery for esophageal cancer. Methods: Patients who underwent esophagectomy were divided into two groups. Group PN (28 patients) served as the control group. Group EN (26 patients) received enteral nutrition. Results: The serum total protein, albumin, prealbumin and transferrin decreased after operation, but there were no differences between the two groups. The serum levels of IL-6 were significantly higher on the first and third postoperative days after the operation in two groups (P

Objective: To investigate the effect of hypoxia/reoxygenation on endoplasmic reticulum stress in cultured neonatal rat cardiomyocytes. Methods: Neonatal rat cardiac myocytes in primary culture were exposed to hypoxia for 5.5 hours and subsequently reoxygenation for 2-24 hours. Western blot and RT- PCR were applied to monitor the expression change of GRP78(glucose regulated protein 78). 2-deoxy-D-glucose(2-DG) was the positive control of this study. Then Western blot and RT- PCR were used to examine the expression of GRP78. Results: Cell viability was decreased obviously after hypoxia/reoxygenation. Compared with untreated cells, the GRP78 content of the cells had increased significantly in the hypoxia/reoxygenation cells. The level of GRP78 protein and mRNA elevated from the points of 2 hours to 24 hours after reoxygenation, and increased most obviously at the point of 4 hours after reoxygenation.(4 hours: protein level 142% of the control, mRNA level 200%). 2-DG could induce the increasing expression of GRP78 in a concentration-dependent manner from 10-50 mmol/L. Conclusion: Hypoxia/reperfusion can induce endoplasmic reticulum stress in rat cardiomyocytes.

Objective: To compare the differences in gene expression between murine intestine intraepithelial lymphocytes (iIELs) and splenic T lymphocytes, establishing the cDNA subtractive library of iIELs, analyzing the iIELs special genes. Methods: The iIELs and splenic T lymphocytes were isolated by density gradient in Percoll and nylon column respectively. cDNA subtractive library of iIELs was established by improved subtractive hybridization. The cDNA was ligated with a different adaptor. After 2 rounds of hybridization and suppression PCR amplification, the PCR products were directly inserted into the plasmid vectors. The ESTs special for iIELs were screened by reverse Northern blot. Results: Fifty ESTs with obvious difference in the cDNA subtractive library of iIELs were screened. These ESTs were squenced and submitted to Genebank. The ESTs were analyzed through BLAST, and 14 genes special for iIELs were obtained. These genes were associated with the pattern recognition of pathogen, cytokine signal transduction and proteinase inhibitors. Conclusion: iIELs are important components of intestine natural immunity, it can recognize the conservative antigens of bacteria and can be affected by cytokines and proteinase inhibitors, leading to unique cellular behavior--more resting and less activation.

Objective To study the significance of dissection and exposure of the recurrent laryngeal nerve(RLN) in thyroid surgery.Methods The clinical data of 1 082 patients who had undergone operations on thyroid were retrospectively reviewed.All cases were routinely operated with dissection and exposure of RLN.Results Six cases had injury of RLN(0.5%),3 cases showed parathyroid insufficiency(0.3%)and 2 cases had injury of the superior laryngeal nerve(0.2%) ofter operation.The incidence of operative complications in thyroid surgery was closely related with if exposure of the RLN.Conclusions Exposure of the recurrent laryngeal nerve is a key measure to decrease injury of parathyroids,superior laryngeal nerve and recurrent laryngeal nerve during thyroid surgery.

The structure, pharmaceutical functions and extraction and isolation methods of chlorgenic acid were reviewed to provide references for the research of chlorogenic acids and the production of the medicines chlorogenic acids.

The prevalence of Toxoplasma gondii infection in free-range chickens in Jilin province, northeastern China was investigated. A total of 1095 serum samples were collected from nine administrative regions from July to October, 2012, and antibodies to T. gondii were examined by an in-house enzyme-linked immunosorbent assay (ELISA) using Toxoplasma lysate antigen (TLA). The detection results were confirmed by Western blot. The overall seroprevalence of T. gondii in free-range chickens was 17.6% (95% confidence interval [CI], 15.4–19.4%), ranging from 13.3% (95% CI, 6.3–20.4%) in Siping to 23.6% (95% CI, 15.7–31.6%) in Liaoyuan. There was no significant difference in T. gondii infection among different regions in Jilin province (P> 0.05). The widespread presence of T. gondii infection in freerange chickens of Jilin province implies the wide contamination with T. gondii oocysts in the living environment of people, and free-range chickens might be an important source of infection for humans.

Objective To analyse effect of nifedipine sustained tablet on hemorheology in the treatment of patients with hypertension complicated with coronary disease.Methods 70 cases with hypertension complicated with coronary disease February 2014 to February 2015, were randomly divided into observation group and control group.35 cases in each group.Observation group received nifedipine treatment, control group were treated with conventional therapy with aspirin, recorded and analyzed related indexes and clinical efficacy of two groups patients after treatment.Results After treatment, patients plasma viscosity and hematocrit values were (1.59 ±0.12)mPa? s, (0.42 ±0.02)%, higher than control group (1.69 ±0.13) mPa ? s, (0.46 ±0.03)%(P<0.05); after treatment whole blood viscosity in observation group (high shear) and whole blood viscosity (low shear) values were (4.95 ±0.41),(9.87 ±1.01) mPa? s, lower than control group (5.89 ±0.43),(10.76 ±0.89) mPa? s(P<0.05); After observation group total effective rate was 91.43%, higher than 71.43%( P<0.05 ) .Conclusion Effect of nifedipine sustained-release tablets in patients with hypertension complicated with coronary disease is sure, precise, and could be compared to conventional treatment alone.It has no significant adverse reactions, can effectively improve hemorheology in patients with hypertension, hypertension complicated with coronary disease.