Objective To introduce a rapid spot test method for nitrite in water to meet the urgent need of nitrite monitoring during accidental pollution. Methods According to the drinking water legislation in Europe (threshold: 0.10 mg/L NO2-), a rapid spot test method for nitrite in water was established by using an optimized composition of reagent mixture and a binary positive/negative response, conducted as follows: a tablet of a mixture of solid reagents containing sulfanilamide, N-(1-naphthyl) ethylene diamine dihydrochloride and sodium chloride was put on a spot test plate of Teflon mould, then, a drop of 30%(V/V) HCl and a drop of water sample were added on the reagent material. Results Under these conditions, all the test samples containing ≥0.10 mg/L NO2- can produce a positive response of the characteristic red-purple color, whereas, the samples will produce a negative response when the level of NO2- was below the limit. The results of real water samples determined with this new method were confirmed by using the photometric method based on the use of the same reagents. Conclusion The method is simple, rapid , accurate, and suitable for field monitoring of nitrite in water.

Animals ; Humans ; Reproducibility of Results ; Selenium ; analysis ; Spectrometry, Fluorescence ; Spectrophotometry, Atomic

To introduce composition,the testing principle of the urine analyzer,To reduce the failure rate, extend the service life of the instrument and improve the accuracy of diagnosis. Make the maintenance system ,according to the system of equipment maintenance maintain the urine analyzer daily and monthly. The maintenance plays an important role in reducing the failure rate, extending equipment life and improving the accuracy of diagnosis.

Objective　Exploring how to check the proportional hazards assumption of the Cox model,and the solutions to non-proportional hazards between the covariates and the hazard function.Methods　With a example data set of Ⅲc stage ovarian serous cystadenocarcina,illustrating how to use graphical methods to check the proportional hazards assumption of the Cox model.Results　the predictor of post-surgery administering medicine times violated the proportional hazards assumption of the Cox model.Conclusion　when using the Cox model to analyze the predictors of survival time,checking whether the predictors violate the proportional hazards assumption of the Cox model or not should be paid attention to.

Objective To study the clinical effect of surgery combined with antifungal drug in treatment of fungal nose-sinusitis.Methods The clinical data of 108 cases with fungal nose-sinusitis were retrospectively analyzed.All patients were treated with nasal endoscopic sinus surgery.According to whether the patients received antifungal drugs after low-temperature plasma-assisted surgery,the patients were divided into the treatment group and the control group.The treatment group(56 cases) was given antifungal fluconazole after low-temperature plasma-assisted endoscopic sinus surgery.52 cases in the control group were washed by 0.9％ sodium chloride solution after conventional endoscopic sinus surgery.The patients were followed up for 6 months to 2 years,the therapeutic effect and recurrence were compared between the two groups.Results 56 patients of the treatment group,49 cases were cured,7 cases improved,no recurrence.52 cases in the control group,47 cases were cured,improved in 4 cases,ineffective in 1 case,3 cases of recurrence.The recurrence rate of the treatment group was significantly lower than the control group (x2 =4.82,P ＜ 0.05).Conclusion The low-temperature plasma-assisted surgery combined with antifungal drug.in the treatment of fungal nose-sinusitis has exact efficacy,and has significant effect on the prevention and reduce the relapse rate,which worthy of clinical promotion.

Objective To explore the current education situation and demand of network teaching for health service management course so as to provide recommendations for improvement.Methods Questionnaire survey was conducted among 232 undergraduates from majors of preventive medicine,stomatology and clinical medicine.Totally 204 valid questionnaires were organized and recorded into database and SPSS 12.0 was employed to make descriptive statistical analysis of the data.Statistical results were used to show the current education situation and demand of network teaching for health service management course.Results 52.94％ (108/204) of the students interviewed were dissatisfied with current teaching mode.87.25％ (178/204) of the students believed the course website would be beneficial for learning.For the construction of the course website,health insurance system,health policy analysis and health organization system were considered both important and difficult.59.31％ (121/204) of the students showed that the course website should be updated at least once a week.Conclusions It is necessary to explore the network teaching mode for health service management course.Teaching reform depending on different professions and curriculums should be carried out for network teaching.

Objective To observe the effect of silencing the expression of ADM using RNA interfering technique on the expression ofβ-catenin and terminal differentiation of osteosarcoma cells.Methods After the intervention of 0 h,48 h and 72 h by ADM siRNA,we observed the change of distribution ofβ-catenin in F5M2 using immunocytochemistry staining.The expression levels of total and phosphorylatedβ-catenin and GSK3βwere detected by Western blot after the intervention by ADM siRNA.The F5 M2 cells treated with ADM siRNA were subjected to HE staining,alkaline phosphatase (ALP)assay and immunocytochemistry staining to investigate the biological effects of ADM siRNA on the morphology and terminal differentiation of F5M2 cells.Results After the intervention of 0 h,48 h and 72 h by ADM siRNA,the distribution ofβ-catenin was transferred from the cytoplasm to the nucleus.ADM siRNA downregulated the expression level of P-β-catenin and upregulated P-GSK3βdetected by Western blot.HE staining revealed that the configuration of F5M2 had undergone restorational changes similar to those of normal cells after ADM siRNA treatment.ALP assay and immunocytochemistry staining showed that the expression of the earlier and later molecular biomarkers of terminal differentiation,including ALP and osteocalcin were strongly positive.Conclusion Silencing the expression of ADM can activateβ-catenin to transfer to the nucleus from the cytoplasm,and induce osteosarcoma cells to make terminal differentiation through activatingβ-catenin signaling pathway.

Objective To study the effect and mechanism of Plantago depressa Willd. extract on rats with chronic nonbacterial prostatitis. Methods Sixty rats were divided into six groups randomly: normal control group, model control group, cernilton group, high-, medium- and low- dose Plantago depressa Willd. extract group, ten rats in each group. Chronic nonbacterial prostatitis rat model was established in the animals except of normal control group. Rats in the cernilton group were administrated with cernilton solution( 100 mg . kg-1 ) . High, middle and low dose Plantago depressa Willd. extract groups were given 150, 300 and 600 mg . kg-1 of Plantago depressa Willd. extract, respectively. The rats in the normal control and model control group were treated with the same volume of purified water.The administration was performed once every day, and lasted for three weeks.Effects of Plantago depressa Willd.extract on prostate index (PI), prostate specific antigen (PSA), tumor necrosis factorα ( TNF-α) , interleukin-1β ( IL-1β) , cycloxygenase 2 ( COX-2) , prostaglandin E2( PEG2 ) , transforming growth factor-β1( TGF-β1 ) , and connective tissue growth factor ( CTGF ) levels and pathological changes of prostate tissue in rats were observed. Results PI and PSA levels of the normal control group were(0.8±0.3)mg.g-1 and(106.5±10.6)pg.mL-1, respectively;those of the model control group were(2.2±0.2)mg.g-1 and(319.4±23.4)pg.mL-1 respectively;Those of the cernilton group were(1.6±0.3)mg.g-1 and(179.5±13.7)pg.mL-1 respectively;Those of the low-, medium- and high- dose Plantago depressa Willd.extract groups were(1.8±0.4),(1.3±0.3),(0.8±0.3)mg.g-1 and(263.4±28.6),(178.5±21.5), (143.5±12.9)pg.mL-1, respectively.Compared with the model control group, PI and PSA were significantly decreased(P<0.01) , TNF-α, IL-1β, COX-2, PEG2 , TGF-β1 and CTGF levels in the prostate tissues were decreased ( P<0. 01 ) , and inflammatory cell infiltration and fibrosis of prostate tissue was significantly alleviated in the model control group. Conclusion This study confirms Plantago depressa Willd.extract exerts effective therapeutical effect on chronic nonbacterial prostatitis in rats.

Objective To explore the mechanism of Shenkangwan in preventing the development of diabetic nephropathy(DN)through TGF-?1/Smad signaling pathway.Methods The diabetic rat models were established by intraperitoneal injection of streptozotocin(STZ)in male Wistar rats and randomly divided into 3 groups:model control group,Capoten group and Shenkangwan group.Another 8 normal Wistar rats served as normal control.After the rats had been treated for 8 weeks,the mRNA expressions of fibronetin(FN)and TGF-?1 were detected by RT-PCR and the protein expressions of Smad 2,Smad 3 by immunohistochemistry in the renal tissues.Results During the past 8 weeks,the blood glucose of the rats in the model control group was all over 16.7 mmol/L and they exhibited the symptoms of diabetes mellitus,such as excessive thirsty,extreme hunger,frequent urination and unusual weight loss.The mRNA expressions of FN and TGF-?1 in rat renal tissues of model control group were significantly higher than those in the rats of normal control group(P

The prevalence of Toxoplasma gondii infection in free-range chickens in Jilin province, northeastern China was investigated. A total of 1095 serum samples were collected from nine administrative regions from July to October, 2012, and antibodies to T. gondii were examined by an in-house enzyme-linked immunosorbent assay (ELISA) using Toxoplasma lysate antigen (TLA). The detection results were confirmed by Western blot. The overall seroprevalence of T. gondii in free-range chickens was 17.6% (95% confidence interval [CI], 15.4–19.4%), ranging from 13.3% (95% CI, 6.3–20.4%) in Siping to 23.6% (95% CI, 15.7–31.6%) in Liaoyuan. There was no significant difference in T. gondii infection among different regions in Jilin province (P> 0.05). The widespread presence of T. gondii infection in freerange chickens of Jilin province implies the wide contamination with T. gondii oocysts in the living environment of people, and free-range chickens might be an important source of infection for humans.