Objective To compare the performance of 2009 and 2010 reference standards of the United States Clinical and Laboratory Standards Institute(CLSI)in antimicrobial susceptibility for Enterobacteriaceae.Methods The breakpoints of susceptibility for ceftriaxone,ceftazidime and aztreonam in CLSI M100-S19(2009)were used to analyze results of antimicrobial susceptibility of Escherichia coli (308 isolates)and Klebsiella pneumoniae(194 isolates),as well as those revised in M100-S20(2010).Results With both the breakpoints in CLSI M100-S19(2009)and CLSI M100-S20(2010),proportions of antimicmbial resistance of ceftriaxone,ceftazidime and aztreonam for isolates of extended-spectrum β-lactamases(ESBLs)-producing E. coli were 84.2 percent vs.98.0 percent and 84.9 percent vs.98.0percent,respectively:those for isolates of ESBLs negative E. coli were 4.2 percent vs.7.7 percent and 5.6percent vs.8.4 percent,respectively;those for isolates of ESBLs positive Klebsiella spp.were 67.2 percent vs.90.2 percent and 67.2 percent vs.90.2 percent,respectively:and those for isolates of ESBLs negative Klebsiella spp.were 9.8 percent vs.10.6 percent and 10.6 percent vs.10.6 percent,respectively.Conclusions Proportions of antimicrobial resistance for ceftriaxone,ceftazidime and aztreonam with the rcference breakpeints of CLSI M100-S20(2010)increase to various extent,8.8 compared to those with the old ones in 2009.Compared with ESBLs negative isolates,ESBLs positive isolates are affected much more by the breakpeints of CLSI M100-S20(2010).Proportion of affected Klebsiella spp.by the revised breakpoints of CLSI M100-S20(2010)for ceftazidime and aztreonam is higher than that of E. coli with ESBLs positive.

The effects of SLG on the immune function of normal and immune-suppressed mice were investigated. The results showed that SLG (50mg/kg, im) could correct the suppressive effect of cyclophosphamide, dexamethasone or prednisolone on spleen and. thymus weight, serum specific antibody level and lymphocyte transformation. SLG could also improve the phagocytic activity of peritoneal macrophages and enhance peripheral blood ANAE+-lymphocyte ratio of mice. No obvious effect of SLG on the normal mice were observed except that it could increase the antibody level of normal mice. The results implicate that SLG may have some immunomodulatory effect.

Objective Sanatoriums have many complex business systems,so there is an urgent need for safe,reliable and stable operation of data centers to provide uninterrupted data services for its business systems. Methods Based on Web service heterogeneous system integration technology,data crawling robot technology,data center core technologies related to the data center,data center of sanatoriums was built. Results Sanatorim data center is the foundation on the integration platform of information,it is an effective solution to application integration and data sharing to achieve data consistency,timeliness,completeness,safety,effectiveness and accuracy. Conclusion The relative concentration of data center in the building of sanatorium is necessary in the development of sanatorium.

Embryonal tumors of central nervous system (CNS) are highly invasive malignant tumors that mainly affect infants.Even if the current active treatment strategies are given, the prognosis is still very poor.The 2016 World Health Organization(WHO) classification integrates the genetic information of diseases with histopathology, so as to make the classification and diagnosis of these tumors more accurate.In the new classification, embryonal tumors of CNS include the most common medulloblastoma, and the rare embryonal tumor with multilayered rosettes, C19MC-altered, embryonal tumor with multilayered rosettes, not otherwise specified (NOS), medulloepithelioma, CNS neuroblastoma, CNS ganglioneuroblastoma, CNS embryonal tumor, NOS, atypical teratoid rhabdoid tumor, and CNS embryonal tumor with rhabdoid features.Further study and exploration on the biological nature of these rare brain tumors will provide scientific basis for new treatment methods.

Since the tumor necrosis factor-related apoptosis-inducing ligand was found as a suitable agent for anticancer therapies,study on tumor research has been attaching more importance to it.Utilized the progress on the study of TRAIL on pancreatic carcinoma aspect recent years and standed the principle in TRAIL inducing pancreatic carcinoma apoptosis,this article first simply reviewed the resistance theory of TRAIL on pancreatic carcinoma.Following the trail of identified resistance theory,carring the method of increasing TRAIL's sensibility with protein inhibition,signal regulation,ect,some new treatments have been received,which are described along with the antitumor effect of TRAIL combined with mature treatment in pancreatic carcinoma,and simply assessed at last in the article,to provide a reference on the synergistic cytotoxic effects of TRAIL combined with valid drugs on panreatic carcinoma.

Objective To study the expression of homocysteine in serum of patients with common malignant tumor,and initially investigate the possibility of serum homocysteine as cancer biomarker.Methods Expression levels of homocysteine and caner biomarkers (CEA,AFP,CA125,CA199) in serum of 180 patients with established malignant tumor and 30 healthy controls (control) were measured,the results of homocysteine were compared with that of the cancer biomarkers based on the cutoff value used in clinic.Results The expression levels of homocysteine was significantly higher in patients with malignant tumor than in controls [(13.89 ± 4.95) μmol/L-(21.40 ± 9.38) μ mol/L vs (11.40 ± 3.13) μmol/L,P ＜ 0.05)].Conclusions The positive predictive rate of homocysteine is higher than the four kind of cancer biomarkers in lung cancer,breast cancer,esophageal cancer.The increase of homocysteine in tumors may be universal,and Homocysteine may be used as cancer biomarker in lung cancer,breast cancer and esophageal cancer.

Objective To investigate the prevalence of anti-HCV positive among volunteer blood donors in Zhouk-ou city. Methods A total of 200 950 blood specimens from voluntary blood donors in Zhoukou from 2007 to 2012 were detected for anti-HCV by double-antibody sandwich enzyme-linked immunosorbent assay,results were ana-lyzed. Results The total positive rate of anti-HCV was 0.21% (416/200 950),there were significant difference in positive rates of anti-HCV among populations of different genders,ages and educational background(χ2= 24.01 , 142.31,45.8,respectively,allP<0.01),the positive rate of anti-HCV in female was higher than that of male ([0.27% ,205/75 658]vs [0.17% ,211/125 292]);the positive rate of anti-HCV was the lowest in donors aged 21-30 years (0.06% ,38/63 719),and highest in donors aged>40 years (0.33% ,162/48 871);the lower donors re-ceived education,the higher positive rate of anti-HCV they had,the positive rate of anti-HCV in donors with j unior high school education was 0.26% (107/41 154). The positive rate of anti-HCV in blood donors decreased gradually from 2007 to 2012(χ2= 14.576,P<0.05).Conclusion The positive rate of anti-HCV is low in voluntary blood do-nors in Zhoukou and is significantly different among different populations.

After the concept of data curation and its application in foreign countries were described , the key points of data curation for data service in special libraries and related problems were analyzed according to the overall needs and changes of users in special libraries.

At the concentrations of 10-8mol/L, 5.5?l0-8mol/L, 10-7mol/L morphine produced a dose-dependent increase of beating rate in cultured rat heart cells with 9%,32%and 81% respectively. The increase was antagonized by opiate antagonist naloxone ( 10-5mol/L ) , but not affected by propranolol ( 10-5mol/L ) which could cancel the positive chronotropy of isoprenaline ( 10-9mol/L ) on the beating cells. The results support the concept that morphine may be a direct effect on myocadium, perhaps through opiate receptors.

BACKGROUND: D-dimer is the specific marker of cross-link fibers degraded by fibrinolysin. During cerebral hemorrhage, D-dimer is significantly increased; its decrease may reflect the absorption process of hematoma and be closely related with the substantial injuries of brain. Endothelin is remarkably increased during cerebral hemorrhage, which may result in ischemic necrosis of neural histiocytes. The endothelin volume in cerebral hemorrhagic patients can reflect the severity of illness and the function prognosis.OBJECTIVE: To observe the effect of xingnaojing he shengmai injection (compound injection) on D-dimer and endothelin in patients at acute stage of cerebral hemorrhage.DESIGN: A randomized and controlled trial.SETTING: Emergent Internal Department of the Affiliated Hospital of Medical College of Qingdao University and Department of Pathology of Jinan Military General Hospital.PARTICIPANTS: A total of 44 patients of inspected patients at acute stage of cerebral hemorrhage were collected in Emergent Internal Department of affiliated Hospital of Medical College of Qingdao University from September 1999 to May 2001, which were randomized into two groups, named as xingnaojing he shengmai injection(compound injection) group(23 patients) and xingnaojing group(21 patients).METHODS: ELISA method was applied to determine the level of D-dimer and endothelin of peripheral blood before and after treatment. SPSS 8.0 statistical software was applied to analyze the data.MAIN OUTCOME MEASURES: Effects of xingnaojing he shengmai injection(compound injection) on level of D-dimer and endothelin in patients at acute stage of cerebral hemorrhage, compare with xingnaojing group.RESULTS: Xingnaojing he shengmai injection(compound injection) significantly reduced the level of both D-dimer[ (0. 67 ± 0. 21), (0.34 ± 0.18) mg/L,P ＜ 0.01 ] and endothelin[ (94.52 ±30. 23), (51.30 ± 21.62) ng/L, P＜ 0.01 ] before and after treatment respectively. The improvement of which was obviously superior to those in xingnaojing group[ (0. 33 ±0. 11),(0.20±0.08)mg/L, P ＜0.01; (43.22±12.06), (26.52±8.13)ng/L respectively, concerning to the improvements in two groups, P ＜ 0. 01 ].CONCLUSION: To improve the fibrinolytic state in patients with cerebral hemorrhage may protect the substantial injuries of brain and prevent the secondary damage after cerebral hemorrhage. To inhibit cerebral vascular spasm after hemorrhage may improve ischemic oxygen deficiency in brain tissue and probably be taken as one of the ways for xingnaojing he shengmai injection giving play to its therapeutic effects.