1.ERIC-PCR Fingerprinting Related with oprD_2 Gene of Pseudomonas aeruginosa Isolates Resistant to Imipenem
Yang CAO ; Dianjun WEI ; Mc DIALLO ; Quanling MA ; Yang LIU
Chinese Journal of Nosocomiology 2006;0(12):-
0.05).Thirty genotypes were got from 54 strains by ERIC-PCR.CONCLUSIONS There isn′t significant difference on the absence rate of oprD2 gene in Tianjin and it gives us a hint that other factors can also result in the prevalence of IRPA.The existence of prevalence of IRPA clone in some departments advises us to effectively control the IRPA in hospital.
2.Dynamics of IL-2 and IL-7 levels during Highly active antiretroviral therapy and their significance
Ma DIALLO ; Xia CHEN ; Yuhuang ZHENG ; Yah HE ; Bo HE ; Huaying ZHOU ; Zi CHEN ; Yan LUO ; Si ZENG
Journal of Chinese Physician 2012;14(3):308-312,317
Objective The objective of this study was to investigate the course of certain common gamma cytokines ( IL-2 and IL-7 ) and their role on the control of the viral infection in a short term antiviral therapy.Methods A total of 35 adults with chronic HIV infection,responding to combined antiretroviral therapy (cART) guideline criteria were enrolled in this one year follow-up study.After signing an informed consent,20 ml blood were collected from each patient at base line,week 0,week 24 and week 48.1 ml serum collected from each patient was kept at -80 * C until use.Serum concentration of IL-2 and IL-7 was determined using ELISA kit from ebioscience Beijing.CD4 and CD8 cells were counted and quantified using flux cytometry,and serum HIV RNA was quantified using real time PCR.Results All patients had a mean baseline IL-2 level [ (9.67 ± 2.6 ) pg/ml ]lower than the controls [ ( 27.36 ± 5.05 ) pg/ml ].After treatment for 48 weeks,IL-2 increased[ ( 19.8 ± 3.3 ) pg/ml ].However,the mean baseline 1L-7 [ ( 81.74± 20.47 ) pg/ml ]in patients was higher than controls [ ( 2.06 ± 1.52 ) pg/ml ].After treatment for 48 weeks,IL-7 decreased [ (8.36 ± 2.16)pg/ml ].IL-2 showed a significant increase and positive correlation with CD4 cells after HAART initiation (0week:R =0.21,P =0.063,24week:R =0.24,P =0.033,48week:R =0.19,P =0.103; IL-7 showed a significant decrease after HAART initiation but it did not show correlation with CD4 cells.We noted there was a negative correlation between IL-2 and CD4 count in HAART baseline (R =0.28,P =0.012 ),but no correlation between IL-7 and CD4 count from 6 month after HAART.IL-2 showed negative correlation with HIV RNA ( R =- 0.17,P =0.032),but IL-7 showed a relationship with the HIV RNA Conclusions The increase of IL-2 coupled with the decrease of IL-7 revealed a partial restoration of immune response during HAART.However,the absence of relationship with HIV RNA suggested that these cytokines might not be directly involved in the reduction of viral load.