Objective To study the expansion and purification of human retinal progenitors cultured in vitro.(Methods Human) embryonic retinal progenitor cells(RPCs) were cultured.The characteristics of neural stem cell were observed.The expression of Nestin was examined flow cytometry(FCM) for the comparsion between the primary and secondary passages.The differentiation of RPCs had been observed after that they had been cultured in different conditions.Results Human RPCs had proliferated and formed cell clusters with a shape of neurosphere.After digestion,the scattered progenitors formed secondary neurosphere-like cell clusters.The cells in those clusters expressed Nestin and were negative for the mature retinal cell proteins.After spontaneous differentiation,the retinal progenitors expressed special proteins of mature retinal cells,including neuron specific enolase(NSE),glial fibrillary acidic protein(GFAP),protein kinase C(PKC) and Syntaxin-1.The expression of Nestin in the secondary culture passage was(39.3?18.9)%,which was significantly higher than that of the primary passage [(28.7?17.7)%](P

Objective To study the protective effect of Qidan Tongmai Tablet (QDTMT) on the (injury) induced by acute myocardial ischemia. Methods Acute myocardial ischemia was induced by sc isoproterenol (Iso, 5 mg/kg) into SD rats pretreated with ig QDTMT (2 g/kg) for two weeks. Activities of Ca~(2+)-(ATPase) in myocardial mitochondria and content of Ca~(2+) in myocardium and myocardial mitochondria were determined by colorimetric method. The changes of myocardial ultrastructure were investigated by means of transmission electron microscope. Results Compared with the controls, activities of Ca~(2+)-(ATPase) in mitochondrion were strengthened in QDTMT group and the content of Ca~(2+) was decreased ((P

The paper discusses the importance of bioethics,which is a love of life.Bioethics is the ethics of love.Three parts are included in the paper: love is foundation of bioethics;love is core of existence of bioethics;love impels bioethics to further develop.Love instructs the choice of objective bioethics in the toleration circumstance.Furthermore,love can impel the construction of bioethics in the progress of medical beneficence development.

Objective To explore the association between epicardial adipose tissue (EAT) thickness and left ventricular remodeling,left ventricular dysfunction in dilated cardiomyopathy (DCM).Methods One hundred and sixteen patients with DCM (DCM group) and 76 healthy subjects (control group) were examined by ultrasoundcardiogram.Left ventricular end-systolic diameter (LVESD),left ventricular end-diastolic diameter (LVEDD),left ventricular end-systolic volume (LVESV),left ventricular end-diastolic volume (LVEDV),left ventricular end-systolic volume index (LVESVI),left ventficular end-diastolic volume index (LVEDVI),left ventricular ejection fraction (LVEF) and EAT thickness were measured or calculated,and the relationship was evaluated.Results EAT thickness in DCM group was significantly lower than that in control group [(4.7 ± 1.2) mm vs.(7.6 ± 2.1) mm],and there was statistical difference (P ＜ 0.05).The linear correlation analysis results showed there was positive correlation between EAT thickness and LVESD,LVEDD,LVESV,LVEDV,LVEDVI,LVESVI (r =0.236,0.220,0.245,0.256,0.282,0.279,P ＜ 0.05),and there was no relationship between EAT thickness and LVEF (r =0.134,P ＞0.05).Conclusions In patients with DCM,there is a correlation between EAT thickness and ventricular remodeling.There is no correlation between EAT thickness and left ventricular dysfunction.

Radiotherapy is an important treatment for malignant tumors, but it will have a negative impact on the reproductive function of young female patients.In recent years, the measures to protect female fertility are increasing, including clinical mature technologies such as embryo cryopreservation and mature oocyte cryopreservation; highly experimental technologies such as immature oocyte in vitro fertilization and ovarian tissue cryopreservation and transplantation.In addition, the application of stem cells and the proposal of artificial ovary also show great prospects in the protection of female fertility.This paper reviews the research progress of female fertility protection strategies in radiotherapy.

Objective To explore the effects of cannabinoid receptor agonist WIN55,212-2 preconditioning on spinal cord ischemia reperfusion injury in rats.Methods A total of 32 male Sprague-Dawley rats was randomly divided into four groups (n =8):sham group,control group,dimethyl sulfoxide(DMSO) group which was given intraperitoneally DMSO 0.3 ml 30 min before ischemia reperfusion,and WIN group which was given intraperitoneally WIN55,212-2 1 mg/kg 30 min before ischemia reperfusion.Each rat was neurologically assessed at 24 h and 48 h after reperfusion by Tarlov scale,and the number of normal motor neurons at anterior horn of the spinal cord was recorded.Res uits The Tarlov scale of WIN group was significantly higher than that control and DMSO groups (P ＜ 0.05).There were more normal motor neurons at anterior horn of the spinal cord in WIN group than those in control and DMSO groups (P ＜ 0.05).Conclusions Cannabinoid receptor agonist WIN55,212-2 preconditioning might attenuate spinal cord ischemia reperfusion injury.

The establishment of open-loop psychiatric ward is an innovation for mental disease treatment,while new legal and ethical issues become evident.For instance,psychiatric patients with no guardians or agents can not normally sign the informed consent form at hospitalization.It is extremely difficult to make a definite diagnosis in some cases.Informed consent form is refused by certain guardians due to the misunderstanding of psychiatric diseases.This paper explores those legal and ethical issues arising in the medical treatment in open-loop psychiatric ward,and comes up with relevant countermeasures.

Objective To analyze thoroughly the related dates in internal and external hospital through data warehouse system.The valuable information is obtained to provide accurate and user-friendly decision support for the hospital.Methods The overall framework of data warehouse system and its key aspects are discussed with using Analysis Services tools for the design of the specific data warehouse system.Results To the HIS data sources and the specific needs of hospital data warehouse,many kinds of themes are designed including the patients component analysis,the component analysis of patients cost,the single disease analysis,cost-benefit analysis and comparative analysis of costs over the same period.Conclusion The data technology has reference value that promotes the application of data warehouse theory in hospital.

Objective:To study the insulin resistance and leptin resistance in women with polycystic ovary syndrome(PCOS).Methods:Body mass index(BMI),serum leptin,fasting blood glucose(FBG),fasting insulin(FINS),serum triglyceride,total cholesterol,high-density lipoprotein,low-density lipoprotein and insulin resistance index(HOMA-IR) were assessed in 30 normal women,30 simple obesity women and 41 PCOS women.Results:①The PCOS patients showed higher levels of BMI,serum leptin,FINS,HOMA-IR and low-density lipoprotein,and lower levels of high-density lipoprotein than that in normal control.Compared to simple obesity women,there only showed higher FINS level and lower BMI in PCOS women.②Serum leptin levels in PCOS patients were concerned with BMI、FINS、HOMA-IR and serum triglyceride levels.③There were no significant difference of the FINS and HOMA-IR between normal women and non obese PCOS women,but the obese PCOS women showed higher levels of FINS and HOMA-IR than that in simple obesity women;the serum leptin levels showed no significant difference between normal women and non-obese PCOS women,simple obesity women and obese PCOS women. Conclusion:PCOS patients may have insulin resistance and leptin resistance;There may have regulative effect between leptin resistance and insulin resistance in PCOS patients;There are direct relationship between insulin resistance and PCOS;The rise of serum leptin level in PCOS women may be the assident manifestation of obesity.

Objectives To observe the effect of flurbiprofen as preemptive analgesic and it's relative adverse reactions in pa-tients undergoing selective craniotomy .Methods Sixty patients undergoing selective craniotomy were divided into two groups randomly by age,sex,location of craniotomy:observation group (Group-flurbiprofen) and control group (Group-saline).Monitored sonoclot signal and estimate bleeding amount during the operation .Evaluated the quality of emergence after operation .Recorded the VAS pain scores 2 hours,6 hours,12 hours and 24 hours after operation ,and the occurrence of adverse reactions as nausea , vomiting and respiratory de-pression.Results The concentration of prostaglandin E2(PGE2) in the serum of the observation group was lower than that of control group( P <0.05).There were no significant differences in ACT (activated coagulation time) and CR (clot rate) of the two groups( P>0.05),but the PF (platelet function) of observation group was lower than that of control group ( P <0.05).There were no signifi-cant differences in awakening time and extubation time of the two groups ( P >0.05).The 2 hours'pain scores of the two groups was of no significant difference( P >0.05),but the pain scores of observation group were lower than that of control group in 6 hours,12 hours and 24 hours after operation( P <0.05).The occurrence of nausea , vomoting and respiratory depression had no significant differences ( P >0.05) .Conclusions Flurbiprofen as preemptive analgesic can reduce the postoperative pain in patients undergoing cranioto -my,without increasing the occurrence of nausea , vomit and respiratory depression .It can effect the platelet function ,but could not in-crease the amount of bleeding .