Objective To investigate surveillance of antimicrobial resistance of Enterobacteriaceae bacteria in the first affiliated hospital of Xinjiang Medical University.Methods Enterobacteriaceae in clinical specimens of 2013-2015 years in our hospital were collected.The clinical isolates were identified by the automatic bacterial identification analyzer.The drug sensitivity was determined by Automatic microbiological analyzer.The K-B paper method was used tosubsidiary experiment.VITEK-2 compact and VITEK-MS and the susceptibility test was carried out.The KB paper method was supplemented.Results The 11156 strains of Enterobacteriaceae were detected in 3 years,accounting for 65.6％ of gram-negative bacilli,the first of E.coli accounted for 42％,the second Klebsiella pneumoniae accounted for 31％,the third Enterobacter cloacae accounted for 8％.Respiratory tract is the common infection of Enterobacteriaceae accounted for 36％,followed by 34％ of the urethra.The resistant rate of Escherichia coli on imipenem,meropenem,cefoperazone/sulbactam,piperacillin/tazobactam,cefotetan,amikacin and nitrofurantoin were less than 10％.The drug resistance rate against Klebsiella pneumoniae from high to bttom in turn was imipenem,cefotetan,piperacillin/tazobactam,meropenem,amikacin.The detection rate of ESBL-producing Escherichia coli was higher than that of Klebsiella pneumoniae from 2013 to 2015 (41.8％,28.4％;66.4％,41.0％;71.3％,52.4％).The detection rate of carbapenem-resistant Klebsiella pneumoniae was 2.3％ ％,higher than Escherichia coli of 0.6％.Conclusion Multidrug resistant bacteria and Enterobacteriaceae detection rate increased year by year.