The present study was designed to examine the positive impact of collagen and glucosamine hydrate supplementation on joint markers, matrix metalloproteinase-3 (MMP-3), tissue inhibitor of metalloproteinases-1 (TIMP-1) and keratane sulfate (KS) . Thirteen healthy fun-runners supplemented collagen and glucosamine hydrate for 30 days. Blood samples were collected on days 0, 15 and 30. The test-drink supplement, however, significantly decreased MMP-3 and KS on days 15 and 30. These results suggest that the supplementation of collagen and glucosamine hydrate may prevent injury and inflammation of joint cartilages and be useful for joint conditions.